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Carbonylated proteins in bronchoalveolar lavage of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis and idiopathic pulmonary fibrosis.
Proteomics. 2005 Jul; 5(10):2612-8.P

Abstract

Oxygen-derived free radicals produced by phagocytes have been postulated to contribute to lung tissue damage occurring during diffuse lung diseases (DLD). The two-dimensional electrophoretic (2-DE) analysis of bronchoalveolar lavage (BAL) protein composition revealed different protein profiles in sarcoidosis (S), idiopathic pulmonary fibrosis (IPF) and systemic sclerosis (SSc) with a significant increase of low molecular weight proteins in IPF. Some of these proteins are involved in antioxidant processes. The aims of this report were to analyse the oxidative stress occurring in patients with DLD through determination of BAL protein carbonyl content and to identify target proteins of oxidation by a proteomic approach (2-DE combined with immunoblotting with specific antibodies for carbonyl groups). Carbonylated proteins detected by enzyme-linked immunosorbent assay (ELISA) were increased in BAL of patients with S, IPF and SSc compared to healthy controls with a significant difference for S and IPF. The proteomic approach to the analysis of BAL revealed that protein carbonylation was a process involving specific carbonylation-sensitive proteins and that in IPF a greater number of proteins target of oxidation were present. In conclusion, to our knowledge, this is the first report providing a database of proteins target of oxidation in BAL of patients with sarcoidosis, idiopathic pulmonary fibrosis and systemic sclerosis.

Authors+Show Affiliations

Respiratory Diseases Section, Department of Clinical Medicine and Immunological Sciences, University of Siena, Siena, Italy. rottoli@unisi.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15924291

Citation

Rottoli, Paola, et al. "Carbonylated Proteins in Bronchoalveolar Lavage of Patients With Sarcoidosis, Pulmonary Fibrosis Associated With Systemic Sclerosis and Idiopathic Pulmonary Fibrosis." Proteomics, vol. 5, no. 10, 2005, pp. 2612-8.
Rottoli P, Magi B, Cianti R, et al. Carbonylated proteins in bronchoalveolar lavage of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis and idiopathic pulmonary fibrosis. Proteomics. 2005;5(10):2612-8.
Rottoli, P., Magi, B., Cianti, R., Bargagli, E., Vagaggini, C., Nikiforakis, N., Pallini, V., & Bini, L. (2005). Carbonylated proteins in bronchoalveolar lavage of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis and idiopathic pulmonary fibrosis. Proteomics, 5(10), 2612-8.
Rottoli P, et al. Carbonylated Proteins in Bronchoalveolar Lavage of Patients With Sarcoidosis, Pulmonary Fibrosis Associated With Systemic Sclerosis and Idiopathic Pulmonary Fibrosis. Proteomics. 2005;5(10):2612-8. PubMed PMID: 15924291.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbonylated proteins in bronchoalveolar lavage of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis and idiopathic pulmonary fibrosis. AU - Rottoli,Paola, AU - Magi,Barbara, AU - Cianti,Riccardo, AU - Bargagli,Elena, AU - Vagaggini,Cecilia, AU - Nikiforakis,Nikolaos, AU - Pallini,Vitaliano, AU - Bini,Luca, PY - 2005/6/1/pubmed PY - 2005/10/5/medline PY - 2005/6/1/entrez SP - 2612 EP - 8 JF - Proteomics JO - Proteomics VL - 5 IS - 10 N2 - Oxygen-derived free radicals produced by phagocytes have been postulated to contribute to lung tissue damage occurring during diffuse lung diseases (DLD). The two-dimensional electrophoretic (2-DE) analysis of bronchoalveolar lavage (BAL) protein composition revealed different protein profiles in sarcoidosis (S), idiopathic pulmonary fibrosis (IPF) and systemic sclerosis (SSc) with a significant increase of low molecular weight proteins in IPF. Some of these proteins are involved in antioxidant processes. The aims of this report were to analyse the oxidative stress occurring in patients with DLD through determination of BAL protein carbonyl content and to identify target proteins of oxidation by a proteomic approach (2-DE combined with immunoblotting with specific antibodies for carbonyl groups). Carbonylated proteins detected by enzyme-linked immunosorbent assay (ELISA) were increased in BAL of patients with S, IPF and SSc compared to healthy controls with a significant difference for S and IPF. The proteomic approach to the analysis of BAL revealed that protein carbonylation was a process involving specific carbonylation-sensitive proteins and that in IPF a greater number of proteins target of oxidation were present. In conclusion, to our knowledge, this is the first report providing a database of proteins target of oxidation in BAL of patients with sarcoidosis, idiopathic pulmonary fibrosis and systemic sclerosis. SN - 1615-9853 UR - https://www.unboundmedicine.com/medline/citation/15924291/Carbonylated_proteins_in_bronchoalveolar_lavage_of_patients_with_sarcoidosis_pulmonary_fibrosis_associated_with_systemic_sclerosis_and_idiopathic_pulmonary_fibrosis_ L2 - https://doi.org/10.1002/pmic.200401206 DB - PRIME DP - Unbound Medicine ER -