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Serological markers during dengue 3 primary and secondary infections.
J Clin Virol. 2005 Jun; 33(2):132-7.JC

Abstract

BACKGROUND

The detection of the IgM antibody for the dengue virus in serum by ELISA has become one of the most important and useful methods for diagnosis of dengue using a single acute-phase serum sample. Currently, this system is an invaluable tool for the surveillance of dengue fever (DF) and dengue hemorrhagic fever (DHF). The usefulness of other serological markers such as IgA and IgE have been less studied.

OBJECTIVE

To study the IgM, IgA and IgE specific antibody response in dengue 3 infected patients with different clinical picture and type of infection.

STUDY DESIGN

One hundred and twenty-seven serum samples collected on days 5-7 at the onset of fever from clinically and serologically confirmed dengue cases were studied. Forty-two were classified as primary dengue fever cases, 48 as secondary dengue fever cases and 37 as secondary dengue hemorrhagic fever cases. All samples were tested by capture ELISA in order to detect dengue IgM, IgA and IgE antibodies.

RESULTS AND CONCLUSIONS

In this study, significant differences were observed in the IgM, IgA and IgE response between the study groups. High IgA and IgE OD ratios in secondary dengue cases were found. The usefulness of serotype specific IgM antibody detection is also analyzed and discussed. A priority for future dengue research in terms of protection, recovery of infection and immunopathogenesis is to elucidate the role of these immunoglobulins. The cross reactivity response to IgM between dengue virus serotypes in primary and secondary cases should also be more studied.

Authors+Show Affiliations

Virology Department, PAHO/WHO Collaborating Center for Viral Diseases, Pedro Kourí Institute, Autopista Novia del Mediodía, Km 61/2, La Lisa, Ciudad de la Habana, P.O. Box: 601 Marianao 13, Havana City, Cuba (IPK). svazquez@ipk.sld.cuNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15911428

Citation

Vázquez, S, et al. "Serological Markers During Dengue 3 Primary and Secondary Infections." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 33, no. 2, 2005, pp. 132-7.
Vázquez S, Pérez AB, Ruiz D, et al. Serological markers during dengue 3 primary and secondary infections. J Clin Virol. 2005;33(2):132-7.
Vázquez, S., Pérez, A. B., Ruiz, D., Rodríguez, R., Pupo, M., Calzada, N., González, L., González, D., Castro, O., Serrano, T., & Guzmán, M. G. (2005). Serological markers during dengue 3 primary and secondary infections. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 33(2), 132-7.
Vázquez S, et al. Serological Markers During Dengue 3 Primary and Secondary Infections. J Clin Virol. 2005;33(2):132-7. PubMed PMID: 15911428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serological markers during dengue 3 primary and secondary infections. AU - Vázquez,S, AU - Pérez,A B, AU - Ruiz,D, AU - Rodríguez,R, AU - Pupo,M, AU - Calzada,N, AU - González,L, AU - González,D, AU - Castro,O, AU - Serrano,T, AU - Guzmán,M G, Y1 - 2004/12/18/ PY - 2004/07/06/received PY - 2004/08/20/revised PY - 2004/10/14/accepted PY - 2005/5/25/pubmed PY - 2005/10/4/medline PY - 2005/5/25/entrez SP - 132 EP - 7 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 33 IS - 2 N2 - BACKGROUND: The detection of the IgM antibody for the dengue virus in serum by ELISA has become one of the most important and useful methods for diagnosis of dengue using a single acute-phase serum sample. Currently, this system is an invaluable tool for the surveillance of dengue fever (DF) and dengue hemorrhagic fever (DHF). The usefulness of other serological markers such as IgA and IgE have been less studied. OBJECTIVE: To study the IgM, IgA and IgE specific antibody response in dengue 3 infected patients with different clinical picture and type of infection. STUDY DESIGN: One hundred and twenty-seven serum samples collected on days 5-7 at the onset of fever from clinically and serologically confirmed dengue cases were studied. Forty-two were classified as primary dengue fever cases, 48 as secondary dengue fever cases and 37 as secondary dengue hemorrhagic fever cases. All samples were tested by capture ELISA in order to detect dengue IgM, IgA and IgE antibodies. RESULTS AND CONCLUSIONS: In this study, significant differences were observed in the IgM, IgA and IgE response between the study groups. High IgA and IgE OD ratios in secondary dengue cases were found. The usefulness of serotype specific IgM antibody detection is also analyzed and discussed. A priority for future dengue research in terms of protection, recovery of infection and immunopathogenesis is to elucidate the role of these immunoglobulins. The cross reactivity response to IgM between dengue virus serotypes in primary and secondary cases should also be more studied. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/15911428/Serological_markers_during_dengue_3_primary_and_secondary_infections_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(04)00298-7 DB - PRIME DP - Unbound Medicine ER -