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Pattern of enterotoxin genes seg, seh, sei and sej positive Staphylococcus aureus isolated from bovine mastitis.
Vet Microbiol. 2005 Jul 01; 108(3-4):243-9.VM

Abstract

PCR detection of the genes encoding the newly described staphylococcal enterotoxins (SE) SEG, SEH, SEI and SEJ was carried out for 104 randomly selected Staphylococcus aureus field strains isolated from cases of bovine mastitis. Sixty-one (58.7%) isolates were positive for one or more of these novel enterotoxin genes. Thirty-six field strains were classified as carrier of seg, 22 of sei gene and 23 were positive for sej gene. None of the 104 investigated ruminant S. aureus strains carried the seh gene. Thirty-seven of these S. aureus strains showed a combination of genes encoding enterotoxin types SEA to SEE or toxic shock syndrome toxin 1 (TSST 1). Thirteen cultures harboured only one, 28 two, 12 three and 8 four enterotoxin genes. Among the 61 S. aureus field strains 14 (23.0%) were positive for the genes encoding SEJ and SED and 10 (16.4%) isolates for those encoding SEG and SEI. Isolates harbouring the sed/sej genes were further characterized by macrorestriction analysis and pulsed-field-gelelectrophoresis (Pfge). Macrorestriction analysis revealed six patterns. Nine of these14 S. aureus isolates (64.3%) exhibited two patterns with a high degree of relationship (>80%).

Authors+Show Affiliations

Hessisches Landeslabor, Abteilung II (Veterinärmedizin), Marburger Str. 54, 35396 Giessen, Germany. m.zschoeck@lhl.hessen.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15916869

Citation

Zschöck, M, et al. "Pattern of Enterotoxin Genes Seg, Seh, Sei and Sej Positive Staphylococcus Aureus Isolated From Bovine Mastitis." Veterinary Microbiology, vol. 108, no. 3-4, 2005, pp. 243-9.
Zschöck M, Kloppert B, Wolter W, et al. Pattern of enterotoxin genes seg, seh, sei and sej positive Staphylococcus aureus isolated from bovine mastitis. Vet Microbiol. 2005;108(3-4):243-9.
Zschöck, M., Kloppert, B., Wolter, W., Hamann, H. P., & Lämmler, C. h. (2005). Pattern of enterotoxin genes seg, seh, sei and sej positive Staphylococcus aureus isolated from bovine mastitis. Veterinary Microbiology, 108(3-4), 243-9.
Zschöck M, et al. Pattern of Enterotoxin Genes Seg, Seh, Sei and Sej Positive Staphylococcus Aureus Isolated From Bovine Mastitis. Vet Microbiol. 2005 Jul 1;108(3-4):243-9. PubMed PMID: 15916869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pattern of enterotoxin genes seg, seh, sei and sej positive Staphylococcus aureus isolated from bovine mastitis. AU - Zschöck,M, AU - Kloppert,Bärbel, AU - Wolter,W, AU - Hamann,H P, AU - Lämmler,Ch, PY - 2004/09/30/received PY - 2005/02/21/revised PY - 2005/02/22/accepted PY - 2005/5/27/pubmed PY - 2005/8/18/medline PY - 2005/5/27/entrez SP - 243 EP - 9 JF - Veterinary microbiology JO - Vet Microbiol VL - 108 IS - 3-4 N2 - PCR detection of the genes encoding the newly described staphylococcal enterotoxins (SE) SEG, SEH, SEI and SEJ was carried out for 104 randomly selected Staphylococcus aureus field strains isolated from cases of bovine mastitis. Sixty-one (58.7%) isolates were positive for one or more of these novel enterotoxin genes. Thirty-six field strains were classified as carrier of seg, 22 of sei gene and 23 were positive for sej gene. None of the 104 investigated ruminant S. aureus strains carried the seh gene. Thirty-seven of these S. aureus strains showed a combination of genes encoding enterotoxin types SEA to SEE or toxic shock syndrome toxin 1 (TSST 1). Thirteen cultures harboured only one, 28 two, 12 three and 8 four enterotoxin genes. Among the 61 S. aureus field strains 14 (23.0%) were positive for the genes encoding SEJ and SED and 10 (16.4%) isolates for those encoding SEG and SEI. Isolates harbouring the sed/sej genes were further characterized by macrorestriction analysis and pulsed-field-gelelectrophoresis (Pfge). Macrorestriction analysis revealed six patterns. Nine of these14 S. aureus isolates (64.3%) exhibited two patterns with a high degree of relationship (>80%). SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/15916869/Pattern_of_enterotoxin_genes_seg_seh_sei_and_sej_positive_Staphylococcus_aureus_isolated_from_bovine_mastitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(05)00109-4 DB - PRIME DP - Unbound Medicine ER -