Tags

Type your tag names separated by a space and hit enter

Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction.
FEMS Immunol Med Microbiol. 2005 Jun 01; 44(3):311-6.FI

Abstract

Several discoveries about leprosy indicate that Mycobacterium leprae transmission mainly occurs by inhalation, and the nose is major port of entry and exit. The present study evaluated the clinical application of PCR for detection of M. leprae DNA in nasal mucosa biopsies in untreated leprosy patients (52) and their contacts (99) from the State Reference Center in Sanitary Dermatology and Leprosy, Uberlandia, MG, Brazil. PCR detection of a 372-base pair DNA fragment from M. leprae was accomplished in 36 (69.2%) patients, from which 34 (91.9%) of them were multibacillaries. Furthermore, PCR was positive in 3 (16.7%) of 18 slit-skin smear negative, 4 (25.0%) of 16 skin lesion BI negative, 8 (33.3%) of 24 nasal mucosa BI negative patients, and 10 of 99 contacts (10.1%). The presence of bacilli in 10.1% of the contacts may potentially reflect an occult leprosy, and these patients must be accompanied, followed by a chemoprophylaxy treatment. Considering all PCR results against clinical and BI classification of patients and controls, we have found a sensitivity of 69.2%, a specificity of 89.9%, and an accuracy of 82.8%. It has been demonstrated here through PCR of nasal biopsies that the bacillus invades the mucosa, passing through the nasal inferior turbinate to reach peripheral blood. Therefore, the molecular investigation of invasive nasal biopsies by PCR tests has proven to be useful in defining patients of higher risk of transmission and risk-group contacts, which is an important step to reach the World Health Organization objective towards the elimination of leprosy as a public health problem.

Authors+Show Affiliations

State Reference Center for Sanitary Dermatology and Leprosy, Clinical Hospital, Faculty of Medicine, Federal University of Uberlândia, Minas Gerais, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15907454

Citation

Patrocínio, Lucas Gomes, et al. "Detection of Mycobacterium Leprae in Nasal Mucosa Biopsies By the Polymerase Chain Reaction." FEMS Immunology and Medical Microbiology, vol. 44, no. 3, 2005, pp. 311-6.
Patrocínio LG, Goulart IM, Goulart LR, et al. Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction. FEMS Immunol Med Microbiol. 2005;44(3):311-6.
Patrocínio, L. G., Goulart, I. M., Goulart, L. R., Patrocínio, J. A., Ferreira, F. R., & Fleury, R. N. (2005). Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction. FEMS Immunology and Medical Microbiology, 44(3), 311-6.
Patrocínio LG, et al. Detection of Mycobacterium Leprae in Nasal Mucosa Biopsies By the Polymerase Chain Reaction. FEMS Immunol Med Microbiol. 2005 Jun 1;44(3):311-6. PubMed PMID: 15907454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction. AU - Patrocínio,Lucas Gomes, AU - Goulart,Isabela Maria Bernardes, AU - Goulart,Luiz Ricardo, AU - Patrocínio,José Antônio, AU - Ferreira,Frederico Rogério, AU - Fleury,Raul Negrão, PY - 2004/03/10/received PY - 2004/12/21/revised PY - 2005/01/05/accepted PY - 2005/5/24/pubmed PY - 2006/2/24/medline PY - 2005/5/24/entrez SP - 311 EP - 6 JF - FEMS immunology and medical microbiology JO - FEMS Immunol Med Microbiol VL - 44 IS - 3 N2 - Several discoveries about leprosy indicate that Mycobacterium leprae transmission mainly occurs by inhalation, and the nose is major port of entry and exit. The present study evaluated the clinical application of PCR for detection of M. leprae DNA in nasal mucosa biopsies in untreated leprosy patients (52) and their contacts (99) from the State Reference Center in Sanitary Dermatology and Leprosy, Uberlandia, MG, Brazil. PCR detection of a 372-base pair DNA fragment from M. leprae was accomplished in 36 (69.2%) patients, from which 34 (91.9%) of them were multibacillaries. Furthermore, PCR was positive in 3 (16.7%) of 18 slit-skin smear negative, 4 (25.0%) of 16 skin lesion BI negative, 8 (33.3%) of 24 nasal mucosa BI negative patients, and 10 of 99 contacts (10.1%). The presence of bacilli in 10.1% of the contacts may potentially reflect an occult leprosy, and these patients must be accompanied, followed by a chemoprophylaxy treatment. Considering all PCR results against clinical and BI classification of patients and controls, we have found a sensitivity of 69.2%, a specificity of 89.9%, and an accuracy of 82.8%. It has been demonstrated here through PCR of nasal biopsies that the bacillus invades the mucosa, passing through the nasal inferior turbinate to reach peripheral blood. Therefore, the molecular investigation of invasive nasal biopsies by PCR tests has proven to be useful in defining patients of higher risk of transmission and risk-group contacts, which is an important step to reach the World Health Organization objective towards the elimination of leprosy as a public health problem. SN - 0928-8244 UR - https://www.unboundmedicine.com/medline/citation/15907454/Detection_of_Mycobacterium_leprae_in_nasal_mucosa_biopsies_by_the_polymerase_chain_reaction_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0928-8244&date=2005&volume=44&issue=3&spage=311 DB - PRIME DP - Unbound Medicine ER -