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Gastroprotective activity of Pradosia huberi on experimentally induced gastric lesions in rodents: role of endogenous sulphydryls and nitric oxide.
J Ethnopharmacol. 2005 Oct 03; 101(1-3):61-7.JE

Abstract

Pradosia huberi is a medicinal plant very common in the Amazonian forest population. The research interest in this plant is justifiable because of its potential medicinal value in gastritis and gastric ulcer mentioned in local folk medicine. In this paper, we evaluated the acute toxicity and antiulcerogenic effect of a hydroalcoholic extract (HAE) obtained from Pradosia huberi barks in rodents. No acute toxicological sign or symptom was observed in animals treated with the highest dose (5000 mg/kg, p.o.) of Pradosia huberi. In the HCl/EtOH-induced gastric ulcer model, HAE demonstrated significant inhibition of the ulcerative lesion index by 73% (500 mg/kg) and 88% (1000 mg/kg), respectively, in relation to the control value (p<0.05). The gastric damage induced by absolute ethanol in rats was effectively reduced by 84, 88 and 81% (250, 500 and 1000 mg/kg) when compared with the control group (p<0.01). In the NSAID-induced lesion model, HAE also showed antiulcerogenic effect with decrease in gastric lesions of 56% (250 mg/kg), 57% (500 mg/kg) and 67 % (1000 mg/kg) when compared with animals treated with vehicle (p<0.05). In the gastric ulcer induced by pylorus ligature model, the administration of HAE by oral and intraduodenal routes inhibited the gastric lesion index by 79 and 52% (500 mg/kg), respectively. HAE administered orally or intraduodenally was able to change gastric juice parameters (pH, volume and acid output) as well as those treated with cimetidine. The treatment with HAE (p.o.) significantly increased gastric volume, the pH values and promoted reduced acid output (p<0.01). By comparative effect (intraduodenal and oral route), we observed that HAE was better for local activity in gastric mucosa than in systemic action. HAE also has a non-specific activity when found to be the inhibitor of intestinal motility (p>0.01). The mechanism of action of HAE did not seem to be related to the NO-inhibitor but showed the participation of endogenous sulphydryl group in the gastroprotective action.

Authors+Show Affiliations

Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, CP 510, CEP 18608-000, Botucatu, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15908153

Citation

Kushima, Hélio, et al. "Gastroprotective Activity of Pradosia Huberi On Experimentally Induced Gastric Lesions in Rodents: Role of Endogenous Sulphydryls and Nitric Oxide." Journal of Ethnopharmacology, vol. 101, no. 1-3, 2005, pp. 61-7.
Kushima H, Hiruma-Lima CA, Santos MA, et al. Gastroprotective activity of Pradosia huberi on experimentally induced gastric lesions in rodents: role of endogenous sulphydryls and nitric oxide. J Ethnopharmacol. 2005;101(1-3):61-7.
Kushima, H., Hiruma-Lima, C. A., Santos, M. A., Viana, E., Coelho-Ferreira, M., & Brito, A. R. (2005). Gastroprotective activity of Pradosia huberi on experimentally induced gastric lesions in rodents: role of endogenous sulphydryls and nitric oxide. Journal of Ethnopharmacology, 101(1-3), 61-7.
Kushima H, et al. Gastroprotective Activity of Pradosia Huberi On Experimentally Induced Gastric Lesions in Rodents: Role of Endogenous Sulphydryls and Nitric Oxide. J Ethnopharmacol. 2005 Oct 3;101(1-3):61-7. PubMed PMID: 15908153.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gastroprotective activity of Pradosia huberi on experimentally induced gastric lesions in rodents: role of endogenous sulphydryls and nitric oxide. AU - Kushima,Hélio, AU - Hiruma-Lima,Clélia Akiko, AU - Santos,Maria Aparecida, AU - Viana,Elizabeth, AU - Coelho-Ferreira,Márlia, AU - Brito,Alba Regina Monteiro Souza, PY - 2004/10/08/received PY - 2005/03/13/revised PY - 2005/03/24/accepted PY - 2005/5/24/pubmed PY - 2005/12/29/medline PY - 2005/5/24/entrez SP - 61 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 101 IS - 1-3 N2 - Pradosia huberi is a medicinal plant very common in the Amazonian forest population. The research interest in this plant is justifiable because of its potential medicinal value in gastritis and gastric ulcer mentioned in local folk medicine. In this paper, we evaluated the acute toxicity and antiulcerogenic effect of a hydroalcoholic extract (HAE) obtained from Pradosia huberi barks in rodents. No acute toxicological sign or symptom was observed in animals treated with the highest dose (5000 mg/kg, p.o.) of Pradosia huberi. In the HCl/EtOH-induced gastric ulcer model, HAE demonstrated significant inhibition of the ulcerative lesion index by 73% (500 mg/kg) and 88% (1000 mg/kg), respectively, in relation to the control value (p<0.05). The gastric damage induced by absolute ethanol in rats was effectively reduced by 84, 88 and 81% (250, 500 and 1000 mg/kg) when compared with the control group (p<0.01). In the NSAID-induced lesion model, HAE also showed antiulcerogenic effect with decrease in gastric lesions of 56% (250 mg/kg), 57% (500 mg/kg) and 67 % (1000 mg/kg) when compared with animals treated with vehicle (p<0.05). In the gastric ulcer induced by pylorus ligature model, the administration of HAE by oral and intraduodenal routes inhibited the gastric lesion index by 79 and 52% (500 mg/kg), respectively. HAE administered orally or intraduodenally was able to change gastric juice parameters (pH, volume and acid output) as well as those treated with cimetidine. The treatment with HAE (p.o.) significantly increased gastric volume, the pH values and promoted reduced acid output (p<0.01). By comparative effect (intraduodenal and oral route), we observed that HAE was better for local activity in gastric mucosa than in systemic action. HAE also has a non-specific activity when found to be the inhibitor of intestinal motility (p>0.01). The mechanism of action of HAE did not seem to be related to the NO-inhibitor but showed the participation of endogenous sulphydryl group in the gastroprotective action. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/15908153/Gastroprotective_activity_of_Pradosia_huberi_on_experimentally_induced_gastric_lesions_in_rodents:_role_of_endogenous_sulphydryls_and_nitric_oxide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(05)00251-5 DB - PRIME DP - Unbound Medicine ER -