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Molecular cloning and characterization of two digestive serine proteases from the Hessian fly, Mayetiola destructor.
Insect Mol Biol. 2005 Jun; 14(3):309-18.IM

Abstract

Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Dipterans. Both mdesprot-I and mdesprot-II encoded proteins with secretion signal peptides at the N-terminals, indicating the proteins are secreted proteases that should function as midgut digestive proteases. A cytological analysis with fluorescent in situ hybridization revealed the cytological localization of mdesprot-I and mdesprot-II on the long arm of Autosome 2. Results are discussed in the context of the efficacy of potential protease inhibitors to develop Hessian fly resistant wheat through genetic engineering approaches.

Authors+Show Affiliations

Department of Entomology, Purdue University, West Lafayette, IN 47906, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15926900

Citation

Mittapalli, O, et al. "Molecular Cloning and Characterization of Two Digestive Serine Proteases From the Hessian Fly, Mayetiola Destructor." Insect Molecular Biology, vol. 14, no. 3, 2005, pp. 309-18.
Mittapalli O, Stuart JJ, Shukle RH. Molecular cloning and characterization of two digestive serine proteases from the Hessian fly, Mayetiola destructor. Insect Mol Biol. 2005;14(3):309-18.
Mittapalli, O., Stuart, J. J., & Shukle, R. H. (2005). Molecular cloning and characterization of two digestive serine proteases from the Hessian fly, Mayetiola destructor. Insect Molecular Biology, 14(3), 309-18.
Mittapalli O, Stuart JJ, Shukle RH. Molecular Cloning and Characterization of Two Digestive Serine Proteases From the Hessian Fly, Mayetiola Destructor. Insect Mol Biol. 2005;14(3):309-18. PubMed PMID: 15926900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and characterization of two digestive serine proteases from the Hessian fly, Mayetiola destructor. AU - Mittapalli,O, AU - Stuart,J J, AU - Shukle,R H, PY - 2005/6/2/pubmed PY - 2005/7/9/medline PY - 2005/6/2/entrez SP - 309 EP - 18 JF - Insect molecular biology JO - Insect Mol Biol VL - 14 IS - 3 N2 - Full-length cDNA and genomic sequences for two genes (designated mdesprot-I and mdesprot-II) encoding digestive serine proteases in Hessian fly, Mayetiola destructor, have been cloned and characterized. The deduced amino acid sequences revealed similarity with trypsin-like digestive serine proteases from other Dipterans. Both mdesprot-I and mdesprot-II encoded proteins with secretion signal peptides at the N-terminals, indicating the proteins are secreted proteases that should function as midgut digestive proteases. A cytological analysis with fluorescent in situ hybridization revealed the cytological localization of mdesprot-I and mdesprot-II on the long arm of Autosome 2. Results are discussed in the context of the efficacy of potential protease inhibitors to develop Hessian fly resistant wheat through genetic engineering approaches. SN - 0962-1075 UR - https://www.unboundmedicine.com/medline/citation/15926900/Molecular_cloning_and_characterization_of_two_digestive_serine_proteases_from_the_Hessian_fly_Mayetiola_destructor_ L2 - https://doi.org/10.1111/j.1365-2583.2005.00561.x DB - PRIME DP - Unbound Medicine ER -