Tags

Type your tag names separated by a space and hit enter

TGF-beta expression during rat pregnancy and activity on decidual cell survival.
Reprod Biol Endocrinol. 2005 May 31; 3:20.RB

Abstract

BACKGROUND

During early rat pregnancy, trophoblast of the tiny embryo joins with the endometrium and epithelial cells undergo apoptosis. Near the end of pregnancy, regression of the decidua basalis (DB) is also observed (from day 14 to 20). However, little is known about the intra-cellular and molecular mechanisms involved in apoptosis regulation in the uterus during pregnancy. The objective of the present study was to investigate the presence and the developmental expression of transforming growth factor-beta isoforms (TGF-beta well known differentiation factor) in the rat endometrium throughout pregnancy and its action in vitro using cultured endometrial stromal cells.

METHODS

In vivo: Rats were killed at different days of pregnancy (days 2-20) and uteri removed to collect endometrial protein extracts or the uteri were fixed, embedded and sectioned for immunohistochemistry (IHC) and in situ cell death analyses using TdT-mediated dUTP nick end labeling (TUNEL). In vitro: Rats were ovariectomized and decidualization was induced using sex steroids. Endometrial stromal decidual cells were then collected and cultured.

RESULTS

An increase of apoptosis in the DB on days 14, 16 and 18 was observed. Cleaved caspase-3 was clearly detected during regression of the DB by Western analysis and immunofluorescence. Western analyses using endometrial protein extracts demonstrated that TGF-beta1, TGF-beta2 and TGF-beta3 were highly expressed at the time of DB regression (day 14). During early pregnancy, TGF-beta1 and -beta2 expressions raised at days 5.5 to 6.5. TGF-beta3 protein was not detected during early pregnancy. IHC analyses revealed that TGF-beta1 and -2 were found surrounding both epithelium (luminal and glandular) in the stroma compartment at the implantation site, and TGF-beta3 was mainly located surrounding endometrial epithelium in the stroma compartment. Smad2 phosphorylation was increased at the time of DB regression. In vitro studies using decidual endometrial stromal cells revealed that TGF-beta1 induced apoptosis and Smad2 phosphorylation. Moreover, TGF-beta1 reduced both Akt (a well known survival factor) phosphorylation and XIAP (X-linked inhibitor of apoptosis protein) expression in decidual endometrial stromal cells in vitro.

CONCLUSION

Taken together, these results suggest that TGF-beta isoforms are regulated differently during pregnancy and may have an important role in the control of apoptosis and cell survival at specific stages during pregnancy.

Authors+Show Affiliations

Département de Chimie-Biologie, Université du Québec à Trois-Rivières, C.P. 500, Trois-Rivières, Québec, G9A 5H7, Canada. SHOONECA@CollegeSherbrooke.qc.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15927076

Citation

Shooner, Carl, et al. "TGF-beta Expression During Rat Pregnancy and Activity On Decidual Cell Survival." Reproductive Biology and Endocrinology : RB&E, vol. 3, 2005, p. 20.
Shooner C, Caron PL, Fréchette-Frigon G, et al. TGF-beta expression during rat pregnancy and activity on decidual cell survival. Reprod Biol Endocrinol. 2005;3:20.
Shooner, C., Caron, P. L., Fréchette-Frigon, G., Leblanc, V., Déry, M. C., & Asselin, E. (2005). TGF-beta expression during rat pregnancy and activity on decidual cell survival. Reproductive Biology and Endocrinology : RB&E, 3, 20.
Shooner C, et al. TGF-beta Expression During Rat Pregnancy and Activity On Decidual Cell Survival. Reprod Biol Endocrinol. 2005 May 31;3:20. PubMed PMID: 15927076.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TGF-beta expression during rat pregnancy and activity on decidual cell survival. AU - Shooner,Carl, AU - Caron,Pierre-Luc, AU - Fréchette-Frigon,Guylaine, AU - Leblanc,Valérie, AU - Déry,Marie-Claude, AU - Asselin,Eric, Y1 - 2005/05/31/ PY - 2005/04/27/received PY - 2005/05/31/accepted PY - 2005/6/2/pubmed PY - 2006/5/26/medline PY - 2005/6/2/entrez SP - 20 EP - 20 JF - Reproductive biology and endocrinology : RB&E JO - Reprod. Biol. Endocrinol. VL - 3 N2 - BACKGROUND: During early rat pregnancy, trophoblast of the tiny embryo joins with the endometrium and epithelial cells undergo apoptosis. Near the end of pregnancy, regression of the decidua basalis (DB) is also observed (from day 14 to 20). However, little is known about the intra-cellular and molecular mechanisms involved in apoptosis regulation in the uterus during pregnancy. The objective of the present study was to investigate the presence and the developmental expression of transforming growth factor-beta isoforms (TGF-beta well known differentiation factor) in the rat endometrium throughout pregnancy and its action in vitro using cultured endometrial stromal cells. METHODS: In vivo: Rats were killed at different days of pregnancy (days 2-20) and uteri removed to collect endometrial protein extracts or the uteri were fixed, embedded and sectioned for immunohistochemistry (IHC) and in situ cell death analyses using TdT-mediated dUTP nick end labeling (TUNEL). In vitro: Rats were ovariectomized and decidualization was induced using sex steroids. Endometrial stromal decidual cells were then collected and cultured. RESULTS: An increase of apoptosis in the DB on days 14, 16 and 18 was observed. Cleaved caspase-3 was clearly detected during regression of the DB by Western analysis and immunofluorescence. Western analyses using endometrial protein extracts demonstrated that TGF-beta1, TGF-beta2 and TGF-beta3 were highly expressed at the time of DB regression (day 14). During early pregnancy, TGF-beta1 and -beta2 expressions raised at days 5.5 to 6.5. TGF-beta3 protein was not detected during early pregnancy. IHC analyses revealed that TGF-beta1 and -2 were found surrounding both epithelium (luminal and glandular) in the stroma compartment at the implantation site, and TGF-beta3 was mainly located surrounding endometrial epithelium in the stroma compartment. Smad2 phosphorylation was increased at the time of DB regression. In vitro studies using decidual endometrial stromal cells revealed that TGF-beta1 induced apoptosis and Smad2 phosphorylation. Moreover, TGF-beta1 reduced both Akt (a well known survival factor) phosphorylation and XIAP (X-linked inhibitor of apoptosis protein) expression in decidual endometrial stromal cells in vitro. CONCLUSION: Taken together, these results suggest that TGF-beta isoforms are regulated differently during pregnancy and may have an important role in the control of apoptosis and cell survival at specific stages during pregnancy. SN - 1477-7827 UR - https://www.unboundmedicine.com/medline/citation/15927076/TGF_beta_expression_during_rat_pregnancy_and_activity_on_decidual_cell_survival_ L2 - https://rbej.biomedcentral.com/articles/10.1186/1477-7827-3-20 DB - PRIME DP - Unbound Medicine ER -