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IL-1 alpha stimulates the formation of osteoclast-like cells by increasing M-CSF and PGE2 production and decreasing OPG production by osteoblasts.
Life Sci. 2005 Jun 24; 77(6):615-26.LS

Abstract

Interleukin-1alpha (IL-1alpha) is one of the most potent bone-resorbing factors involved in the bone loss that is associated with inflammation. We examined the effect of the inflammatory mediator IL-1alpha on the expression of macrophage colony-stimulating factor (M-CSF), osteoprotegerin (OPG), and prostaglandin E2 (PGE2) in rat osteoblasts, and the indirect effect of IL-1alpha on the formation of osteoclast-like cells. Osteoblasts were cultured in alpha-minimum essential medium containing 10% fetal bovine serum with or without 100 units/ml of IL-1alpha for up to 14 days. The gene and protein expression of M-CSF and OPG were estimated by determining mRNA levels using the real-time polymerase chain reaction and protein levels using Western blot analysis. PGE2 expression was determined using an enzyme-linked immunosorbent assay. The formation of osteoclast-like cells was estimated using tartrate-resistant acid phosphatase (TRAP) staining of osteoclast precursors in culture with conditioned medium from IL-1alpha-treated osteoblasts and the soluble receptor activator of NF-kappaB ligand (RANKL). M-CSF and PGE2 expression in osteoblasts increased markedly in cells cultured with IL-1alpha, whereas OPG expression decreased. The conditioned medium containing M-CSF and PGE2 produced by IL-1alpha-treated osteoblasts and soluble RANKL increased the TRAP staining of osteoclast precursors. These results suggest that IL-1alpha stimulated the formation of osteoclast-like cells via an increase in M-CSF and PGE2 production, and a decrease in OPG production by osteoblasts.

Authors+Show Affiliations

Department of Endodontics, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15921993

Citation

Tanabe, Natsuko, et al. "IL-1 Alpha Stimulates the Formation of Osteoclast-like Cells By Increasing M-CSF and PGE2 Production and Decreasing OPG Production By Osteoblasts." Life Sciences, vol. 77, no. 6, 2005, pp. 615-26.
Tanabe N, Maeno M, Suzuki N, et al. IL-1 alpha stimulates the formation of osteoclast-like cells by increasing M-CSF and PGE2 production and decreasing OPG production by osteoblasts. Life Sci. 2005;77(6):615-26.
Tanabe, N., Maeno, M., Suzuki, N., Fujisaki, K., Tanaka, H., Ogiso, B., & Ito, K. (2005). IL-1 alpha stimulates the formation of osteoclast-like cells by increasing M-CSF and PGE2 production and decreasing OPG production by osteoblasts. Life Sciences, 77(6), 615-26.
Tanabe N, et al. IL-1 Alpha Stimulates the Formation of Osteoclast-like Cells By Increasing M-CSF and PGE2 Production and Decreasing OPG Production By Osteoblasts. Life Sci. 2005 Jun 24;77(6):615-26. PubMed PMID: 15921993.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-1 alpha stimulates the formation of osteoclast-like cells by increasing M-CSF and PGE2 production and decreasing OPG production by osteoblasts. AU - Tanabe,Natsuko, AU - Maeno,Masao, AU - Suzuki,Naoto, AU - Fujisaki,Kyosuke, AU - Tanaka,Hideki, AU - Ogiso,Bunnai, AU - Ito,Koichi, Y1 - 2005/02/25/ PY - 2004/07/19/received PY - 2004/10/18/accepted PY - 2005/6/1/pubmed PY - 2005/7/6/medline PY - 2005/6/1/entrez SP - 615 EP - 26 JF - Life sciences JO - Life Sci VL - 77 IS - 6 N2 - Interleukin-1alpha (IL-1alpha) is one of the most potent bone-resorbing factors involved in the bone loss that is associated with inflammation. We examined the effect of the inflammatory mediator IL-1alpha on the expression of macrophage colony-stimulating factor (M-CSF), osteoprotegerin (OPG), and prostaglandin E2 (PGE2) in rat osteoblasts, and the indirect effect of IL-1alpha on the formation of osteoclast-like cells. Osteoblasts were cultured in alpha-minimum essential medium containing 10% fetal bovine serum with or without 100 units/ml of IL-1alpha for up to 14 days. The gene and protein expression of M-CSF and OPG were estimated by determining mRNA levels using the real-time polymerase chain reaction and protein levels using Western blot analysis. PGE2 expression was determined using an enzyme-linked immunosorbent assay. The formation of osteoclast-like cells was estimated using tartrate-resistant acid phosphatase (TRAP) staining of osteoclast precursors in culture with conditioned medium from IL-1alpha-treated osteoblasts and the soluble receptor activator of NF-kappaB ligand (RANKL). M-CSF and PGE2 expression in osteoblasts increased markedly in cells cultured with IL-1alpha, whereas OPG expression decreased. The conditioned medium containing M-CSF and PGE2 produced by IL-1alpha-treated osteoblasts and soluble RANKL increased the TRAP staining of osteoclast precursors. These results suggest that IL-1alpha stimulated the formation of osteoclast-like cells via an increase in M-CSF and PGE2 production, and a decrease in OPG production by osteoblasts. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/15921993/IL_1_alpha_stimulates_the_formation_of_osteoclast_like_cells_by_increasing_M_CSF_and_PGE2_production_and_decreasing_OPG_production_by_osteoblasts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(05)00198-0 DB - PRIME DP - Unbound Medicine ER -