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Localization of P2X and P2Y receptors in dorsal root ganglia of the cat.
J Histochem Cytochem 2005; 53(10):1273-82JH

Abstract

The distribution of P2X and P2Y receptor subtypes in upper lumbosacral cat dorsal root ganglia (DRG) has been investigated using immunohistochemistry. Intensity of immunoreactivity for six P2X receptors (P2X(5) receptors were immuno-negative) and the three P2Y receptors examined in cat DRG was in the order of P2Y(2) = P2Y(4)>P2X(3)>P2X(2) = P2X(7)>P2X(6)>P2X(1) = P2X(4)>P2Y(1). P2X(3), P2Y(2), and P2Y(4) receptor polyclonal antibodies stained 33.8%, 35.3%, and 47.6% of DRG neurons, respectively. Most P2Y(2), P2X(1), P2X(3), P2X(4), and P2X(6) receptor staining was detected in small- and medium-diameter neurons. However, P2Y(4), P2X(2), and P2X(7) staining was present in large- and small-diameter neurons. Double-labeling immunohistochemistry showed that 90.8%, 32.1%, and 2.4% of P2X(3) receptor-positive neurons coexpressed IB(4), CGRP, and NF200, respectively; whereas 67.4%, 41.3%, and 39.1% of P2Y(4) receptor-positive neurons coexpressed IB(4), CGRP, and NF200, respectively. A total of 18.8%, 16.6%, and 63.5% of P2Y(2) receptor-positive neurons also stained for IB(4), CGRP, and NF200, respectively. Only 30% of DRG neurons in cat were P2X(3)-immunoreactive compared with 90% in rat and in mouse. A further difference was the low expression of P2Y(1) receptors in cat DRG neurons compared with more than 80% of the neurons in rat. Many small-diameter neurons were NF200-positive in cat, again differing from rat and mouse.

Authors+Show Affiliations

Autonomic Neuroscience Centre, Royal Free & University College Medical School, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15923368

Citation

Ruan, Huai-Zhen, et al. "Localization of P2X and P2Y Receptors in Dorsal Root Ganglia of the Cat." The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, vol. 53, no. 10, 2005, pp. 1273-82.
Ruan HZ, Birder LA, de Groat WC, et al. Localization of P2X and P2Y receptors in dorsal root ganglia of the cat. J Histochem Cytochem. 2005;53(10):1273-82.
Ruan, H. Z., Birder, L. A., de Groat, W. C., Tai, C., Roppolo, J., Buffington, C. A., & Burnstock, G. (2005). Localization of P2X and P2Y receptors in dorsal root ganglia of the cat. The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society, 53(10), pp. 1273-82.
Ruan HZ, et al. Localization of P2X and P2Y Receptors in Dorsal Root Ganglia of the Cat. J Histochem Cytochem. 2005;53(10):1273-82. PubMed PMID: 15923368.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localization of P2X and P2Y receptors in dorsal root ganglia of the cat. AU - Ruan,Huai-Zhen, AU - Birder,Lori A, AU - de Groat,William C, AU - Tai,Changfeng, AU - Roppolo,James, AU - Buffington,Charles A, AU - Burnstock,Geoffrey, Y1 - 2005/05/27/ PY - 2005/6/1/pubmed PY - 2005/10/20/medline PY - 2005/6/1/entrez SP - 1273 EP - 82 JF - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society JO - J. Histochem. Cytochem. VL - 53 IS - 10 N2 - The distribution of P2X and P2Y receptor subtypes in upper lumbosacral cat dorsal root ganglia (DRG) has been investigated using immunohistochemistry. Intensity of immunoreactivity for six P2X receptors (P2X(5) receptors were immuno-negative) and the three P2Y receptors examined in cat DRG was in the order of P2Y(2) = P2Y(4)>P2X(3)>P2X(2) = P2X(7)>P2X(6)>P2X(1) = P2X(4)>P2Y(1). P2X(3), P2Y(2), and P2Y(4) receptor polyclonal antibodies stained 33.8%, 35.3%, and 47.6% of DRG neurons, respectively. Most P2Y(2), P2X(1), P2X(3), P2X(4), and P2X(6) receptor staining was detected in small- and medium-diameter neurons. However, P2Y(4), P2X(2), and P2X(7) staining was present in large- and small-diameter neurons. Double-labeling immunohistochemistry showed that 90.8%, 32.1%, and 2.4% of P2X(3) receptor-positive neurons coexpressed IB(4), CGRP, and NF200, respectively; whereas 67.4%, 41.3%, and 39.1% of P2Y(4) receptor-positive neurons coexpressed IB(4), CGRP, and NF200, respectively. A total of 18.8%, 16.6%, and 63.5% of P2Y(2) receptor-positive neurons also stained for IB(4), CGRP, and NF200, respectively. Only 30% of DRG neurons in cat were P2X(3)-immunoreactive compared with 90% in rat and in mouse. A further difference was the low expression of P2Y(1) receptors in cat DRG neurons compared with more than 80% of the neurons in rat. Many small-diameter neurons were NF200-positive in cat, again differing from rat and mouse. SN - 0022-1554 UR - https://www.unboundmedicine.com/medline/citation/15923368/Localization_of_P2X_and_P2Y_receptors_in_dorsal_root_ganglia_of_the_cat_ L2 - http://journals.sagepub.com/doi/full/10.1369/jhc.4A6556.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -