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Development and evaluation of a latex agglutination test for the rapid diagnosis of scrub typhus.

Abstract

The purpose of this research was to develop a simple and rapid diagnostic test for scrub typhus using a latex agglutination test (LAT) to detect antibodies against Orientia tsutsugamushi. Five strains of O. tsutsugamushi were propagated in L929 cells. The rickettsiae were purified and concentrated with percoll density gradient centrifugation. A suitable concentration of O. tsutsugamushi soluble antigen was used to sensitize latex to prepare the latex antigen. The specificity, sensitivity, and accuracy of the latex antigen were assessed. The LAT, indirect immunofluorescent antibody test (IFA), and Weil-Felix agglutination test (WF) were compared by testing 109 acute febrile illness cases and 100 confirmed non-scrub typhus cases (50 other febrile disease cases and 50 healthy controls). By using the IFA as the standard reference method, the overall sensitivity, specificity, and accuracy of the LAT were 89.1, 98.2, and 93.6%, respectively. By contrast, the sensitivity of the WF, compared with the IFA, was only 47.3%, while the specificity and accuracy were 92.6 and 69.7%, respectively. Thus, the LAT described here is another important alternative test for the diagnosis of scrub typhus.

Authors+Show Affiliations

Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok. tmvwc@mahidol.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15906651

Citation

Wongchotigul, Varee, et al. "Development and Evaluation of a Latex Agglutination Test for the Rapid Diagnosis of Scrub Typhus." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 36, no. 1, 2005, pp. 108-12.
Wongchotigul V, Waicharoen S, Riengrod S, et al. Development and evaluation of a latex agglutination test for the rapid diagnosis of scrub typhus. Southeast Asian J Trop Med Public Health. 2005;36(1):108-12.
Wongchotigul, V., Waicharoen, S., Riengrod, S., Chimsumang, S., Rattanadakul, J., & Chinprasatsak, S. (2005). Development and evaluation of a latex agglutination test for the rapid diagnosis of scrub typhus. The Southeast Asian Journal of Tropical Medicine and Public Health, 36(1), pp. 108-12.
Wongchotigul V, et al. Development and Evaluation of a Latex Agglutination Test for the Rapid Diagnosis of Scrub Typhus. Southeast Asian J Trop Med Public Health. 2005;36(1):108-12. PubMed PMID: 15906651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and evaluation of a latex agglutination test for the rapid diagnosis of scrub typhus. AU - Wongchotigul,Varee, AU - Waicharoen,Sunthareeya, AU - Riengrod,Searmsap, AU - Chimsumang,Saichon, AU - Rattanadakul,Jaruwadee, AU - Chinprasatsak,Soontorn, PY - 2005/5/24/pubmed PY - 2005/8/23/medline PY - 2005/5/24/entrez SP - 108 EP - 12 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J. Trop. Med. Public Health VL - 36 IS - 1 N2 - The purpose of this research was to develop a simple and rapid diagnostic test for scrub typhus using a latex agglutination test (LAT) to detect antibodies against Orientia tsutsugamushi. Five strains of O. tsutsugamushi were propagated in L929 cells. The rickettsiae were purified and concentrated with percoll density gradient centrifugation. A suitable concentration of O. tsutsugamushi soluble antigen was used to sensitize latex to prepare the latex antigen. The specificity, sensitivity, and accuracy of the latex antigen were assessed. The LAT, indirect immunofluorescent antibody test (IFA), and Weil-Felix agglutination test (WF) were compared by testing 109 acute febrile illness cases and 100 confirmed non-scrub typhus cases (50 other febrile disease cases and 50 healthy controls). By using the IFA as the standard reference method, the overall sensitivity, specificity, and accuracy of the LAT were 89.1, 98.2, and 93.6%, respectively. By contrast, the sensitivity of the WF, compared with the IFA, was only 47.3%, while the specificity and accuracy were 92.6 and 69.7%, respectively. Thus, the LAT described here is another important alternative test for the diagnosis of scrub typhus. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/15906651/Development_and_evaluation_of_a_latex_agglutination_test_for_the_rapid_diagnosis_of_scrub_typhus_ L2 - http://www.diseaseinfosearch.org/result/7277 DB - PRIME DP - Unbound Medicine ER -