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Toward a detailed characterization of feline immunodeficiency virus-specific T cell immune responses and mediated immune disorders.
Vet Immunol Immunopathol. 2005 Jun 15; 106(1-2):1-14.VI

Abstract

Infection of domestic cats with feline immunodeficiency virus (FIV) is associated with the development of an acquired immunodeficiency syndrome (AIDS). The pathogenesis of FIV is not fully understood but it has been reported that the immune system is progressively impaired during disease progression. As a result, anti-FIV specific immune response will usually not clear the virus and the acute stage is followed by a chronic asymptomatic phase. The overall objective of this study was to characterized FIV-induced immune cellular responses and -mediated immune disorder following the first weeks post-infection. Using both cytokine ELISpot and intracellular staining assays, FIV-specific T cells were monitored at 6, 9 and 12 weeks post-infection. We demonstrated that both IFNgamma(+) and, CD4 and CD8 TNFalpha(+) T cells specifically respond to FIV antigens. These responses were found to reach a peak at 9 weeks post-infection. It was further shown that the TNFalpha(+)CD8(+) responding T cells were contained within a CD8beta(low)CD62L(-) T cell subpopulation, expanded in FIV-infected cats. This T cell subpopulation which present features of activated CD8 T cells was further shown to be susceptible to spontaneous apoptosis following a short-term in vitro culture. Moreover, it was observed that cell death by apoptosis of this T cell subset was increased following FIV antigen-recognition. Therefore, FIV might alter immune homeostasis in inducing chronic activation of TNFalpha(+)CD8(+) T cells which eventually will die following antigen contact while deleting CD4(+) T cells. Interestingly, this study confirmed the strong similarity between FIV and HIV pathogenesis.

Authors+Show Affiliations

Paillot R
Discovery Research, Merial S.A.S., 254 rue Marcel Mérieux BP 7009, 69342 Lyon Cedex 07, France.
Richard S
No affiliation info available
Bloas F
No affiliation info available
Piras F
No affiliation info available
Poulet H
No affiliation info available
Brunet S
No affiliation info available
Andreoni C
No affiliation info available
Juillard V
No affiliation info available

MeSH

AnimalsAntigens, ViralApoptosisCat DiseasesCatsFemaleImmunodeficiency Virus, FelineInterferon-gammaLentivirus InfectionsLymphocyte ActivationMaleSpecific Pathogen-Free OrganismsT-Lymphocyte SubsetsTumor Necrosis Factor-alphaViremia

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15910988

Citation

Paillot, R, et al. "Toward a Detailed Characterization of Feline Immunodeficiency Virus-specific T Cell Immune Responses and Mediated Immune Disorders." Veterinary Immunology and Immunopathology, vol. 106, no. 1-2, 2005, pp. 1-14.
Paillot R, Richard S, Bloas F, et al. Toward a detailed characterization of feline immunodeficiency virus-specific T cell immune responses and mediated immune disorders. Vet Immunol Immunopathol. 2005;106(1-2):1-14.
Paillot, R., Richard, S., Bloas, F., Piras, F., Poulet, H., Brunet, S., Andreoni, C., & Juillard, V. (2005). Toward a detailed characterization of feline immunodeficiency virus-specific T cell immune responses and mediated immune disorders. Veterinary Immunology and Immunopathology, 106(1-2), 1-14.
Paillot R, et al. Toward a Detailed Characterization of Feline Immunodeficiency Virus-specific T Cell Immune Responses and Mediated Immune Disorders. Vet Immunol Immunopathol. 2005 Jun 15;106(1-2):1-14. PubMed PMID: 15910988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toward a detailed characterization of feline immunodeficiency virus-specific T cell immune responses and mediated immune disorders. AU - Paillot,R, AU - Richard,S, AU - Bloas,F, AU - Piras,F, AU - Poulet,H, AU - Brunet,S, AU - Andreoni,C, AU - Juillard,V, PY - 2004/03/22/received PY - 2004/11/29/revised PY - 2004/12/21/accepted PY - 2005/5/25/pubmed PY - 2005/8/10/medline PY - 2005/5/25/entrez SP - 1 EP - 14 JF - Veterinary immunology and immunopathology JO - Vet Immunol Immunopathol VL - 106 IS - 1-2 N2 - Infection of domestic cats with feline immunodeficiency virus (FIV) is associated with the development of an acquired immunodeficiency syndrome (AIDS). The pathogenesis of FIV is not fully understood but it has been reported that the immune system is progressively impaired during disease progression. As a result, anti-FIV specific immune response will usually not clear the virus and the acute stage is followed by a chronic asymptomatic phase. The overall objective of this study was to characterized FIV-induced immune cellular responses and -mediated immune disorder following the first weeks post-infection. Using both cytokine ELISpot and intracellular staining assays, FIV-specific T cells were monitored at 6, 9 and 12 weeks post-infection. We demonstrated that both IFNgamma(+) and, CD4 and CD8 TNFalpha(+) T cells specifically respond to FIV antigens. These responses were found to reach a peak at 9 weeks post-infection. It was further shown that the TNFalpha(+)CD8(+) responding T cells were contained within a CD8beta(low)CD62L(-) T cell subpopulation, expanded in FIV-infected cats. This T cell subpopulation which present features of activated CD8 T cells was further shown to be susceptible to spontaneous apoptosis following a short-term in vitro culture. Moreover, it was observed that cell death by apoptosis of this T cell subset was increased following FIV antigen-recognition. Therefore, FIV might alter immune homeostasis in inducing chronic activation of TNFalpha(+)CD8(+) T cells which eventually will die following antigen contact while deleting CD4(+) T cells. Interestingly, this study confirmed the strong similarity between FIV and HIV pathogenesis. SN - 0165-2427 UR - https://www.unboundmedicine.com/medline/citation/15910988/Toward_a_detailed_characterization_of_feline_immunodeficiency_virus_specific_T_cell_immune_responses_and_mediated_immune_disorders_ DB - PRIME DP - Unbound Medicine ER -