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Functional alterations in cerebrovascular K(+) and Ca(2+) channels are comparable between simulated microgravity rat and SHR.
Am J Physiol Heart Circ Physiol. 2005 Sep; 289(3):H1265-76.AJ

Abstract

Exposure to microgravity leads to a sustained elevation in transmural pressure across the cerebral vasculature due to removal of hydrostatic pressure gradients. We hypothesized that ion channel remodeling in cerebral vascular smooth muscle cells (VSMCs) similar to that associated with hypertension may occur and play a role in upward autoregulation of cerebral vessels during microgravity. Sprague-Dawley rats were subjected to 4-wk tail suspension (Sus) to simulate the cardiovascular effect of microgravity. Large-conductance Ca(2+)-activated K(+) (BK(Ca)), voltage-gated K(+) (K(V)), and L-type voltage-dependent Ca(2+) (Ca(L)) currents of Sus and control (Con) rat cerebral VSMCs were investigated with a whole cell voltage-clamp technique. Under the same experimental conditions, K(V), BK(Ca), and Ca(L) currents of cerebral VSMCs from adult spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY) were also investigated. K(V) current density decreased in Sus rats vs. Con rats [1.07 +/- 0.14 (n = 22) vs. 1.31 +/- 0.28 (n = 16) pA/pF at +20 mV (P < 0.05)] and BK(Ca) and Ca(L) current densities increased [BK(Ca): 1.70 +/- 0.37 (n = 23) vs. 0.88 +/- 0.22 (n = 19) pA/pF at +20 mV (P < 0.05); Ca(L): -2.17 +/- 0.21 (n = 35) vs. -1.31 +/- 0.10 (n = 26) pA/pF at +10 mV (P < 0.05)]. Similar changes were also observed in SHR vs. WKY cerebral VSMCs: K(V) current density decreased [1.03 +/- 0.33 (n = 9) vs. 1.62 +/- 0.64 (n = 9) pA/pF at +20 mV (P < 0.05)] and BK(Ca) and Ca(L) current densities increased [BK(Ca): 2.54 +/- 0.47 (n = 11) vs. 1.12 +/- 0.33 (n = 12) pA/pF at +20 mV (P < 0.05); Ca(L): -3.99 +/- 0.53 (n = 12) vs. -2.28 +/- 0.20 (n = 10) pA/pF at +20 mV (P < 0.05)]. These findings support our hypothesis, and their impact on space cardiovascular research is discussed.

Authors+Show Affiliations

Xie MJ
Department of Aerospace Physiology, Fourth Military Medical University, Xi'an 710032, China.
Zhang LF
No affiliation info available
Ma J
No affiliation info available
Cheng HW
No affiliation info available

MeSH

AnimalsCalciumCalcium Channels, L-TypeCerebral ArteriesDisease Models, AnimalHypertensionLarge-Conductance Calcium-Activated Potassium ChannelsMaleMembrane PotentialsMuscle, Smooth, VascularPatch-Clamp TechniquesPotassium Channels, Calcium-ActivatedPotassium Channels, Voltage-GatedRatsRats, Inbred SHRRats, Inbred WKYRats, Sprague-DawleyTailWeightlessness Simulation

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15894580

Citation

Xie, Man-Jiang, et al. "Functional Alterations in Cerebrovascular K(+) and Ca(2+) Channels Are Comparable Between Simulated Microgravity Rat and SHR." American Journal of Physiology. Heart and Circulatory Physiology, vol. 289, no. 3, 2005, pp. H1265-76.
Xie MJ, Zhang LF, Ma J, et al. Functional alterations in cerebrovascular K(+) and Ca(2+) channels are comparable between simulated microgravity rat and SHR. Am J Physiol Heart Circ Physiol. 2005;289(3):H1265-76.
Xie, M. J., Zhang, L. F., Ma, J., & Cheng, H. W. (2005). Functional alterations in cerebrovascular K(+) and Ca(2+) channels are comparable between simulated microgravity rat and SHR. American Journal of Physiology. Heart and Circulatory Physiology, 289(3), H1265-76.
Xie MJ, et al. Functional Alterations in Cerebrovascular K(+) and Ca(2+) Channels Are Comparable Between Simulated Microgravity Rat and SHR. Am J Physiol Heart Circ Physiol. 2005;289(3):H1265-76. PubMed PMID: 15894580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional alterations in cerebrovascular K(+) and Ca(2+) channels are comparable between simulated microgravity rat and SHR. AU - Xie,Man-Jiang, AU - Zhang,Li-Fan, AU - Ma,Jin, AU - Cheng,Hong-Wei, Y1 - 2005/05/13/ PY - 2005/5/17/pubmed PY - 2005/9/29/medline PY - 2005/5/17/entrez SP - H1265 EP - 76 JF - American journal of physiology. Heart and circulatory physiology JO - Am J Physiol Heart Circ Physiol VL - 289 IS - 3 N2 - Exposure to microgravity leads to a sustained elevation in transmural pressure across the cerebral vasculature due to removal of hydrostatic pressure gradients. We hypothesized that ion channel remodeling in cerebral vascular smooth muscle cells (VSMCs) similar to that associated with hypertension may occur and play a role in upward autoregulation of cerebral vessels during microgravity. Sprague-Dawley rats were subjected to 4-wk tail suspension (Sus) to simulate the cardiovascular effect of microgravity. Large-conductance Ca(2+)-activated K(+) (BK(Ca)), voltage-gated K(+) (K(V)), and L-type voltage-dependent Ca(2+) (Ca(L)) currents of Sus and control (Con) rat cerebral VSMCs were investigated with a whole cell voltage-clamp technique. Under the same experimental conditions, K(V), BK(Ca), and Ca(L) currents of cerebral VSMCs from adult spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY) were also investigated. K(V) current density decreased in Sus rats vs. Con rats [1.07 +/- 0.14 (n = 22) vs. 1.31 +/- 0.28 (n = 16) pA/pF at +20 mV (P < 0.05)] and BK(Ca) and Ca(L) current densities increased [BK(Ca): 1.70 +/- 0.37 (n = 23) vs. 0.88 +/- 0.22 (n = 19) pA/pF at +20 mV (P < 0.05); Ca(L): -2.17 +/- 0.21 (n = 35) vs. -1.31 +/- 0.10 (n = 26) pA/pF at +10 mV (P < 0.05)]. Similar changes were also observed in SHR vs. WKY cerebral VSMCs: K(V) current density decreased [1.03 +/- 0.33 (n = 9) vs. 1.62 +/- 0.64 (n = 9) pA/pF at +20 mV (P < 0.05)] and BK(Ca) and Ca(L) current densities increased [BK(Ca): 2.54 +/- 0.47 (n = 11) vs. 1.12 +/- 0.33 (n = 12) pA/pF at +20 mV (P < 0.05); Ca(L): -3.99 +/- 0.53 (n = 12) vs. -2.28 +/- 0.20 (n = 10) pA/pF at +20 mV (P < 0.05)]. These findings support our hypothesis, and their impact on space cardiovascular research is discussed. SN - 0363-6135 UR - https://www.unboundmedicine.com/medline/citation/15894580/Functional_alterations_in_cerebrovascular_K_+__and_Ca_2+__channels_are_comparable_between_simulated_microgravity_rat_and_SHR_ DB - PRIME DP - Unbound Medicine ER -