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Solid phase micro-extraction coupled with ion mobility spectrometry for the analysis of ephedrine in urine.
J Sep Sci. 2005 May; 28(7):612-8.JS

Abstract

Quantitative solid phase micro-extraction (SPME) coupled with ion mobility spectrometry is demonstrated using the analysis of ephedrine in urine. Since its inception in the 1970's ion mobility spectrometry (IMS) has evolved into a useful technique for laboratories to detect explosives, chemical warfare agents, environment pollutants and, increasingly, for detecting drugs of abuse. Ephedrine is extracted directly from urine samples using SPME and the analyte on the fiber is heated by the IMS desorber unit and vaporized into the drift tube. The analytical procedure was optimized for fiber coating selection, extraction temperature, extraction time, sample pH, and analyte desorption temperature. The carryover effects, ion fragmentation characteristics, peak shapes, and drift times of ephedrine were also evaluated based on the direct interfacing of SPME to IMS. A limit of detection of 50 ng/mL of ephedrine in urine and a linear range of 3 orders of magnitude were obtained, showing that SPME-IMS compares well to other techniques for ephedrine and drug analysis presented in the literature.

Authors+Show Affiliations

Department of Chemistry and Biochemistry, Seton Hall University, 400 South Orange Avenue, South Orange, NJ 07079, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15912729

Citation

Lokhnauth, John K., and Nicholas H. Snow. "Solid Phase Micro-extraction Coupled With Ion Mobility Spectrometry for the Analysis of Ephedrine in Urine." Journal of Separation Science, vol. 28, no. 7, 2005, pp. 612-8.
Lokhnauth JK, Snow NH. Solid phase micro-extraction coupled with ion mobility spectrometry for the analysis of ephedrine in urine. J Sep Sci. 2005;28(7):612-8.
Lokhnauth, J. K., & Snow, N. H. (2005). Solid phase micro-extraction coupled with ion mobility spectrometry for the analysis of ephedrine in urine. Journal of Separation Science, 28(7), 612-8.
Lokhnauth JK, Snow NH. Solid Phase Micro-extraction Coupled With Ion Mobility Spectrometry for the Analysis of Ephedrine in Urine. J Sep Sci. 2005;28(7):612-8. PubMed PMID: 15912729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solid phase micro-extraction coupled with ion mobility spectrometry for the analysis of ephedrine in urine. AU - Lokhnauth,John K, AU - Snow,Nicholas H, PY - 2005/5/26/pubmed PY - 2005/7/6/medline PY - 2005/5/26/entrez SP - 612 EP - 8 JF - Journal of separation science JO - J Sep Sci VL - 28 IS - 7 N2 - Quantitative solid phase micro-extraction (SPME) coupled with ion mobility spectrometry is demonstrated using the analysis of ephedrine in urine. Since its inception in the 1970's ion mobility spectrometry (IMS) has evolved into a useful technique for laboratories to detect explosives, chemical warfare agents, environment pollutants and, increasingly, for detecting drugs of abuse. Ephedrine is extracted directly from urine samples using SPME and the analyte on the fiber is heated by the IMS desorber unit and vaporized into the drift tube. The analytical procedure was optimized for fiber coating selection, extraction temperature, extraction time, sample pH, and analyte desorption temperature. The carryover effects, ion fragmentation characteristics, peak shapes, and drift times of ephedrine were also evaluated based on the direct interfacing of SPME to IMS. A limit of detection of 50 ng/mL of ephedrine in urine and a linear range of 3 orders of magnitude were obtained, showing that SPME-IMS compares well to other techniques for ephedrine and drug analysis presented in the literature. SN - 1615-9306 UR - https://www.unboundmedicine.com/medline/citation/15912729/Solid_phase_micro_extraction_coupled_with_ion_mobility_spectrometry_for_the_analysis_of_ephedrine_in_urine_ L2 - https://doi.org/10.1002/jssc.200401924 DB - PRIME DP - Unbound Medicine ER -