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Effects of inactivation of the PPN1 gene on exopolyphosphatases, inorganic polyphosphates and function of mitochondria in the yeast Saccharomyces cerevisiae.
FEMS Yeast Res. 2005 Jun; 5(9):823-8.FY

Abstract

Mutants of Saccharomyces cerevisiae with inactivated endopolyphosphatase gene PPN1 did not grow on lactate and ethanol, and stopped growth on glucose earlier than the parent strain. Their mitochondria were defective in respiration functions and in metabolism of inorganic polyphosphates. The PPN1 mutants lacked exopolyphosphatase activity and possessed a double level of inorganic polyphosphates in mitochondria. The average chain length of mitochondrial polyphosphates at the stationary growth stage on glucose was about 15-20 and about 130-180 phosphate residues in the parent strain and PPN1 mutants, respectively. Inactivation of the PPX1 gene encoding exopolyphosphatase had no effect on respiration functions and on polyphosphate level and chain length in mitochondria.

Authors+Show Affiliations

Pestov NA
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region.
Kulakovskaya TV
No affiliation info available
Kulaev IS
No affiliation info available

MeSH

Acid Anhydride HydrolasesAdenosine TriphosphatasesElectrophoresis, Polyacrylamide GelEthanolGene Expression Regulation, FungalGene SilencingGlucoseLactic AcidMitochondriaPolyphosphatesSaccharomyces cerevisiae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15925310

Citation

Pestov, Nikolay A., et al. "Effects of Inactivation of the PPN1 Gene On Exopolyphosphatases, Inorganic Polyphosphates and Function of Mitochondria in the Yeast Saccharomyces Cerevisiae." FEMS Yeast Research, vol. 5, no. 9, 2005, pp. 823-8.
Pestov NA, Kulakovskaya TV, Kulaev IS. Effects of inactivation of the PPN1 gene on exopolyphosphatases, inorganic polyphosphates and function of mitochondria in the yeast Saccharomyces cerevisiae. FEMS Yeast Res. 2005;5(9):823-8.
Pestov, N. A., Kulakovskaya, T. V., & Kulaev, I. S. (2005). Effects of inactivation of the PPN1 gene on exopolyphosphatases, inorganic polyphosphates and function of mitochondria in the yeast Saccharomyces cerevisiae. FEMS Yeast Research, 5(9), 823-8.
Pestov NA, Kulakovskaya TV, Kulaev IS. Effects of Inactivation of the PPN1 Gene On Exopolyphosphatases, Inorganic Polyphosphates and Function of Mitochondria in the Yeast Saccharomyces Cerevisiae. FEMS Yeast Res. 2005;5(9):823-8. PubMed PMID: 15925310.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of inactivation of the PPN1 gene on exopolyphosphatases, inorganic polyphosphates and function of mitochondria in the yeast Saccharomyces cerevisiae. AU - Pestov,Nikolay A, AU - Kulakovskaya,Tatyana V, AU - Kulaev,Igor S, Y1 - 2005/03/30/ PY - 2004/10/10/received PY - 2005/02/18/revised PY - 2005/03/03/accepted PY - 2005/6/1/pubmed PY - 2005/8/3/medline PY - 2005/6/1/entrez SP - 823 EP - 8 JF - FEMS yeast research JO - FEMS Yeast Res VL - 5 IS - 9 N2 - Mutants of Saccharomyces cerevisiae with inactivated endopolyphosphatase gene PPN1 did not grow on lactate and ethanol, and stopped growth on glucose earlier than the parent strain. Their mitochondria were defective in respiration functions and in metabolism of inorganic polyphosphates. The PPN1 mutants lacked exopolyphosphatase activity and possessed a double level of inorganic polyphosphates in mitochondria. The average chain length of mitochondrial polyphosphates at the stationary growth stage on glucose was about 15-20 and about 130-180 phosphate residues in the parent strain and PPN1 mutants, respectively. Inactivation of the PPX1 gene encoding exopolyphosphatase had no effect on respiration functions and on polyphosphate level and chain length in mitochondria. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/15925310/Effects_of_inactivation_of_the_PPN1_gene_on_exopolyphosphatases_inorganic_polyphosphates_and_function_of_mitochondria_in_the_yeast_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -