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An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser-induced fluorescence detection.
Electrophoresis. 2005 Jun; 26(12):2384-90.E

Abstract

This paper reports the development of a method based on capillary electrophoresis with laser-induced fluorescence detection for the simultaneous determination of thiouracil (TU) and phenylthiouracil (PhTU) with high sensitivity (nanomolar range, i.e., attomoles detected). After derivatization with 5-iodoacetamidofluorescein, the analytes were separated by capillary zone electrophoresis using 20 mM phosphate buffer (pH 10.0) and quantified by fluorescence detection. The linearity range, precision, recovery, and detection limits were determined, and the method was shown to be applicable for the determination of TU and PhTU in spiked feed samples and urine.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Campus de Espinardo, Murcia, Spain. tpr@um.esNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

15895465

Citation

Pérez-Ruiz, Tomás, et al. "An Ultrasensitive Method for the Determination of Thiouracil and Phenylthiouracil Using Capillary Zone Electrophoresis and Laser-induced Fluorescence Detection." Electrophoresis, vol. 26, no. 12, 2005, pp. 2384-90.
Pérez-Ruiz T, Martínez-Lozano C, Sanz A, et al. An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser-induced fluorescence detection. Electrophoresis. 2005;26(12):2384-90.
Pérez-Ruiz, T., Martínez-Lozano, C., Sanz, A., & Galera, R. (2005). An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser-induced fluorescence detection. Electrophoresis, 26(12), 2384-90.
Pérez-Ruiz T, et al. An Ultrasensitive Method for the Determination of Thiouracil and Phenylthiouracil Using Capillary Zone Electrophoresis and Laser-induced Fluorescence Detection. Electrophoresis. 2005;26(12):2384-90. PubMed PMID: 15895465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser-induced fluorescence detection. AU - Pérez-Ruiz,Tomás, AU - Martínez-Lozano,Carmen, AU - Sanz,Antonio, AU - Galera,Raquel, PY - 2005/5/17/pubmed PY - 2005/9/20/medline PY - 2005/5/17/entrez SP - 2384 EP - 90 JF - Electrophoresis JO - Electrophoresis VL - 26 IS - 12 N2 - This paper reports the development of a method based on capillary electrophoresis with laser-induced fluorescence detection for the simultaneous determination of thiouracil (TU) and phenylthiouracil (PhTU) with high sensitivity (nanomolar range, i.e., attomoles detected). After derivatization with 5-iodoacetamidofluorescein, the analytes were separated by capillary zone electrophoresis using 20 mM phosphate buffer (pH 10.0) and quantified by fluorescence detection. The linearity range, precision, recovery, and detection limits were determined, and the method was shown to be applicable for the determination of TU and PhTU in spiked feed samples and urine. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15895465/An_ultrasensitive_method_for_the_determination_of_thiouracil_and_phenylthiouracil_using_capillary_zone_electrophoresis_and_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.200410252 DB - PRIME DP - Unbound Medicine ER -