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Divergent genetic evolution of hemagglutinin in influenza A H1N1 and A H1N2 subtypes isolated in the south-France since the winter of 2001-2002.
J Clin Virol. 2005 Jul; 33(3):230-6.JC

Abstract

BACKGROUND

Influenza A viruses are divided into subtypes based on their hemagglutinin (H1 to H15) and neuraminidase (N1 to N9) glycoproteins. Of these, three A subtypes H1N1, H3N2 and H1N2 circulate in the human population. Influenza A viruses display a high antigenic variability called "antigenic drift" which allows the virus to escape antibody neutralization.

OBJECTIVES

Evaluate the mutations apparition that might predict a divergent antigenic evolution of hemagglutinin in influenza A H1N1 and A H1N2 viruses.

STUDY DESIGN

During the three winters of 2001-2002 to 2003-2004, 58 A H1N1 and 23 A H1N2 subtypes have been isolated from patients with influenza-like illness in the south of France. The HA1 region was analyzed by RT-PCR and subsequently sequenced to compare the HA1 genetic evolution of influenza A H1N1 and A H1N2 subtypes.

RESULTS

Our results showed that 28 amino acid substitutions have accumulated in the HA1 region since the circulation of A/New Caledonia/20/99-like viruses in France. Of these, fifteen were located in four antigenic sites (B, C, D and E). Six of them were observed only in the A H1N2 isolates, six only in the A H1N1 isolates and three in both subtypes. Furthermore, nine of twenty two A H1N2 isolates from the winter of 2002-2003 shared a T90A amino acid change which has not been observed in any A H1N1 isolate; resulting in the introduction of a new glycosylation site close to the antigenic site E. This might mask some antigenic E determinants and therefore, modify the A H1N2 antigenicity.

CONCLUSIONS

The divergent genetic evolution of hemagglutinin may ultimately lead to a significant different antigenicity between A H1N1 and A H1N2 subtypes that would require the introduction of a new subtype in the vaccine batches.

Authors+Show Affiliations

Unite de Virologie Medicale, Laboratoire de Virologie, UMR CNRS 5537, CNR des Virus Influenza Région Sud, Domaine Rockefeller, 69373 Lyon Cedex 08, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15911445

Citation

Al Faress, Shaker, et al. "Divergent Genetic Evolution of Hemagglutinin in Influenza a H1N1 and a H1N2 Subtypes Isolated in the south-France Since the Winter of 2001-2002." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 33, no. 3, 2005, pp. 230-6.
Al Faress S, Cartet G, Ferraris O, et al. Divergent genetic evolution of hemagglutinin in influenza A H1N1 and A H1N2 subtypes isolated in the south-France since the winter of 2001-2002. J Clin Virol. 2005;33(3):230-6.
Al Faress, S., Cartet, G., Ferraris, O., Norder, H., Valette, M., & Lina, B. (2005). Divergent genetic evolution of hemagglutinin in influenza A H1N1 and A H1N2 subtypes isolated in the south-France since the winter of 2001-2002. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 33(3), 230-6.
Al Faress S, et al. Divergent Genetic Evolution of Hemagglutinin in Influenza a H1N1 and a H1N2 Subtypes Isolated in the south-France Since the Winter of 2001-2002. J Clin Virol. 2005;33(3):230-6. PubMed PMID: 15911445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Divergent genetic evolution of hemagglutinin in influenza A H1N1 and A H1N2 subtypes isolated in the south-France since the winter of 2001-2002. AU - Al Faress,Shaker, AU - Cartet,Gaëlle, AU - Ferraris,Olivier, AU - Norder,Helene, AU - Valette,Martine, AU - Lina,Bruno, Y1 - 2005/01/19/ PY - 2004/10/20/received PY - 2004/11/19/revised PY - 2004/11/22/accepted PY - 2005/5/25/pubmed PY - 2005/7/27/medline PY - 2005/5/25/entrez SP - 230 EP - 6 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J. Clin. Virol. VL - 33 IS - 3 N2 - BACKGROUND: Influenza A viruses are divided into subtypes based on their hemagglutinin (H1 to H15) and neuraminidase (N1 to N9) glycoproteins. Of these, three A subtypes H1N1, H3N2 and H1N2 circulate in the human population. Influenza A viruses display a high antigenic variability called "antigenic drift" which allows the virus to escape antibody neutralization. OBJECTIVES: Evaluate the mutations apparition that might predict a divergent antigenic evolution of hemagglutinin in influenza A H1N1 and A H1N2 viruses. STUDY DESIGN: During the three winters of 2001-2002 to 2003-2004, 58 A H1N1 and 23 A H1N2 subtypes have been isolated from patients with influenza-like illness in the south of France. The HA1 region was analyzed by RT-PCR and subsequently sequenced to compare the HA1 genetic evolution of influenza A H1N1 and A H1N2 subtypes. RESULTS: Our results showed that 28 amino acid substitutions have accumulated in the HA1 region since the circulation of A/New Caledonia/20/99-like viruses in France. Of these, fifteen were located in four antigenic sites (B, C, D and E). Six of them were observed only in the A H1N2 isolates, six only in the A H1N1 isolates and three in both subtypes. Furthermore, nine of twenty two A H1N2 isolates from the winter of 2002-2003 shared a T90A amino acid change which has not been observed in any A H1N1 isolate; resulting in the introduction of a new glycosylation site close to the antigenic site E. This might mask some antigenic E determinants and therefore, modify the A H1N2 antigenicity. CONCLUSIONS: The divergent genetic evolution of hemagglutinin may ultimately lead to a significant different antigenicity between A H1N1 and A H1N2 subtypes that would require the introduction of a new subtype in the vaccine batches. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/15911445/Divergent_genetic_evolution_of_hemagglutinin_in_influenza_A_H1N1_and_A_H1N2_subtypes_isolated_in_the_south_France_since_the_winter_of_2001_2002_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(04)00345-2 DB - PRIME DP - Unbound Medicine ER -