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The minimum domain of Pho81 is not sufficient to control the Pho85-Rim15 effector branch involved in phosphate starvation-induced stress responses.
Curr Genet. 2005 Jul; 48(1):18-33.CG

Abstract

The phosphate regulatory mechanism in yeast, known as the PHO pathway, is regulated by inorganic phosphate to control the expression of genes involved in the acquisition of phosphate from the medium. This pathway is also reported to contribute to other nutritional responses and as such it affects several phenotypic characteristics known also to be regulated by protein kinase A, including the transcription of genes involved in the general stress response and trehalose metabolism. We now demonstrate that transcription of post-diauxic shift (PDS)-controlled stress-responsive genes is solely regulated by the Pho85-Pho80 complex, whereas regulation of trehalose metabolism apparently involves several Pho85 cyclins. Interestingly, both read-outs depend on Pho81 but, while the previously described minimum domain of Pho81 is sufficient to sustain phosphate-regulated transcription of PHO genes, full-length Pho81 is required to control trehalose metabolism and the PDS targets. Consistently, neither the expression control of stress-regulated genes nor the trehalose metabolism relies directly on Pho4. Finally, we present data supporting that the PHO pathway functions in parallel to the fermentable growth medium- or Sch9-controlled pathway and that both pathways may share the protein kinase Rim15, which was previously reported to play a central role in the integration of glucose, nitrogen and amino acid availability.

Authors+Show Affiliations

Swinnen E
Functional Biology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31, 3001 Heverlee, Belgium.
Rosseels J
No affiliation info available
Winderickx J
No affiliation info available

MeSH

Blotting, NorthernCulture MediaCyclin-Dependent KinasesCyclinsGene Expression Regulation, FungalGenome, FungalPhosphatesPlasmidsProtein KinasesRNA, FungalRepressor ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSignal TransductionTranscription, GeneticTrehalase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15926040

Citation

Swinnen, Erwin, et al. "The Minimum Domain of Pho81 Is Not Sufficient to Control the Pho85-Rim15 Effector Branch Involved in Phosphate Starvation-induced Stress Responses." Current Genetics, vol. 48, no. 1, 2005, pp. 18-33.
Swinnen E, Rosseels J, Winderickx J. The minimum domain of Pho81 is not sufficient to control the Pho85-Rim15 effector branch involved in phosphate starvation-induced stress responses. Curr Genet. 2005;48(1):18-33.
Swinnen, E., Rosseels, J., & Winderickx, J. (2005). The minimum domain of Pho81 is not sufficient to control the Pho85-Rim15 effector branch involved in phosphate starvation-induced stress responses. Current Genetics, 48(1), 18-33.
Swinnen E, Rosseels J, Winderickx J. The Minimum Domain of Pho81 Is Not Sufficient to Control the Pho85-Rim15 Effector Branch Involved in Phosphate Starvation-induced Stress Responses. Curr Genet. 2005;48(1):18-33. PubMed PMID: 15926040.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The minimum domain of Pho81 is not sufficient to control the Pho85-Rim15 effector branch involved in phosphate starvation-induced stress responses. AU - Swinnen,Erwin, AU - Rosseels,Joëlle, AU - Winderickx,Joris, Y1 - 2005/05/31/ PY - 2005/02/24/received PY - 2005/04/19/accepted PY - 2005/04/14/revised PY - 2005/6/1/pubmed PY - 2005/10/13/medline PY - 2005/6/1/entrez SP - 18 EP - 33 JF - Current genetics JO - Curr Genet VL - 48 IS - 1 N2 - The phosphate regulatory mechanism in yeast, known as the PHO pathway, is regulated by inorganic phosphate to control the expression of genes involved in the acquisition of phosphate from the medium. This pathway is also reported to contribute to other nutritional responses and as such it affects several phenotypic characteristics known also to be regulated by protein kinase A, including the transcription of genes involved in the general stress response and trehalose metabolism. We now demonstrate that transcription of post-diauxic shift (PDS)-controlled stress-responsive genes is solely regulated by the Pho85-Pho80 complex, whereas regulation of trehalose metabolism apparently involves several Pho85 cyclins. Interestingly, both read-outs depend on Pho81 but, while the previously described minimum domain of Pho81 is sufficient to sustain phosphate-regulated transcription of PHO genes, full-length Pho81 is required to control trehalose metabolism and the PDS targets. Consistently, neither the expression control of stress-regulated genes nor the trehalose metabolism relies directly on Pho4. Finally, we present data supporting that the PHO pathway functions in parallel to the fermentable growth medium- or Sch9-controlled pathway and that both pathways may share the protein kinase Rim15, which was previously reported to play a central role in the integration of glucose, nitrogen and amino acid availability. SN - 0172-8083 UR - https://www.unboundmedicine.com/medline/citation/15926040/The_minimum_domain_of_Pho81_is_not_sufficient_to_control_the_Pho85_Rim15_effector_branch_involved_in_phosphate_starvation_induced_stress_responses_ DB - PRIME DP - Unbound Medicine ER -