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In vitro and in vivo effects of isolated fractions of Brazilian propolis on caries development.
J Ethnopharmacol. 2005 Oct 03; 101(1-3):110-5.JE

Abstract

Recently, two chemically different types of Brazilian propolis (type-3 and -12) were shown to have cariostatic properties. This study aimed to evaluate the influence of their isolated fractions on mutans streptococci viability, glucosyltransferases (GTFs) activity and caries development in rats. The ethanolic extracts of propolis (EEPs) were serially fractionated into hexane (H-fr), chloroform, ethyl acetate, and ethanol. The ability of the four fractions and EEP to inhibit Streptococcus mutans and Streptococcus sobrinus growth and adherence to a glass surface was examined. The effect on GTFs B and C activity was also determined. For the caries study, 60 Wistar rats infected with Streptococcus sobrinus were treated topically twice daily as follows: (1) EEP type-3, (2) H-fr type-3, (3) EEP type-12, (4) H-fr type-12, and (5) control. In general, the H-fr from both types of propolis showed the highest antibacterial activity and GTFs inhibition. Furthermore, the EEP and H-fr type-3 and -12 were equally effective in reducing dental caries in rats. The data suggest that the putative cariostatic compounds of propolis type-3 and -12 are mostly non-polar; and H-fr should be the fraction of choice for identifying further potentially novel anti-caries agents.

Authors+Show Affiliations

Department of Physiological Sciences, Dentistry Faculty of Piracicaba, State University of Campinas, Av. Limeira, 901 PIRACICABA-SP, CEP: 13414-903, Brazil. mhayacibara@wnet.com.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15913934

Citation

Hayacibara, Mitsue F., et al. "In Vitro and in Vivo Effects of Isolated Fractions of Brazilian Propolis On Caries Development." Journal of Ethnopharmacology, vol. 101, no. 1-3, 2005, pp. 110-5.
Hayacibara MF, Koo H, Rosalen PL, et al. In vitro and in vivo effects of isolated fractions of Brazilian propolis on caries development. J Ethnopharmacol. 2005;101(1-3):110-5.
Hayacibara, M. F., Koo, H., Rosalen, P. L., Duarte, S., Franco, E. M., Bowen, W. H., Ikegaki, M., & Cury, J. A. (2005). In vitro and in vivo effects of isolated fractions of Brazilian propolis on caries development. Journal of Ethnopharmacology, 101(1-3), 110-5.
Hayacibara MF, et al. In Vitro and in Vivo Effects of Isolated Fractions of Brazilian Propolis On Caries Development. J Ethnopharmacol. 2005 Oct 3;101(1-3):110-5. PubMed PMID: 15913934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro and in vivo effects of isolated fractions of Brazilian propolis on caries development. AU - Hayacibara,Mitsue F, AU - Koo,Hyun, AU - Rosalen,Pedro L, AU - Duarte,Simone, AU - Franco,Eliane M, AU - Bowen,William H, AU - Ikegaki,Masaharu, AU - Cury,Jaime A, PY - 2004/08/12/received PY - 2005/03/18/revised PY - 2005/04/07/accepted PY - 2005/5/26/pubmed PY - 2005/12/29/medline PY - 2005/5/26/entrez SP - 110 EP - 5 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 101 IS - 1-3 N2 - Recently, two chemically different types of Brazilian propolis (type-3 and -12) were shown to have cariostatic properties. This study aimed to evaluate the influence of their isolated fractions on mutans streptococci viability, glucosyltransferases (GTFs) activity and caries development in rats. The ethanolic extracts of propolis (EEPs) were serially fractionated into hexane (H-fr), chloroform, ethyl acetate, and ethanol. The ability of the four fractions and EEP to inhibit Streptococcus mutans and Streptococcus sobrinus growth and adherence to a glass surface was examined. The effect on GTFs B and C activity was also determined. For the caries study, 60 Wistar rats infected with Streptococcus sobrinus were treated topically twice daily as follows: (1) EEP type-3, (2) H-fr type-3, (3) EEP type-12, (4) H-fr type-12, and (5) control. In general, the H-fr from both types of propolis showed the highest antibacterial activity and GTFs inhibition. Furthermore, the EEP and H-fr type-3 and -12 were equally effective in reducing dental caries in rats. The data suggest that the putative cariostatic compounds of propolis type-3 and -12 are mostly non-polar; and H-fr should be the fraction of choice for identifying further potentially novel anti-caries agents. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/15913934/In_vitro_and_in_vivo_effects_of_isolated_fractions_of_Brazilian_propolis_on_caries_development_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(05)00246-1 DB - PRIME DP - Unbound Medicine ER -