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The oxidant and antioxidant effects of 25-hydroxyvitamin D3 in liver, kidney and heart tissues of diabetic rats.
Clin Exp Med. 2005 May; 5(1):31-6.CE

Abstract

Previous studies have implicated protective effects of vitamin D on insulin secretion and pancreas beta cell function. The goal of the present study is to determine if a combination therapy of 25-hydroxyvitamin D3 and insulin had any advantage over insulin therapy alone on lipid peroxidation and antioxidant activity in the streptozotocin (STZ)-induced diabetic rat. The lipid peroxidation product, thiobarbituric acid-reacting substances (TBARS), was measured to assess free radical activity in the heart, kidney and liver tissues. The enzymatic activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) were measured as indicators of antioxidation in these tissues. Sprague-Dawley rats were made diabetic with a single injection of STZ (75 mg/kg i.p.). Rats were separated into three groups, each containing 10 animals: Group 1, non-diabetic and no drug treatment was given; Group 2, diabetic rats were treated with 3 IU/day subcutaneous (s.c.) insulin; and Group 3, diabetic rats were treated with 3 IU/day (s.c.) insulin plus 1 mg/kg/day per oral (p.o.) 25-hydroxyvitamin D3 for a period of 4 weeks. At the end of the study, TBARS contents of the liver, kidney and heart tissues in Groups 2 and 3 were found to be significantly increased as compared to Group 1 (P<0.05) and kidney MDA levels in Group 3 were also significantly increased as compared to Group 2 (P<0.05). The SOD and CAT contents of the heart in Group 2 were significantly increased as compared to Groups 1 and 3 (P<0.05). GSH-Px activity was unaltered in all groups (P>0.05). We suggest that a combination of insulin with 25-hydroxyvitamin D3 treatment would not be more beneficial than the use of insulin alone in antioxidant defence of diabetic liver and kidney tissues.

Authors+Show Affiliations

Department of Biochemistry and Clinical Biochemistry, School of Medicine, Yuzuncu Yil University, Van, Turkey. tnoyan@yyu.edu.trNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15928880

Citation

Noyan, T, et al. "The Oxidant and Antioxidant Effects of 25-hydroxyvitamin D3 in Liver, Kidney and Heart Tissues of Diabetic Rats." Clinical and Experimental Medicine, vol. 5, no. 1, 2005, pp. 31-6.
Noyan T, Balaharoğlu R, Kömüroğlu U. The oxidant and antioxidant effects of 25-hydroxyvitamin D3 in liver, kidney and heart tissues of diabetic rats. Clin Exp Med. 2005;5(1):31-6.
Noyan, T., Balaharoğlu, R., & Kömüroğlu, U. (2005). The oxidant and antioxidant effects of 25-hydroxyvitamin D3 in liver, kidney and heart tissues of diabetic rats. Clinical and Experimental Medicine, 5(1), 31-6.
Noyan T, Balaharoğlu R, Kömüroğlu U. The Oxidant and Antioxidant Effects of 25-hydroxyvitamin D3 in Liver, Kidney and Heart Tissues of Diabetic Rats. Clin Exp Med. 2005;5(1):31-6. PubMed PMID: 15928880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The oxidant and antioxidant effects of 25-hydroxyvitamin D3 in liver, kidney and heart tissues of diabetic rats. AU - Noyan,T, AU - Balaharoğlu,R, AU - Kömüroğlu,U, PY - 2004/10/19/received PY - 2005/03/16/accepted PY - 2005/6/2/pubmed PY - 2005/11/3/medline PY - 2005/6/2/entrez SP - 31 EP - 6 JF - Clinical and experimental medicine JO - Clin Exp Med VL - 5 IS - 1 N2 - Previous studies have implicated protective effects of vitamin D on insulin secretion and pancreas beta cell function. The goal of the present study is to determine if a combination therapy of 25-hydroxyvitamin D3 and insulin had any advantage over insulin therapy alone on lipid peroxidation and antioxidant activity in the streptozotocin (STZ)-induced diabetic rat. The lipid peroxidation product, thiobarbituric acid-reacting substances (TBARS), was measured to assess free radical activity in the heart, kidney and liver tissues. The enzymatic activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) were measured as indicators of antioxidation in these tissues. Sprague-Dawley rats were made diabetic with a single injection of STZ (75 mg/kg i.p.). Rats were separated into three groups, each containing 10 animals: Group 1, non-diabetic and no drug treatment was given; Group 2, diabetic rats were treated with 3 IU/day subcutaneous (s.c.) insulin; and Group 3, diabetic rats were treated with 3 IU/day (s.c.) insulin plus 1 mg/kg/day per oral (p.o.) 25-hydroxyvitamin D3 for a period of 4 weeks. At the end of the study, TBARS contents of the liver, kidney and heart tissues in Groups 2 and 3 were found to be significantly increased as compared to Group 1 (P<0.05) and kidney MDA levels in Group 3 were also significantly increased as compared to Group 2 (P<0.05). The SOD and CAT contents of the heart in Group 2 were significantly increased as compared to Groups 1 and 3 (P<0.05). GSH-Px activity was unaltered in all groups (P>0.05). We suggest that a combination of insulin with 25-hydroxyvitamin D3 treatment would not be more beneficial than the use of insulin alone in antioxidant defence of diabetic liver and kidney tissues. SN - 1591-8890 UR - https://www.unboundmedicine.com/medline/citation/15928880/The_oxidant_and_antioxidant_effects_of_25_hydroxyvitamin_D3_in_liver_kidney_and_heart_tissues_of_diabetic_rats_ L2 - https://dx.doi.org/10.1007/s10238-005-0061-8 DB - PRIME DP - Unbound Medicine ER -