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Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline Comet assay.
Mutagenesis. 2005 Jul; 20(4):285-90.M

Abstract

The single cell gel electrophoresis or Comet assay is one of the most popular techniques for genotoxicity assessment. The present study was undertaken to validate our previously modified version of the Comet assay for genotoxicity assessment in Drosophila melanogaster (Oregon R(+)) with four well-known mutagenic and carcinogenic alkylating agents, i.e. ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), N-ethyl-N-nitrosourea (ENU) and cyclophosphamide (CP). Third instar larvae (74 +/- 2 h) of D.melanogaster were fed different concentrations of EMS, MMS, ENU and CP (0.05, 0.5 and 1.0 mM) mixed standard Drosophila food for 24 h. At 98 +/- 2 h, the anterior midgut from control and treated larvae were dissected out, single-cell suspensions were prepared and Comet assay was performed. Our results show a dose-dependent increase in DNA damage with all the four alkylating agents, in comparison to control. The lower concentration (0.05 mM) of the test chemicals, except MMS, did not induce any DNA damage in the gut cells of the exposed larvae. When comparison of Comet parameters was made among the chemicals, MMS was found to be the most potent genotoxicant and ENU the least. The present study validated our previous observation and shows that D.melanogaster is a sensitive and suitable model for the in vivo assessment of genotoxicity using our modified alkaline Comet assay.

Authors+Show Affiliations

Embryotoxicology Section, Industrial Toxicology Research Center, PO Box 80, M.G. Marg, Lucknow, 226001, Uttar Pradesh, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

15899934

Citation

Siddique, Hifzur R., et al. "Validation of Drosophila Melanogaster as an in Vivo Model for Genotoxicity Assessment Using Modified Alkaline Comet Assay." Mutagenesis, vol. 20, no. 4, 2005, pp. 285-90.
Siddique HR, Chowdhuri DK, Saxena DK, et al. Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline Comet assay. Mutagenesis. 2005;20(4):285-90.
Siddique, H. R., Chowdhuri, D. K., Saxena, D. K., & Dhawan, A. (2005). Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline Comet assay. Mutagenesis, 20(4), 285-90.
Siddique HR, et al. Validation of Drosophila Melanogaster as an in Vivo Model for Genotoxicity Assessment Using Modified Alkaline Comet Assay. Mutagenesis. 2005;20(4):285-90. PubMed PMID: 15899934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline Comet assay. AU - Siddique,Hifzur R, AU - Chowdhuri,D Kar, AU - Saxena,D K, AU - Dhawan,Alok, Y1 - 2005/05/17/ PY - 2005/5/19/pubmed PY - 2005/9/3/medline PY - 2005/5/19/entrez SP - 285 EP - 90 JF - Mutagenesis JO - Mutagenesis VL - 20 IS - 4 N2 - The single cell gel electrophoresis or Comet assay is one of the most popular techniques for genotoxicity assessment. The present study was undertaken to validate our previously modified version of the Comet assay for genotoxicity assessment in Drosophila melanogaster (Oregon R(+)) with four well-known mutagenic and carcinogenic alkylating agents, i.e. ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), N-ethyl-N-nitrosourea (ENU) and cyclophosphamide (CP). Third instar larvae (74 +/- 2 h) of D.melanogaster were fed different concentrations of EMS, MMS, ENU and CP (0.05, 0.5 and 1.0 mM) mixed standard Drosophila food for 24 h. At 98 +/- 2 h, the anterior midgut from control and treated larvae were dissected out, single-cell suspensions were prepared and Comet assay was performed. Our results show a dose-dependent increase in DNA damage with all the four alkylating agents, in comparison to control. The lower concentration (0.05 mM) of the test chemicals, except MMS, did not induce any DNA damage in the gut cells of the exposed larvae. When comparison of Comet parameters was made among the chemicals, MMS was found to be the most potent genotoxicant and ENU the least. The present study validated our previous observation and shows that D.melanogaster is a sensitive and suitable model for the in vivo assessment of genotoxicity using our modified alkaline Comet assay. SN - 0267-8357 UR - https://www.unboundmedicine.com/medline/citation/15899934/Validation_of_Drosophila_melanogaster_as_an_in_vivo_model_for_genotoxicity_assessment_using_modified_alkaline_Comet_assay_ L2 - https://academic.oup.com/mutage/article-lookup/doi/10.1093/mutage/gei032 DB - PRIME DP - Unbound Medicine ER -