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Localization of glycine receptor alpha subunits on bipolar and amacrine cells in primate retina.
J Comp Neurol 2005; 488(2):113-28JC

Abstract

The major inhibitory neurotransmitter glycine is used by about half of the amacrine cells in the retina. Amacrine cells provide synaptic output to bipolar, ganglion, and other amacrine cells. The present study investigated whether different bipolar and amacrine cell types in the primate retina differ with respect to the expression of glycine receptor (GlyR) subtypes. Antibodies specific for the alpha1, alpha2, and alpha3 subunits of the GlyR were combined with immunohistochemical markers for bipolar and amacrine cells and applied to vertical sections of macaque (Macaca fascicularis) and marmoset (Callithrix jacchus) retinae. For all subunits, punctate immunoreactivity was expressed in the inner plexiform layer. The GlyRalpha2 immunoreactive (IR) puncta occur at the highest density, followed by GlyR(alpha)3 and GlyR(alpha)1 IR puncta. Postembedding electron microscopy showed the postsynaptic location of all subunits. Double immunofluorescence demonstrated that the three alpha subunits are clustered at different postsynaptic sites. Two OFF cone bipolar cell types (flat midget and diffuse bipolar DB3), are predominantly associated with the alpha1 subunit. Two ON bipolar cell types, the DB6 and the rod bipolar cell, are predominantly associated with the alpha2 subunit. The glycinergic AII amacrine cell is presynaptic to the alpha1 subunit in the OFF-sublamina, and postsynaptic to the alpha2 subunit in the ON-sublamina. Another putative glycinergic cell, the vesicular glutamate transporter 3 cell, is predominantly presynaptic to the alpha2 subunit. The dopaminergic amacrine cell expresses the alpha3 subunit at a low density.

Authors+Show Affiliations

The National Vision Research Institute of Australia, Carlton, Victoria 3053, Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15924342

Citation

Jusuf, Patricia R., et al. "Localization of Glycine Receptor Alpha Subunits On Bipolar and Amacrine Cells in Primate Retina." The Journal of Comparative Neurology, vol. 488, no. 2, 2005, pp. 113-28.
Jusuf PR, Haverkamp S, Grünert U. Localization of glycine receptor alpha subunits on bipolar and amacrine cells in primate retina. J Comp Neurol. 2005;488(2):113-28.
Jusuf, P. R., Haverkamp, S., & Grünert, U. (2005). Localization of glycine receptor alpha subunits on bipolar and amacrine cells in primate retina. The Journal of Comparative Neurology, 488(2), pp. 113-28.
Jusuf PR, Haverkamp S, Grünert U. Localization of Glycine Receptor Alpha Subunits On Bipolar and Amacrine Cells in Primate Retina. J Comp Neurol. 2005 Jul 25;488(2):113-28. PubMed PMID: 15924342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localization of glycine receptor alpha subunits on bipolar and amacrine cells in primate retina. AU - Jusuf,Patricia R, AU - Haverkamp,Silke, AU - Grünert,Ulrike, PY - 2005/6/1/pubmed PY - 2005/9/1/medline PY - 2005/6/1/entrez SP - 113 EP - 28 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 488 IS - 2 N2 - The major inhibitory neurotransmitter glycine is used by about half of the amacrine cells in the retina. Amacrine cells provide synaptic output to bipolar, ganglion, and other amacrine cells. The present study investigated whether different bipolar and amacrine cell types in the primate retina differ with respect to the expression of glycine receptor (GlyR) subtypes. Antibodies specific for the alpha1, alpha2, and alpha3 subunits of the GlyR were combined with immunohistochemical markers for bipolar and amacrine cells and applied to vertical sections of macaque (Macaca fascicularis) and marmoset (Callithrix jacchus) retinae. For all subunits, punctate immunoreactivity was expressed in the inner plexiform layer. The GlyRalpha2 immunoreactive (IR) puncta occur at the highest density, followed by GlyR(alpha)3 and GlyR(alpha)1 IR puncta. Postembedding electron microscopy showed the postsynaptic location of all subunits. Double immunofluorescence demonstrated that the three alpha subunits are clustered at different postsynaptic sites. Two OFF cone bipolar cell types (flat midget and diffuse bipolar DB3), are predominantly associated with the alpha1 subunit. Two ON bipolar cell types, the DB6 and the rod bipolar cell, are predominantly associated with the alpha2 subunit. The glycinergic AII amacrine cell is presynaptic to the alpha1 subunit in the OFF-sublamina, and postsynaptic to the alpha2 subunit in the ON-sublamina. Another putative glycinergic cell, the vesicular glutamate transporter 3 cell, is predominantly presynaptic to the alpha2 subunit. The dopaminergic amacrine cell expresses the alpha3 subunit at a low density. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/15924342/Localization_of_glycine_receptor_alpha_subunits_on_bipolar_and_amacrine_cells_in_primate_retina_ L2 - https://doi.org/10.1002/cne.20555 DB - PRIME DP - Unbound Medicine ER -