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Characterization of tryptophan-overproducing potato transgenic for a mutant rice anthranilate synthase alpha-subunit gene (OASA1D).
Planta. 2005 Oct; 222(3):535-45.P

Abstract

Potato plants (Solanum tuberosum cv. May Queen) transgenic for OASA1D, which encodes a point mutant of an alpha-subunit of rice (Oryza sativa) anthranilate synthase (AS, EC 4.1.3.27), were generated in order to determine the effects of the mutant gene on levels of free tryptophan (Trp) and AS activity in this important crop. Expression of OASA1D in potato induced a 2- to 20-fold increase in the amount of free Trp. This increase was likely due to a reduction in the sensitivity of AS containing the mutant alpha-subunit to feedback inhibition by Trp. Nontargeted metabolite profiling by high-performance liquid chromatography coupled with ultraviolet photodiode array detection as well as targeted profiling by liquid chromatography coupled with mass spectrometry revealed no marked changes in the levels of other metabolites, with the exception of indole-3-acetic acid (IAA), in the transgenic plants. The level of IAA in the upper part of the shoot was increased by a factor of 8.3-39, depending on the transgenic lines, with no detectable effect on plant growth or development. The effects of transformation thus appeared limited to the biosynthesis of Trp and IAA, with the overall metabolic network in potato being virtually unaffected. These results suggest that transformation with OASA1D may prove effective for the breeding of crops with an increased level of free Trp.

Authors+Show Affiliations

Plant functions and their control, CREST, Japan Science and Technology Agency, 3-4-5 Nihonbashi, Chuo, Tokyo 103-0027, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15912354

Citation

Matsuda, Fumio, et al. "Characterization of Tryptophan-overproducing Potato Transgenic for a Mutant Rice Anthranilate Synthase Alpha-subunit Gene (OASA1D)." Planta, vol. 222, no. 3, 2005, pp. 535-45.
Matsuda F, Yamada T, Miyazawa H, et al. Characterization of tryptophan-overproducing potato transgenic for a mutant rice anthranilate synthase alpha-subunit gene (OASA1D). Planta. 2005;222(3):535-45.
Matsuda, F., Yamada, T., Miyazawa, H., Miyagawa, H., & Wakasa, K. (2005). Characterization of tryptophan-overproducing potato transgenic for a mutant rice anthranilate synthase alpha-subunit gene (OASA1D). Planta, 222(3), 535-45.
Matsuda F, et al. Characterization of Tryptophan-overproducing Potato Transgenic for a Mutant Rice Anthranilate Synthase Alpha-subunit Gene (OASA1D). Planta. 2005;222(3):535-45. PubMed PMID: 15912354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of tryptophan-overproducing potato transgenic for a mutant rice anthranilate synthase alpha-subunit gene (OASA1D). AU - Matsuda,Fumio, AU - Yamada,Tetsuya, AU - Miyazawa,Haruna, AU - Miyagawa,Hisashi, AU - Wakasa,Kyo, Y1 - 2005/05/24/ PY - 2005/01/27/received PY - 2005/04/06/accepted PY - 2005/5/25/pubmed PY - 2006/1/13/medline PY - 2005/5/25/entrez SP - 535 EP - 45 JF - Planta JO - Planta VL - 222 IS - 3 N2 - Potato plants (Solanum tuberosum cv. May Queen) transgenic for OASA1D, which encodes a point mutant of an alpha-subunit of rice (Oryza sativa) anthranilate synthase (AS, EC 4.1.3.27), were generated in order to determine the effects of the mutant gene on levels of free tryptophan (Trp) and AS activity in this important crop. Expression of OASA1D in potato induced a 2- to 20-fold increase in the amount of free Trp. This increase was likely due to a reduction in the sensitivity of AS containing the mutant alpha-subunit to feedback inhibition by Trp. Nontargeted metabolite profiling by high-performance liquid chromatography coupled with ultraviolet photodiode array detection as well as targeted profiling by liquid chromatography coupled with mass spectrometry revealed no marked changes in the levels of other metabolites, with the exception of indole-3-acetic acid (IAA), in the transgenic plants. The level of IAA in the upper part of the shoot was increased by a factor of 8.3-39, depending on the transgenic lines, with no detectable effect on plant growth or development. The effects of transformation thus appeared limited to the biosynthesis of Trp and IAA, with the overall metabolic network in potato being virtually unaffected. These results suggest that transformation with OASA1D may prove effective for the breeding of crops with an increased level of free Trp. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/15912354/Characterization_of_tryptophan_overproducing_potato_transgenic_for_a_mutant_rice_anthranilate_synthase_alpha_subunit_gene__OASA1D__ DB - PRIME DP - Unbound Medicine ER -