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In vitro and in vivo immunosuppressive activity of Spica Prunellae ethanol extract on the immune responses in mice.
J Ethnopharmacol. 2005 Oct 03; 101(1-3):31-6.JE

Abstract

The immunosuppressive activity of the ethanol extract of Spica Prunellae (Prunella vulgaris fruiting spikes) (EESP) consisting of a mixture of triterpenoids, flavonoids, tannins and polysaccharide was studied on the immune responses in mice. The effects of EESP on mice splenocyte proliferation in vitro were measured. EESP significantly suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. Furthermore, the effects of EESP at three dose levels (a single dose of 0.25, 0.5, and 1.0 mg) on the humoral and cellular immune responses of mice subcutaneously immunized with ovalbumin (OVA) were examined. ICR mice were immunized subcutaneously with OVA on day 0 and 14. Starting on the day of immunization, the mice were administered subcutaneously with EESP at 0.25, 0.5, and 1.0 mg at intervals of 7 days for a total of five doses. On day 28, antigen-specific antibodies in serum, mitogen- and OVA-induced splenocyte proliferation were investigated. EESP significantly suppressed Con A-, LPS- and OVA-induced splenocyte proliferation in the immunized mice in a dose-dependent manner. Total IgG, IgG1 and IgG2b levels in the immunized mice were significantly reduced by EESP. Moreover, the suppressing effects on the antibody responses to OVA in the immunized mice were dose-dependently enhanced according to the increase of EESP. The results suggest that EESP could suppress the cellular and humoral response in mice.

Authors+Show Affiliations

College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. sunhx@zju.edu.cnNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15919165

Citation

Sun, Hong-Xiang, et al. "In Vitro and in Vivo Immunosuppressive Activity of Spica Prunellae Ethanol Extract On the Immune Responses in Mice." Journal of Ethnopharmacology, vol. 101, no. 1-3, 2005, pp. 31-6.
Sun HX, Qin F, Pan YJ. In vitro and in vivo immunosuppressive activity of Spica Prunellae ethanol extract on the immune responses in mice. J Ethnopharmacol. 2005;101(1-3):31-6.
Sun, H. X., Qin, F., & Pan, Y. J. (2005). In vitro and in vivo immunosuppressive activity of Spica Prunellae ethanol extract on the immune responses in mice. Journal of Ethnopharmacology, 101(1-3), 31-6.
Sun HX, Qin F, Pan YJ. In Vitro and in Vivo Immunosuppressive Activity of Spica Prunellae Ethanol Extract On the Immune Responses in Mice. J Ethnopharmacol. 2005 Oct 3;101(1-3):31-6. PubMed PMID: 15919165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro and in vivo immunosuppressive activity of Spica Prunellae ethanol extract on the immune responses in mice. AU - Sun,Hong-Xiang, AU - Qin,Feng, AU - Pan,Yuan-Jiang, PY - 2004/04/28/received PY - 2005/02/28/revised PY - 2005/03/20/accepted PY - 2005/5/28/pubmed PY - 2005/12/29/medline PY - 2005/5/28/entrez SP - 31 EP - 6 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 101 IS - 1-3 N2 - The immunosuppressive activity of the ethanol extract of Spica Prunellae (Prunella vulgaris fruiting spikes) (EESP) consisting of a mixture of triterpenoids, flavonoids, tannins and polysaccharide was studied on the immune responses in mice. The effects of EESP on mice splenocyte proliferation in vitro were measured. EESP significantly suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. Furthermore, the effects of EESP at three dose levels (a single dose of 0.25, 0.5, and 1.0 mg) on the humoral and cellular immune responses of mice subcutaneously immunized with ovalbumin (OVA) were examined. ICR mice were immunized subcutaneously with OVA on day 0 and 14. Starting on the day of immunization, the mice were administered subcutaneously with EESP at 0.25, 0.5, and 1.0 mg at intervals of 7 days for a total of five doses. On day 28, antigen-specific antibodies in serum, mitogen- and OVA-induced splenocyte proliferation were investigated. EESP significantly suppressed Con A-, LPS- and OVA-induced splenocyte proliferation in the immunized mice in a dose-dependent manner. Total IgG, IgG1 and IgG2b levels in the immunized mice were significantly reduced by EESP. Moreover, the suppressing effects on the antibody responses to OVA in the immunized mice were dose-dependently enhanced according to the increase of EESP. The results suggest that EESP could suppress the cellular and humoral response in mice. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/15919165/In_vitro_and_in_vivo_immunosuppressive_activity_of_Spica_Prunellae_ethanol_extract_on_the_immune_responses_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(05)00262-X DB - PRIME DP - Unbound Medicine ER -