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Potential teratogenic effects of benomyl in rat embryos cultured in vitro.
Reprod Toxicol. 2005 Jul-Aug; 20(2):271-80.RT

Abstract

A possible association between environmental exposure to benomyl and anophthalmia has been suggested. The aim of the present study was to investigate potential teratogenic effects of benomyl using the 9.5 day rat embryo culture method using rat and human serum. Explanted rat embryos were cultured in rat serum (n=121) or human serum (n=90) with differing concentrations of benomyl [170 nM to 13.6 microM], dissolved in ethanol (0.136%), at least five embryos per concentration being cultured. In addition, 18 embryos were cultured in both human and rat serum with the equivalent concentration of ethanol to act as a vehicle control. The cultured embryos were then measured and scored for growth and differentiation by two blinded observers. Embryotoxic effects were considered to be demonstrated by a decrease in parameters of growth such as crown rump length, yolk sac diameter and protein content, whereas embryopathic effects were considered to be those causing a decease in parameters of differentiation such as morphological score, somite number and optic development. Benomyl [> or =5 microM] produced a significant concentration dependent deterioration in morphological score, somite number and optic development. Gross toxic effects were noticed at concentrations of >12 microM in rat serum and >10microM in human serum as indicated by a significant effect on parameters measuring size (crown rump length; yolk sac diameter and protein content). This study provides evidence that benomyl is a potential developmental toxicant, affecting many parameters of differentiation, including optic development at levels below those that could be considered embryotoxic.

Authors+Show Affiliations

University of Leeds, Gledhow Wing, St James's University Hospital, Department of Obstetrics and Gynaecology, Level 9, Leeds LS97TF, UK. matt.hewitt@doctors.org.ukNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15907663

Citation

Hewitt, Matthew J., et al. "Potential Teratogenic Effects of Benomyl in Rat Embryos Cultured in Vitro." Reproductive Toxicology (Elmsford, N.Y.), vol. 20, no. 2, 2005, pp. 271-80.
Hewitt MJ, Mutch P, Pratten MK. Potential teratogenic effects of benomyl in rat embryos cultured in vitro. Reprod Toxicol. 2005;20(2):271-80.
Hewitt, M. J., Mutch, P., & Pratten, M. K. (2005). Potential teratogenic effects of benomyl in rat embryos cultured in vitro. Reproductive Toxicology (Elmsford, N.Y.), 20(2), 271-80.
Hewitt MJ, Mutch P, Pratten MK. Potential Teratogenic Effects of Benomyl in Rat Embryos Cultured in Vitro. Reprod Toxicol. 2005 Jul-Aug;20(2):271-80. PubMed PMID: 15907663.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potential teratogenic effects of benomyl in rat embryos cultured in vitro. AU - Hewitt,Matthew J, AU - Mutch,Philip, AU - Pratten,Margaret K, Y1 - 2005/04/18/ PY - 2004/11/04/received PY - 2005/02/08/revised PY - 2005/02/14/accepted PY - 2005/5/24/pubmed PY - 2005/8/17/medline PY - 2005/5/24/entrez SP - 271 EP - 80 JF - Reproductive toxicology (Elmsford, N.Y.) JO - Reprod. Toxicol. VL - 20 IS - 2 N2 - A possible association between environmental exposure to benomyl and anophthalmia has been suggested. The aim of the present study was to investigate potential teratogenic effects of benomyl using the 9.5 day rat embryo culture method using rat and human serum. Explanted rat embryos were cultured in rat serum (n=121) or human serum (n=90) with differing concentrations of benomyl [170 nM to 13.6 microM], dissolved in ethanol (0.136%), at least five embryos per concentration being cultured. In addition, 18 embryos were cultured in both human and rat serum with the equivalent concentration of ethanol to act as a vehicle control. The cultured embryos were then measured and scored for growth and differentiation by two blinded observers. Embryotoxic effects were considered to be demonstrated by a decrease in parameters of growth such as crown rump length, yolk sac diameter and protein content, whereas embryopathic effects were considered to be those causing a decease in parameters of differentiation such as morphological score, somite number and optic development. Benomyl [> or =5 microM] produced a significant concentration dependent deterioration in morphological score, somite number and optic development. Gross toxic effects were noticed at concentrations of >12 microM in rat serum and >10microM in human serum as indicated by a significant effect on parameters measuring size (crown rump length; yolk sac diameter and protein content). This study provides evidence that benomyl is a potential developmental toxicant, affecting many parameters of differentiation, including optic development at levels below those that could be considered embryotoxic. SN - 0890-6238 UR - https://www.unboundmedicine.com/medline/citation/15907663/Potential_teratogenic_effects_of_benomyl_in_rat_embryos_cultured_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0890-6238(05)00069-9 DB - PRIME DP - Unbound Medicine ER -