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Stability-indicating methods for determination of vincamine in presence of its degradation product.
J Pharm Biomed Anal. 2005 Jun 01; 38(1):72-8.JP

Abstract

Three different stability indicating assay methods are developed and validated for determination of vincamine in the presence of its degradation product (vincaminic acid). The first method is based on the derivative ratio zero crossing spectrophotometric technique using 0.1 N hydrochloric acid as a solvent. In the second method, measurements are based on spectro-densitometric technique using high performance thin-layer chromatography (HPTLC) plates with a developing system consisting of methanol-chloroform-ethyl acetate (2:1:1, v/v/v). The third method depends on high-performance liquid chromatography (HPLC). Separation of vincamine from vincaminic acid using Lichrocart RP-18 column (250 mm x 4.6 mm i.d.) with a mobile phase consisting of acetonitrile-ammonium carbonate (0.01 M) (7:3, v/v) is achieved. The methods showed high sensitivity with good linearity over the concentration ranges of 12 to 48 microg ml-1, 3 to 17 microg/spot, and 2 to 20 microg ml-1 for derivative spectrophotometry, spectro-densitometry and HPLC methods, respectively. The developed methods were successfully applied to the analysis of pharmaceutical formulations containing vincamine with excellent recoveries.

Authors+Show Affiliations

Shehata MA
Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasrel Aini Street, Cairo, Egypt ET 11562, Egypt. mostafa1960@yahoo.com
El Sayed MA
No affiliation info available
El Tarras MF
No affiliation info available
El Bardicy MG
No affiliation info available

MeSH

Chromatography, High Pressure LiquidDensitometryDrug StabilityReproducibility of ResultsVincamine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15907622

Citation

Shehata, Mostafa A M., et al. "Stability-indicating Methods for Determination of Vincamine in Presence of Its Degradation Product." Journal of Pharmaceutical and Biomedical Analysis, vol. 38, no. 1, 2005, pp. 72-8.
Shehata MA, El Sayed MA, El Tarras MF, et al. Stability-indicating methods for determination of vincamine in presence of its degradation product. J Pharm Biomed Anal. 2005;38(1):72-8.
Shehata, M. A., El Sayed, M. A., El Tarras, M. F., & El Bardicy, M. G. (2005). Stability-indicating methods for determination of vincamine in presence of its degradation product. Journal of Pharmaceutical and Biomedical Analysis, 38(1), 72-8.
Shehata MA, et al. Stability-indicating Methods for Determination of Vincamine in Presence of Its Degradation Product. J Pharm Biomed Anal. 2005 Jun 1;38(1):72-8. PubMed PMID: 15907622.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stability-indicating methods for determination of vincamine in presence of its degradation product. AU - Shehata,Mostafa A M, AU - El Sayed,Mohammad A, AU - El Tarras,Mohammad F, AU - El Bardicy,Mohammad G, PY - 2003/08/31/received PY - 2004/11/26/revised PY - 2004/11/27/accepted PY - 2005/5/24/pubmed PY - 2005/11/3/medline PY - 2005/5/24/entrez SP - 72 EP - 8 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 38 IS - 1 N2 - Three different stability indicating assay methods are developed and validated for determination of vincamine in the presence of its degradation product (vincaminic acid). The first method is based on the derivative ratio zero crossing spectrophotometric technique using 0.1 N hydrochloric acid as a solvent. In the second method, measurements are based on spectro-densitometric technique using high performance thin-layer chromatography (HPTLC) plates with a developing system consisting of methanol-chloroform-ethyl acetate (2:1:1, v/v/v). The third method depends on high-performance liquid chromatography (HPLC). Separation of vincamine from vincaminic acid using Lichrocart RP-18 column (250 mm x 4.6 mm i.d.) with a mobile phase consisting of acetonitrile-ammonium carbonate (0.01 M) (7:3, v/v) is achieved. The methods showed high sensitivity with good linearity over the concentration ranges of 12 to 48 microg ml-1, 3 to 17 microg/spot, and 2 to 20 microg ml-1 for derivative spectrophotometry, spectro-densitometry and HPLC methods, respectively. The developed methods were successfully applied to the analysis of pharmaceutical formulations containing vincamine with excellent recoveries. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/15907622/Stability_indicating_methods_for_determination_of_vincamine_in_presence_of_its_degradation_product_ DB - PRIME DP - Unbound Medicine ER -

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