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In vivo bioinsecticidal activity toward Ceratitis capitata (fruit fly) and Callosobruchus maculatus (cowpea weevil) and in vitro bioinsecticidal activity toward different orders of insect pests of a trypsin inhibitor purified from tamarind tree (Tamarindus indica) seeds.
J Agric Food Chem. 2005 Jun 01; 53(11):4381-7.JA

Abstract

A proteinaceous inhibitor with high activity against trypsin-like serine proteinases was purified from seeds of the tamarind tree (Tamarindus indica) by gel filtration on Shephacryl S-200 followed by a reverse-phase HPLC Vidac C18 TP. The inhibitor, called the tamarind trypsin inhibitor (TTI), showed a Mr of 21.42 kDa by mass spectrometry analysis. TTI was a noncompetitive inhibitor with a Ki value of 1.7 x 10(-9) M. In vitro bioinsecticidal activity against insect digestive enzymes from different orders showed that TTI had remarkable activity against enzymes from coleopteran, Anthonomus grandis (29.6%), Zabrotes subfasciatus (51.6%), Callosobruchus maculatus (86.7%), Rhyzopertha dominica(88.2%), and lepidopteron, Plodia interpuncptella (26.7%), Alabama argillacea (53.8%), and Spodoptera frugiperda (75.5%). Also, digestive enzymes from Diptera, Ceratitis capitata (fruit fly), were inhibited (52.9%). In vivo bioinsecticidal assays toward C. capitata and C. maculatus larvae were developed. The concentration of TTI (w/w) in the artificial seed necessary to cause 50% mortality (LD50) of larvae was 3.6%, and that to reduce mass larvae by 50.0% (ED50) was 3.2%. Furthermore, the mass C. capitata larvae were affected at 53.2% and produced approximately 34% mortality at a level of 4.0% (w/w) of TTI incorporated in artificial diets.

Authors+Show Affiliations

Araújo CL
Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
Bezerra IW
No affiliation info available
Oliveira AS
No affiliation info available
Moura FT
No affiliation info available
Macedo LL
No affiliation info available
Gomes CE
No affiliation info available
Barbosa AE
No affiliation info available
Macedo FP
No affiliation info available
Souza TM
No affiliation info available
Franco OL
No affiliation info available
Bloch-J C
No affiliation info available
Sales MP
No affiliation info available

MeSH

Ceratitis capitataElectrophoresis, Polyacrylamide GelInsecticidesSeedsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTamarindusTrypsin InhibitorsWeevils

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15913299

Citation

Araújo, Carina L., et al. "In Vivo Bioinsecticidal Activity Toward Ceratitis Capitata (fruit Fly) and Callosobruchus Maculatus (cowpea Weevil) and in Vitro Bioinsecticidal Activity Toward Different Orders of Insect Pests of a Trypsin Inhibitor Purified From Tamarind Tree (Tamarindus Indica) Seeds." Journal of Agricultural and Food Chemistry, vol. 53, no. 11, 2005, pp. 4381-7.
Araújo CL, Bezerra IW, Oliveira AS, et al. In vivo bioinsecticidal activity toward Ceratitis capitata (fruit fly) and Callosobruchus maculatus (cowpea weevil) and in vitro bioinsecticidal activity toward different orders of insect pests of a trypsin inhibitor purified from tamarind tree (Tamarindus indica) seeds. J Agric Food Chem. 2005;53(11):4381-7.
Araújo, C. L., Bezerra, I. W., Oliveira, A. S., Moura, F. T., Macedo, L. L., Gomes, C. E., Barbosa, A. E., Macedo, F. P., Souza, T. M., Franco, O. L., Bloch-J, C., & Sales, M. P. (2005). In vivo bioinsecticidal activity toward Ceratitis capitata (fruit fly) and Callosobruchus maculatus (cowpea weevil) and in vitro bioinsecticidal activity toward different orders of insect pests of a trypsin inhibitor purified from tamarind tree (Tamarindus indica) seeds. Journal of Agricultural and Food Chemistry, 53(11), 4381-7.
Araújo CL, et al. In Vivo Bioinsecticidal Activity Toward Ceratitis Capitata (fruit Fly) and Callosobruchus Maculatus (cowpea Weevil) and in Vitro Bioinsecticidal Activity Toward Different Orders of Insect Pests of a Trypsin Inhibitor Purified From Tamarind Tree (Tamarindus Indica) Seeds. J Agric Food Chem. 2005 Jun 1;53(11):4381-7. PubMed PMID: 15913299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo bioinsecticidal activity toward Ceratitis capitata (fruit fly) and Callosobruchus maculatus (cowpea weevil) and in vitro bioinsecticidal activity toward different orders of insect pests of a trypsin inhibitor purified from tamarind tree (Tamarindus indica) seeds. AU - Araújo,Carina L, AU - Bezerra,Ingrid W L, AU - Oliveira,Adeliana S, AU - Moura,Fabiano T, AU - Macedo,Leonardo L P, AU - Gomes,Carlos E M, AU - Barbosa,Aulus E A D, AU - Macedo,Francisco P, AU - Souza,Tánia M S, AU - Franco,Octavio L, AU - Bloch-J,Carlos, AU - Sales,Mauricio P, PY - 2005/5/26/pubmed PY - 2005/7/6/medline PY - 2005/5/26/entrez SP - 4381 EP - 7 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 53 IS - 11 N2 - A proteinaceous inhibitor with high activity against trypsin-like serine proteinases was purified from seeds of the tamarind tree (Tamarindus indica) by gel filtration on Shephacryl S-200 followed by a reverse-phase HPLC Vidac C18 TP. The inhibitor, called the tamarind trypsin inhibitor (TTI), showed a Mr of 21.42 kDa by mass spectrometry analysis. TTI was a noncompetitive inhibitor with a Ki value of 1.7 x 10(-9) M. In vitro bioinsecticidal activity against insect digestive enzymes from different orders showed that TTI had remarkable activity against enzymes from coleopteran, Anthonomus grandis (29.6%), Zabrotes subfasciatus (51.6%), Callosobruchus maculatus (86.7%), Rhyzopertha dominica(88.2%), and lepidopteron, Plodia interpuncptella (26.7%), Alabama argillacea (53.8%), and Spodoptera frugiperda (75.5%). Also, digestive enzymes from Diptera, Ceratitis capitata (fruit fly), were inhibited (52.9%). In vivo bioinsecticidal assays toward C. capitata and C. maculatus larvae were developed. The concentration of TTI (w/w) in the artificial seed necessary to cause 50% mortality (LD50) of larvae was 3.6%, and that to reduce mass larvae by 50.0% (ED50) was 3.2%. Furthermore, the mass C. capitata larvae were affected at 53.2% and produced approximately 34% mortality at a level of 4.0% (w/w) of TTI incorporated in artificial diets. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15913299/In_vivo_bioinsecticidal_activity_toward_Ceratitis_capitata__fruit_fly__and_Callosobruchus_maculatus__cowpea_weevil__and_in_vitro_bioinsecticidal_activity_toward_different_orders_of_insect_pests_of_a_trypsin_inhibitor_purified_from_tamarind_tree__Tamarindus_indica__seeds_ DB - PRIME DP - Unbound Medicine ER -
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