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Nociceptin/orphanin FQ stimulates human monocyte chemotaxis via NOP receptor activation.
Peptides. 2005 Aug; 26(8):1497-502.P

Abstract

Nociceptin/orphanin FQ (N/OFQ) produces several biological actions by activating the N/OFQ peptide receptor (NOP). It has been previously shown that N/OFQ stimulates leukocyte chemotaxis both in vitro and in vivo. In the present study we investigated the ability of N/OFQ, in comparison with the proinflammatory peptide formyl-Met-Leu-Phe (fMLP), to stimulate human neutrophil and monocyte chemotaxis and the release of lysozyme and superoxide anion (O2-) production from neutrophils. fMLP stimulated all the leukocyte functions examined. N/OFQ stimulated monocyte (pEC50 12.15) but not neutrophil chemotaxis. The production of O2- from neutrophils was not affected by N/OFQ while the release of lysozyme was increased in a concentration dependent manner (pEC50 11.00) although the maximal effects evoked by N/OFQ were about half of those of fMLP. The NOP ligands [Arg14, Lys15]N/OFQ, N/OFQ(1-13)NH2, Ro 64-6198, UFP-101 and the opioid antagonist naloxone were used for pharmacologically characterizing the receptor involved in the monocyte chemoattractant action of N/OFQ. [Arg14, Lys15]N/OFQ, N/OFQ(1-13)NH2, and Ro 64-6198 mimicked the action of N/OFQ showing similar maximal effects and the following order of potency: [Arg14, Lys15]N/OFQ (pEC50 13.22)>Ro 64-6198 (pEC50 12.96)>N/OFQ(1-13)NH2 (pEC50 12.67)>N/OFQ (pEC50 12.15). Moreover, the monocyte chemoattractant action of N/OFQ was not modified by naloxone 1 microM while antagonized by UFP-101 10 microM (pA2 7.00). Thus, the order of potency of agonists and the antagonist selectivity demonstrated that N/OFQ stimulates human monocyte chemotaxis via NOP receptor activation.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, University of Ferrara, via Fossato di Mortara, 19, 44100 Ferrara, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15922491

Citation

Trombella, S, et al. "Nociceptin/orphanin FQ Stimulates Human Monocyte Chemotaxis Via NOP Receptor Activation." Peptides, vol. 26, no. 8, 2005, pp. 1497-502.
Trombella S, Vergura R, Falzarano S, et al. Nociceptin/orphanin FQ stimulates human monocyte chemotaxis via NOP receptor activation. Peptides. 2005;26(8):1497-502.
Trombella, S., Vergura, R., Falzarano, S., Guerrini, R., Calo, G., & Spisani, S. (2005). Nociceptin/orphanin FQ stimulates human monocyte chemotaxis via NOP receptor activation. Peptides, 26(8), 1497-502.
Trombella S, et al. Nociceptin/orphanin FQ Stimulates Human Monocyte Chemotaxis Via NOP Receptor Activation. Peptides. 2005;26(8):1497-502. PubMed PMID: 15922491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nociceptin/orphanin FQ stimulates human monocyte chemotaxis via NOP receptor activation. AU - Trombella,S, AU - Vergura,R, AU - Falzarano,S, AU - Guerrini,R, AU - Calo,G, AU - Spisani,S, PY - 2005/6/1/pubmed PY - 2005/12/22/medline PY - 2005/6/1/entrez SP - 1497 EP - 502 JF - Peptides JO - Peptides VL - 26 IS - 8 N2 - Nociceptin/orphanin FQ (N/OFQ) produces several biological actions by activating the N/OFQ peptide receptor (NOP). It has been previously shown that N/OFQ stimulates leukocyte chemotaxis both in vitro and in vivo. In the present study we investigated the ability of N/OFQ, in comparison with the proinflammatory peptide formyl-Met-Leu-Phe (fMLP), to stimulate human neutrophil and monocyte chemotaxis and the release of lysozyme and superoxide anion (O2-) production from neutrophils. fMLP stimulated all the leukocyte functions examined. N/OFQ stimulated monocyte (pEC50 12.15) but not neutrophil chemotaxis. The production of O2- from neutrophils was not affected by N/OFQ while the release of lysozyme was increased in a concentration dependent manner (pEC50 11.00) although the maximal effects evoked by N/OFQ were about half of those of fMLP. The NOP ligands [Arg14, Lys15]N/OFQ, N/OFQ(1-13)NH2, Ro 64-6198, UFP-101 and the opioid antagonist naloxone were used for pharmacologically characterizing the receptor involved in the monocyte chemoattractant action of N/OFQ. [Arg14, Lys15]N/OFQ, N/OFQ(1-13)NH2, and Ro 64-6198 mimicked the action of N/OFQ showing similar maximal effects and the following order of potency: [Arg14, Lys15]N/OFQ (pEC50 13.22)>Ro 64-6198 (pEC50 12.96)>N/OFQ(1-13)NH2 (pEC50 12.67)>N/OFQ (pEC50 12.15). Moreover, the monocyte chemoattractant action of N/OFQ was not modified by naloxone 1 microM while antagonized by UFP-101 10 microM (pA2 7.00). Thus, the order of potency of agonists and the antagonist selectivity demonstrated that N/OFQ stimulates human monocyte chemotaxis via NOP receptor activation. SN - 0196-9781 UR - https://www.unboundmedicine.com/medline/citation/15922491/Nociceptin/orphanin_FQ_stimulates_human_monocyte_chemotaxis_via_NOP_receptor_activation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(05)00133-6 DB - PRIME DP - Unbound Medicine ER -