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Panax notoginseng saponins preconditioning protects rat liver grafts from ischemia/reperfusion injury via an antiapoptotic pathway.
Hepatobiliary Pancreat Dis Int. 2005 May; 4(2):207-12.HP

Abstract

BACKGROUND

Ischemia/reperfusion (I/R) injury is a major cause of primary graft dysfunction and renders an allograft more immunogenic in orthotopic liver transplantation (OLT). Panax notoginseng saponins (PNS) has been reported to exert protective effects against I/R injury to various organs. The objective of this study is to investigate whether PNS preconditioning protects rat liver grafts from I/R injury via an antiapoptotic pathway.

METHODS

Male Sprague-Dawley rats were used as donors and recipients of orthotopic liver transplantation (OLT) and were divided into PNS preconditioning group(group P) and normal saline control group (group N) randomly according to whether PNS (50 mg/kg) was injected intravenously 1 hour before liver grafts harvesting, and sham group (group S). The animals were separately killed 2, 6 and 24 hours after reperfusion. Plasma samples were collected for test of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver tissues were collected to detect histological changes, apoptosis and the expression of TNF-alpha, Bcl-2 and Caspase-3 mRNA.

RESULTS

The serum levels of ALT and AST and the apoptosis index (AI) of liver tissue in group P were lower than in group N significantly 2, 6 and 24 hours after reperfusion. Compared with group N, the expression of TNF-alpha and Caspase-3 mRNA was reduced significantly in group P 2 and 6 hours after reperfusion and the expression of Bcl-2 mRNA was enhanced significantly in group P 6 and 24 hours after reperfusion.

CONCLUSIONS

PNS preconditioning protects liver grafts from I/R injury effectively in rat OLT via an antiapoptotic pathway. The antiapoptotic mechanisms of PNS may include inhibiting the expression of TNF-alpha and Caspase-3 and enhancing the expression of Bcl-2.

Authors+Show Affiliations

Zhang Y
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Ye QF
No affiliation info available
Lu L
No affiliation info available
Xu XL
No affiliation info available
Ming YZ
No affiliation info available
Xiao JS
No affiliation info available

MeSH

Analysis of VarianceAnimalsApoptosisBase SequenceBiopsy, NeedleDisease Models, AnimalGraft RejectionGraft SurvivalImmunohistochemistryIschemic PreconditioningLiver Function TestsLiver TransplantationMaleMolecular Sequence DataPanaxProbabilityRNA, MessengerRandom AllocationRatsRats, Sprague-DawleyReperfusion InjuryReverse Transcriptase Polymerase Chain ReactionSaponinsSensitivity and Specificity

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15908317

Citation

Zhang, Yi, et al. "Panax Notoginseng Saponins Preconditioning Protects Rat Liver Grafts From Ischemia/reperfusion Injury Via an Antiapoptotic Pathway." Hepatobiliary & Pancreatic Diseases International : HBPD INT, vol. 4, no. 2, 2005, pp. 207-12.
Zhang Y, Ye QF, Lu L, et al. Panax notoginseng saponins preconditioning protects rat liver grafts from ischemia/reperfusion injury via an antiapoptotic pathway. Hepatobiliary Pancreat Dis Int. 2005;4(2):207-12.
Zhang, Y., Ye, Q. F., Lu, L., Xu, X. L., Ming, Y. Z., & Xiao, J. S. (2005). Panax notoginseng saponins preconditioning protects rat liver grafts from ischemia/reperfusion injury via an antiapoptotic pathway. Hepatobiliary & Pancreatic Diseases International : HBPD INT, 4(2), 207-12.
Zhang Y, et al. Panax Notoginseng Saponins Preconditioning Protects Rat Liver Grafts From Ischemia/reperfusion Injury Via an Antiapoptotic Pathway. Hepatobiliary Pancreat Dis Int. 2005;4(2):207-12. PubMed PMID: 15908317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Panax notoginseng saponins preconditioning protects rat liver grafts from ischemia/reperfusion injury via an antiapoptotic pathway. AU - Zhang,Yi, AU - Ye,Qi-Fa, AU - Lu,Li, AU - Xu,Xian-Lin, AU - Ming,Ying-Zi, AU - Xiao,Jian-Sheng, PY - 2005/5/24/pubmed PY - 2005/7/6/medline PY - 2005/5/24/entrez SP - 207 EP - 12 JF - Hepatobiliary & pancreatic diseases international : HBPD INT JO - Hepatobiliary Pancreat Dis Int VL - 4 IS - 2 N2 - BACKGROUND: Ischemia/reperfusion (I/R) injury is a major cause of primary graft dysfunction and renders an allograft more immunogenic in orthotopic liver transplantation (OLT). Panax notoginseng saponins (PNS) has been reported to exert protective effects against I/R injury to various organs. The objective of this study is to investigate whether PNS preconditioning protects rat liver grafts from I/R injury via an antiapoptotic pathway. METHODS: Male Sprague-Dawley rats were used as donors and recipients of orthotopic liver transplantation (OLT) and were divided into PNS preconditioning group(group P) and normal saline control group (group N) randomly according to whether PNS (50 mg/kg) was injected intravenously 1 hour before liver grafts harvesting, and sham group (group S). The animals were separately killed 2, 6 and 24 hours after reperfusion. Plasma samples were collected for test of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver tissues were collected to detect histological changes, apoptosis and the expression of TNF-alpha, Bcl-2 and Caspase-3 mRNA. RESULTS: The serum levels of ALT and AST and the apoptosis index (AI) of liver tissue in group P were lower than in group N significantly 2, 6 and 24 hours after reperfusion. Compared with group N, the expression of TNF-alpha and Caspase-3 mRNA was reduced significantly in group P 2 and 6 hours after reperfusion and the expression of Bcl-2 mRNA was enhanced significantly in group P 6 and 24 hours after reperfusion. CONCLUSIONS: PNS preconditioning protects liver grafts from I/R injury effectively in rat OLT via an antiapoptotic pathway. The antiapoptotic mechanisms of PNS may include inhibiting the expression of TNF-alpha and Caspase-3 and enhancing the expression of Bcl-2. SN - 1499-3872 UR - https://www.unboundmedicine.com/medline/citation/15908317/Panax_notoginseng_saponins_preconditioning_protects_rat_liver_grafts_from_ischemia/reperfusion_injury_via_an_antiapoptotic_pathway_ DB - PRIME DP - Unbound Medicine ER -