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Fatty acid metabolism in Atlantic salmon (Salmo salar L.) hepatocytes and influence of dietary vegetable oil.
Biochim Biophys Acta 2005; 1734(3):277-88BB

Abstract

Isolated hepatocytes from Atlantic salmon (Salmo salar), fed diets containing either 100% fish oil or a vegetable oil blend replacing 75% of the fish oil, were incubated with a range of seven (14)C-labelled fatty acids. The fatty acids were [1-(14)C]16:0, [1-(14)C]18:1n-9, 91-(14)C]18:2n-6, [1-(14)C]18:3n-3, [1-(14)C]20:4n-6, [1-(14)C]20:5n-3, and [1-(14)C]22:6n-3. After 2 h of incubation, the hepatocytes and medium were analysed for acid soluble products, incorporation into lipid classes, and hepatocytes for desaturation and elongation. Uptake into hepatocytes was highest with [1-(14)C]18:2n-6 and [1-(14)C]20:5n-3 and lowest with [1-(14)C]16:0. The highest recovery of radioactivity in the cells was found in triacylglycerols. Of the phospholipids, the highest recovery was found in phosphatidylcholine, with [1-(14)C]16:0 and [1-(14)C]22:6n-3 being the most prominent fatty acids. The rates of beta-oxidation were as follows: 20:4n-6>18:2n-6=16:0>18:1n-9>22:6n-3=18:3n-3=20:5n-3. Of the fatty acids taken up by the hepatocytes, [1-(14)C]16:0 and [1-(14)C]18:1n-9 were subsequently exported the most, with the majority of radioactivity recovered in phospholipids and triacylglycerols, respectively. The major products from desaturation and elongation were generally one cycle of elongation of the fatty acids. Diet had a clear effect on the overall lipid metabolism, with replacing 75% of the fish oil with vegetable oil resulting in decreased uptake of all fatty acids and reduced incorporation of fatty acids into cellular lipids, but increased beta-oxidation activity and higher recovery in products of desaturation and elongation of [1-(14)C]18:2n-6 and [1-(14)C]18:3n-3.

Authors+Show Affiliations

National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029, Nordnes, 5817 Bergen, Norway. ingunn.stubhaug@nifes.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15921956

Citation

Stubhaug, Ingunn, et al. "Fatty Acid Metabolism in Atlantic Salmon (Salmo Salar L.) Hepatocytes and Influence of Dietary Vegetable Oil." Biochimica Et Biophysica Acta, vol. 1734, no. 3, 2005, pp. 277-88.
Stubhaug I, Tocher DR, Bell JG, et al. Fatty acid metabolism in Atlantic salmon (Salmo salar L.) hepatocytes and influence of dietary vegetable oil. Biochim Biophys Acta. 2005;1734(3):277-88.
Stubhaug, I., Tocher, D. R., Bell, J. G., Dick, J. R., & Torstensen, B. E. (2005). Fatty acid metabolism in Atlantic salmon (Salmo salar L.) hepatocytes and influence of dietary vegetable oil. Biochimica Et Biophysica Acta, 1734(3), pp. 277-88.
Stubhaug I, et al. Fatty Acid Metabolism in Atlantic Salmon (Salmo Salar L.) Hepatocytes and Influence of Dietary Vegetable Oil. Biochim Biophys Acta. 2005 Jun 1;1734(3):277-88. PubMed PMID: 15921956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fatty acid metabolism in Atlantic salmon (Salmo salar L.) hepatocytes and influence of dietary vegetable oil. AU - Stubhaug,Ingunn, AU - Tocher,Douglas R, AU - Bell,J Gordon, AU - Dick,James R, AU - Torstensen,Bente E, PY - 2005/03/16/received PY - 2005/04/20/revised PY - 2005/04/20/accepted PY - 2005/6/1/pubmed PY - 2005/7/15/medline PY - 2005/6/1/entrez SP - 277 EP - 88 JF - Biochimica et biophysica acta JO - Biochim. Biophys. Acta VL - 1734 IS - 3 N2 - Isolated hepatocytes from Atlantic salmon (Salmo salar), fed diets containing either 100% fish oil or a vegetable oil blend replacing 75% of the fish oil, were incubated with a range of seven (14)C-labelled fatty acids. The fatty acids were [1-(14)C]16:0, [1-(14)C]18:1n-9, 91-(14)C]18:2n-6, [1-(14)C]18:3n-3, [1-(14)C]20:4n-6, [1-(14)C]20:5n-3, and [1-(14)C]22:6n-3. After 2 h of incubation, the hepatocytes and medium were analysed for acid soluble products, incorporation into lipid classes, and hepatocytes for desaturation and elongation. Uptake into hepatocytes was highest with [1-(14)C]18:2n-6 and [1-(14)C]20:5n-3 and lowest with [1-(14)C]16:0. The highest recovery of radioactivity in the cells was found in triacylglycerols. Of the phospholipids, the highest recovery was found in phosphatidylcholine, with [1-(14)C]16:0 and [1-(14)C]22:6n-3 being the most prominent fatty acids. The rates of beta-oxidation were as follows: 20:4n-6>18:2n-6=16:0>18:1n-9>22:6n-3=18:3n-3=20:5n-3. Of the fatty acids taken up by the hepatocytes, [1-(14)C]16:0 and [1-(14)C]18:1n-9 were subsequently exported the most, with the majority of radioactivity recovered in phospholipids and triacylglycerols, respectively. The major products from desaturation and elongation were generally one cycle of elongation of the fatty acids. Diet had a clear effect on the overall lipid metabolism, with replacing 75% of the fish oil with vegetable oil resulting in decreased uptake of all fatty acids and reduced incorporation of fatty acids into cellular lipids, but increased beta-oxidation activity and higher recovery in products of desaturation and elongation of [1-(14)C]18:2n-6 and [1-(14)C]18:3n-3. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/15921956/Fatty_acid_metabolism_in_Atlantic_salmon__Salmo_salar_L___hepatocytes_and_influence_of_dietary_vegetable_oil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1388-1981(05)00088-0 DB - PRIME DP - Unbound Medicine ER -