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Environmental genotoxicity assessment of an urban stream using freshwater planarians.
Mutat Res. 2005 Aug 01; 585(1-2):79-85.MR

Abstract

Pollution is a major concern in urban areas. Due to its biological significance, genotoxicity should be a main focus for pollution biomonitoring, due mainly to the increasing complexity of the chemical environment in which organisms are exposed. Diluvio's Basin (Porto Alegre, RS, Brazil) is a heavily polluted urban ecosystem impacted by urban wastewater. Planarians are useful organism for evaluating environmental genotoxicity because of their high sensitivity, low cost, high proliferative rate and also because of their basal evolutionary position in relation to complex metazoans. Comet assay is a powerful and highly sensitive method of evaluating primary DNA lesions. Based on the unique features of planarians and the current environmental state of Diluvio's Basin, the aim of this work was to evaluate the genotoxic potential of this body of water using comet assay in planarians. Planarians were exposed to the water for 13 days in a laboratory and comet assay was performed in order to screen possible DNA damages. The results indicated an increasing gradient of damage towards basin's mouth. Such a gradient could be related to the gradual increase of pollutants among the different sample sites. Moreover, there seems to be a correlation between the urbanization gradient that exists within the watershed and the genotoxicity. Historical physical-chemical data was also gathered and examined for possible correlations with genotoxicity. Comet assay in planarians is a very promising test for environmental monitoring studies. Its application should be expanded.

Authors+Show Affiliations

Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. daniel_pra@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15897001

Citation

Prá, Daniel, et al. "Environmental Genotoxicity Assessment of an Urban Stream Using Freshwater Planarians." Mutation Research, vol. 585, no. 1-2, 2005, pp. 79-85.
Prá D, Lau AH, Knakievicz T, et al. Environmental genotoxicity assessment of an urban stream using freshwater planarians. Mutat Res. 2005;585(1-2):79-85.
Prá, D., Lau, A. H., Knakievicz, T., Carneiro, F. R., & Erdtmann, B. (2005). Environmental genotoxicity assessment of an urban stream using freshwater planarians. Mutation Research, 585(1-2), 79-85.
Prá D, et al. Environmental Genotoxicity Assessment of an Urban Stream Using Freshwater Planarians. Mutat Res. 2005 Aug 1;585(1-2):79-85. PubMed PMID: 15897001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Environmental genotoxicity assessment of an urban stream using freshwater planarians. AU - Prá,Daniel, AU - Lau,Adriana Helena, AU - Knakievicz,Tanise, AU - Carneiro,Flávia Rosa, AU - Erdtmann,Bernardo, PY - 2004/01/24/received PY - 2005/03/30/revised PY - 2005/04/14/accepted PY - 2005/5/18/pubmed PY - 2005/9/2/medline PY - 2005/5/18/entrez SP - 79 EP - 85 JF - Mutation research JO - Mutat Res VL - 585 IS - 1-2 N2 - Pollution is a major concern in urban areas. Due to its biological significance, genotoxicity should be a main focus for pollution biomonitoring, due mainly to the increasing complexity of the chemical environment in which organisms are exposed. Diluvio's Basin (Porto Alegre, RS, Brazil) is a heavily polluted urban ecosystem impacted by urban wastewater. Planarians are useful organism for evaluating environmental genotoxicity because of their high sensitivity, low cost, high proliferative rate and also because of their basal evolutionary position in relation to complex metazoans. Comet assay is a powerful and highly sensitive method of evaluating primary DNA lesions. Based on the unique features of planarians and the current environmental state of Diluvio's Basin, the aim of this work was to evaluate the genotoxic potential of this body of water using comet assay in planarians. Planarians were exposed to the water for 13 days in a laboratory and comet assay was performed in order to screen possible DNA damages. The results indicated an increasing gradient of damage towards basin's mouth. Such a gradient could be related to the gradual increase of pollutants among the different sample sites. Moreover, there seems to be a correlation between the urbanization gradient that exists within the watershed and the genotoxicity. Historical physical-chemical data was also gathered and examined for possible correlations with genotoxicity. Comet assay in planarians is a very promising test for environmental monitoring studies. Its application should be expanded. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/15897001/Environmental_genotoxicity_assessment_of_an_urban_stream_using_freshwater_planarians_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(05)00106-3 DB - PRIME DP - Unbound Medicine ER -