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Evaluation of the direct acridine orange staining method and Q.B.C. test for diagnosis of malaria in Delhi, India.
J Commun Dis. 2003 Dec; 35(4):279-82.JC

Abstract

Conventional Giemsa stained peripheral blood smear examination for demonstration of malarial parasites remains the gold standard for diagnosis of malaria in developing endemic countries. However this technique is time consuming, requires training and may give poor results in cases with low parasitaemia. To overcome these problems and improve diagnostic accuracy two newer tests have been studied and compared with standard Giemsa staining. These are the wet mount fluorescence microscopy of Acridine Orange stained thin blood films (A.O.) and the Quantitative Buffy Coat technique (Q.B.C) for diagnosis of malaria. A.O. staining was found to be 97.5% sensitive and 100% specific for detection of all stages and species of malarial parasite. The Q.B.C assay was found to be 100% sensitive and 97.5% specific for diagnosis of malaria. A.O. staining was very fast and the species identification was easy once the staining was optimised. The Q.B.C. test required considerable amount of practice, costly equipment, however it was fast and in our study was found to be highly sensitive.

Authors+Show Affiliations

Dept of Microbiology, University College of Medical Sciences, Shahadra, Delhi.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

15909757

Citation

Nandwani, S, et al. "Evaluation of the Direct Acridine Orange Staining Method and Q.B.C. Test for Diagnosis of Malaria in Delhi, India." The Journal of Communicable Diseases, vol. 35, no. 4, 2003, pp. 279-82.
Nandwani S, Mathur M, Rawat S. Evaluation of the direct acridine orange staining method and Q.B.C. test for diagnosis of malaria in Delhi, India. J Commun Dis. 2003;35(4):279-82.
Nandwani, S., Mathur, M., & Rawat, S. (2003). Evaluation of the direct acridine orange staining method and Q.B.C. test for diagnosis of malaria in Delhi, India. The Journal of Communicable Diseases, 35(4), 279-82.
Nandwani S, Mathur M, Rawat S. Evaluation of the Direct Acridine Orange Staining Method and Q.B.C. Test for Diagnosis of Malaria in Delhi, India. J Commun Dis. 2003;35(4):279-82. PubMed PMID: 15909757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the direct acridine orange staining method and Q.B.C. test for diagnosis of malaria in Delhi, India. AU - Nandwani,S, AU - Mathur,M, AU - Rawat,S, PY - 2005/5/25/pubmed PY - 2005/6/3/medline PY - 2005/5/25/entrez SP - 279 EP - 82 JF - The Journal of communicable diseases JO - J Commun Dis VL - 35 IS - 4 N2 - Conventional Giemsa stained peripheral blood smear examination for demonstration of malarial parasites remains the gold standard for diagnosis of malaria in developing endemic countries. However this technique is time consuming, requires training and may give poor results in cases with low parasitaemia. To overcome these problems and improve diagnostic accuracy two newer tests have been studied and compared with standard Giemsa staining. These are the wet mount fluorescence microscopy of Acridine Orange stained thin blood films (A.O.) and the Quantitative Buffy Coat technique (Q.B.C) for diagnosis of malaria. A.O. staining was found to be 97.5% sensitive and 100% specific for detection of all stages and species of malarial parasite. The Q.B.C assay was found to be 100% sensitive and 97.5% specific for diagnosis of malaria. A.O. staining was very fast and the species identification was easy once the staining was optimised. The Q.B.C. test required considerable amount of practice, costly equipment, however it was fast and in our study was found to be highly sensitive. SN - 0019-5138 UR - https://www.unboundmedicine.com/medline/citation/15909757/Evaluation_of_the_direct_acridine_orange_staining_method_and_Q_B_C__test_for_diagnosis_of_malaria_in_Delhi_India_ DB - PRIME DP - Unbound Medicine ER -