Tags

Type your tag names separated by a space and hit enter

HIV-1 viral proteins gp120 and Tat induce oxidative stress in brain endothelial cells.
Brain Res 2005; 1045(1-2):57-63BR

Abstract

The blood-brain barrier (BBB) has an important role in the development of AIDS dementia. The HIV-1 envelope glycoprotein (gp120) and transregulatory protein (Tat) of HIV-1 are neurotoxic and cytotoxic and have been implicated in the development of HIV dementia. They are known to cause oxidative stress and are associated with disruption of the BBB. Here, we used an immortalized endothelial cell line from rat brain capillaries, RBE4, to determine whether gp120 and Tat can induce oxidative stress in an in vitro model of the BBB. RBE4 cells were exposed to gp120 or Tat and the levels of reduced glutathione (GSH), oxidized glutathione (GSSG), catalase (CAT) activity, glutathione peroxidase (GPx) activity, and glutathione reductase (GR) activity, and malondialdehyde (MDA) used as measures of oxidative stress. Both gp120 and Tat significantly decreased the levels of intracellular GSH, GPx, and GR and increased the levels of MDA in RBE4 cells, showing that the cells were oxidatively challenged. The ratio of GSH/GSSG, a widely accepted indicator of oxidative stress, was also significantly decreased. These studies show that both of these viral proteins can induce oxidative stress in immortalized BBB endothelial cells.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Pharmacy, Marmara University, Istanbul 81010, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15910762

Citation

Price, Tulin Otamis, et al. "HIV-1 Viral Proteins Gp120 and Tat Induce Oxidative Stress in Brain Endothelial Cells." Brain Research, vol. 1045, no. 1-2, 2005, pp. 57-63.
Price TO, Ercal N, Nakaoke R, et al. HIV-1 viral proteins gp120 and Tat induce oxidative stress in brain endothelial cells. Brain Res. 2005;1045(1-2):57-63.
Price, T. O., Ercal, N., Nakaoke, R., & Banks, W. A. (2005). HIV-1 viral proteins gp120 and Tat induce oxidative stress in brain endothelial cells. Brain Research, 1045(1-2), pp. 57-63.
Price TO, et al. HIV-1 Viral Proteins Gp120 and Tat Induce Oxidative Stress in Brain Endothelial Cells. Brain Res. 2005 May 31;1045(1-2):57-63. PubMed PMID: 15910762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HIV-1 viral proteins gp120 and Tat induce oxidative stress in brain endothelial cells. AU - Price,Tulin Otamis, AU - Ercal,Nuran, AU - Nakaoke,Ryota, AU - Banks,William A, Y1 - 2005/04/19/ PY - 2004/12/08/received PY - 2005/03/09/revised PY - 2005/03/15/accepted PY - 2005/5/25/pubmed PY - 2005/8/19/medline PY - 2005/5/25/entrez SP - 57 EP - 63 JF - Brain research JO - Brain Res. VL - 1045 IS - 1-2 N2 - The blood-brain barrier (BBB) has an important role in the development of AIDS dementia. The HIV-1 envelope glycoprotein (gp120) and transregulatory protein (Tat) of HIV-1 are neurotoxic and cytotoxic and have been implicated in the development of HIV dementia. They are known to cause oxidative stress and are associated with disruption of the BBB. Here, we used an immortalized endothelial cell line from rat brain capillaries, RBE4, to determine whether gp120 and Tat can induce oxidative stress in an in vitro model of the BBB. RBE4 cells were exposed to gp120 or Tat and the levels of reduced glutathione (GSH), oxidized glutathione (GSSG), catalase (CAT) activity, glutathione peroxidase (GPx) activity, and glutathione reductase (GR) activity, and malondialdehyde (MDA) used as measures of oxidative stress. Both gp120 and Tat significantly decreased the levels of intracellular GSH, GPx, and GR and increased the levels of MDA in RBE4 cells, showing that the cells were oxidatively challenged. The ratio of GSH/GSSG, a widely accepted indicator of oxidative stress, was also significantly decreased. These studies show that both of these viral proteins can induce oxidative stress in immortalized BBB endothelial cells. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/15910762/HIV_1_viral_proteins_gp120_and_Tat_induce_oxidative_stress_in_brain_endothelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(05)00460-9 DB - PRIME DP - Unbound Medicine ER -