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Transcriptome analysis of Anopheles stephensi-Plasmodium berghei interactions.
Mol Biochem Parasitol. 2005 Jul; 142(1):76-87.MB

Abstract

Simultaneous microarray-based transcription analysis of 4987 Anopheles stephensi midgut and Plasmodium berghei infection stage specific cDNAs was done at seven successive time points: 6, 20 and 40h, and 4, 8, 14 and 20 days after ingestion of malaria infected blood. The study reveals the molecular components of several Anopheles processes relating to blood digestion, midgut expansion and response to Plasmodium-infected blood such as digestive enzymes, transporters, cytoskeletal and structural components and stress and immune responsive factors. In parallel, the analysis provide detailed expression patterns of Plasmodium genes encoding essential developmental and metabolic factors and proteins implicated in interaction with the mosquito vector and vertebrate host such as kinases, transcription and translational factors, cytoskeletal components and a variety of surface proteins, some of which are potent vaccine targets. Temporal correlation between transcription profiles of both organisms identifies putative gene clusters of interacting processes, such as Plasmodium invasion of the midgut epithelium, Anopheles immune responses to Plasmodium infection, and apoptosis and expulsion of invaded midgut cells from the epithelium. Intriguing transcription patterns for highly variable Plasmodium surface antigens may indicate parasite strategies to avoid recognition by the mosquito's immune surveillance system.

Authors+Show Affiliations

Department of Biological Sciences, Imperial College, London SW7 2AZ, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15907562

Citation

Xu, Xiaojin, et al. "Transcriptome Analysis of Anopheles stephensi-Plasmodium Berghei Interactions." Molecular and Biochemical Parasitology, vol. 142, no. 1, 2005, pp. 76-87.
Xu X, Dong Y, Abraham EG, et al. Transcriptome analysis of Anopheles stephensi-Plasmodium berghei interactions. Mol Biochem Parasitol. 2005;142(1):76-87.
Xu, X., Dong, Y., Abraham, E. G., Kocan, A., Srinivasan, P., Ghosh, A. K., Sinden, R. E., Ribeiro, J. M., Jacobs-Lorena, M., Kafatos, F. C., & Dimopoulos, G. (2005). Transcriptome analysis of Anopheles stephensi-Plasmodium berghei interactions. Molecular and Biochemical Parasitology, 142(1), 76-87.
Xu X, et al. Transcriptome Analysis of Anopheles stephensi-Plasmodium Berghei Interactions. Mol Biochem Parasitol. 2005;142(1):76-87. PubMed PMID: 15907562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome analysis of Anopheles stephensi-Plasmodium berghei interactions. AU - Xu,Xiaojin, AU - Dong,Yuemei, AU - Abraham,Eappen G, AU - Kocan,Anna, AU - Srinivasan,Prakash, AU - Ghosh,Anil K, AU - Sinden,Robert E, AU - Ribeiro,Jose M C, AU - Jacobs-Lorena,Marcelo, AU - Kafatos,Fotis C, AU - Dimopoulos,George, Y1 - 2005/04/12/ PY - 2004/12/28/received PY - 2005/02/23/revised PY - 2005/02/25/accepted PY - 2005/5/24/pubmed PY - 2005/9/15/medline PY - 2005/5/24/entrez SP - 76 EP - 87 JF - Molecular and biochemical parasitology JO - Mol. Biochem. Parasitol. VL - 142 IS - 1 N2 - Simultaneous microarray-based transcription analysis of 4987 Anopheles stephensi midgut and Plasmodium berghei infection stage specific cDNAs was done at seven successive time points: 6, 20 and 40h, and 4, 8, 14 and 20 days after ingestion of malaria infected blood. The study reveals the molecular components of several Anopheles processes relating to blood digestion, midgut expansion and response to Plasmodium-infected blood such as digestive enzymes, transporters, cytoskeletal and structural components and stress and immune responsive factors. In parallel, the analysis provide detailed expression patterns of Plasmodium genes encoding essential developmental and metabolic factors and proteins implicated in interaction with the mosquito vector and vertebrate host such as kinases, transcription and translational factors, cytoskeletal components and a variety of surface proteins, some of which are potent vaccine targets. Temporal correlation between transcription profiles of both organisms identifies putative gene clusters of interacting processes, such as Plasmodium invasion of the midgut epithelium, Anopheles immune responses to Plasmodium infection, and apoptosis and expulsion of invaded midgut cells from the epithelium. Intriguing transcription patterns for highly variable Plasmodium surface antigens may indicate parasite strategies to avoid recognition by the mosquito's immune surveillance system. SN - 0166-6851 UR - https://www.unboundmedicine.com/medline/citation/15907562/Transcriptome_analysis_of_Anopheles_stephensi_Plasmodium_berghei_interactions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-6851(05)00107-6 DB - PRIME DP - Unbound Medicine ER -