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Reactivity of Langerhans cells in human reconstructed epidermis to known allergens and UV radiation.
Toxicol In Vitro. 2005 Sep; 19(6):787-95.TV

Abstract

Epidermal Langerhans cells are the outmost guards of our immune defence system. These cells are directly involved in phenomena such as contact hypersensitivity and UV-induced immunosuppression. Some years ago we succeeded in introducing CD34(+)-derived Langerhans cells into a reconstructed human epidermis. Here we describe their reactivity after topical exposure of the reconstructed epidermis to known allergens, allergen-inducible cytokines, irritants and UV irradiation. Exposure to allergens for 24 h resulted in an activated appearance of the Langerhans cells and in some cases a decrease in their number. Concomitantly, IL-1beta and CD86 mRNA over-expressions were detected in the reconstructed epidermis. A topical treatment with TNF-alpha or IL-1beta revealed that both cytokines induced an activated appearance of the Langerhans cells as early as 4 h following application. Irritants had no effect on the integrated Langerhans cells. Exposure of the reconstructed epidermis to Solar Simulated Radiation caused a dramatic decrease in the number of Langerhans cells and a loss of dendricity in the remaining cells 24 h after irradiation. The topical application of a large spectrum UVA/B filter before irradiation prevented these UV-induced alterations. In our hands, this model provides a promising tool to evaluate the sensitization potential of new compounds and to validate the efficacy of sunscreens to prevent UV-induced immunosuppression.

Authors+Show Affiliations

L'OREAL Recherche, Centre Charles Zviak, 90 rue du Général Roguet, 92583 Clichy Cedex, France. vfacy@rd.loreal.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15921883

Citation

Facy, V, et al. "Reactivity of Langerhans Cells in Human Reconstructed Epidermis to Known Allergens and UV Radiation." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 19, no. 6, 2005, pp. 787-95.
Facy V, Flouret V, Régnier M, et al. Reactivity of Langerhans cells in human reconstructed epidermis to known allergens and UV radiation. Toxicol In Vitro. 2005;19(6):787-95.
Facy, V., Flouret, V., Régnier, M., & Schmidt, R. (2005). Reactivity of Langerhans cells in human reconstructed epidermis to known allergens and UV radiation. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 19(6), 787-95.
Facy V, et al. Reactivity of Langerhans Cells in Human Reconstructed Epidermis to Known Allergens and UV Radiation. Toxicol In Vitro. 2005;19(6):787-95. PubMed PMID: 15921883.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reactivity of Langerhans cells in human reconstructed epidermis to known allergens and UV radiation. AU - Facy,V, AU - Flouret,V, AU - Régnier,M, AU - Schmidt,R, PY - 2004/10/11/received PY - 2005/02/25/revised PY - 2005/03/17/accepted PY - 2005/6/1/pubmed PY - 2005/9/28/medline PY - 2005/6/1/entrez SP - 787 EP - 95 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 19 IS - 6 N2 - Epidermal Langerhans cells are the outmost guards of our immune defence system. These cells are directly involved in phenomena such as contact hypersensitivity and UV-induced immunosuppression. Some years ago we succeeded in introducing CD34(+)-derived Langerhans cells into a reconstructed human epidermis. Here we describe their reactivity after topical exposure of the reconstructed epidermis to known allergens, allergen-inducible cytokines, irritants and UV irradiation. Exposure to allergens for 24 h resulted in an activated appearance of the Langerhans cells and in some cases a decrease in their number. Concomitantly, IL-1beta and CD86 mRNA over-expressions were detected in the reconstructed epidermis. A topical treatment with TNF-alpha or IL-1beta revealed that both cytokines induced an activated appearance of the Langerhans cells as early as 4 h following application. Irritants had no effect on the integrated Langerhans cells. Exposure of the reconstructed epidermis to Solar Simulated Radiation caused a dramatic decrease in the number of Langerhans cells and a loss of dendricity in the remaining cells 24 h after irradiation. The topical application of a large spectrum UVA/B filter before irradiation prevented these UV-induced alterations. In our hands, this model provides a promising tool to evaluate the sensitization potential of new compounds and to validate the efficacy of sunscreens to prevent UV-induced immunosuppression. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/15921883/Reactivity_of_Langerhans_cells_in_human_reconstructed_epidermis_to_known_allergens_and_UV_radiation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(05)00058-5 DB - PRIME DP - Unbound Medicine ER -