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Intestinal renal metabolism of L-citrulline and L-arginine following enteral or parenteral infusion of L-alanyl-L-[2,15N]glutamine or L-[2,15N]glutamine in mice.

Abstract

Previously, we observed increased plasma arginine (ARG) concentrations after glutamine (GLN)-enriched diets, in combination with clinical benefits. GLN delivers nitrogen for ARG synthesis, and the present study was designed to quantify the interorgan relationship of exogenous L-GLN or GLN dipeptide, by enteral or parenteral route, contributing to intestinal citrulline (CIT) and renal de novo ARG synthesis in mice. To study this, we used a multicatheterized mouse model with Swiss mice (n = 43) in the postabsorptive state. Stable isotopes were infused into the jugular vein or into the duodenum {per group either free L-[2,(15)N]GLN or dipeptide L-ALA-L-[2,(15)N]GLN, all with L-[ureido-(13)C-(2)H(2)]CIT and L-[guanidino-(15)N(2)-(2)H(2)]ARG} to establish renal and intestinal ARG and CIT metabolism. Blood flow was measured using (14)C-para-aminohippuric acid. Net intestinal CIT release, renal uptake of CIT, and net renal ARG efflux was found, as assessed by arteriovenous flux measurements. Quantitatively, more de novo L-[2,(15)N]CIT was produced when free L-[2,(15)N]GLN was given than when L-ALA-L-[2,(15)N]GLN was given, whereas renal de novo L-[2,(15)N]ARG was similar in all groups. In conclusion, the intestinal-renal axis is hereby proven in mice in that L-[2,(15)N]GLN or dipeptide were both converted into de novo renal L-[2,(15)N]ARG; however, not all was derived from intestinal L-[2,(15)N]CIT production. In this model, the feeding route and form of GLN did not influence de novo renal ARG production derived from GLN.

Authors+Show Affiliations

Dept. of Surgery, Vrije Universiteit University Medical Center, 1007 MB Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15933220

Citation

Boelens, Petra G., et al. "Intestinal Renal Metabolism of L-citrulline and L-arginine Following Enteral or Parenteral Infusion of L-alanyl-L-[2,15N]glutamine or L-[2,15N]glutamine in Mice." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 289, no. 4, 2005, pp. G679-85.
Boelens PG, van Leeuwen PA, Dejong CH, et al. Intestinal renal metabolism of L-citrulline and L-arginine following enteral or parenteral infusion of L-alanyl-L-[2,15N]glutamine or L-[2,15N]glutamine in mice. Am J Physiol Gastrointest Liver Physiol. 2005;289(4):G679-85.
Boelens, P. G., van Leeuwen, P. A., Dejong, C. H., & Deutz, N. E. (2005). Intestinal renal metabolism of L-citrulline and L-arginine following enteral or parenteral infusion of L-alanyl-L-[2,15N]glutamine or L-[2,15N]glutamine in mice. American Journal of Physiology. Gastrointestinal and Liver Physiology, 289(4), pp. G679-85.
Boelens PG, et al. Intestinal Renal Metabolism of L-citrulline and L-arginine Following Enteral or Parenteral Infusion of L-alanyl-L-[2,15N]glutamine or L-[2,15N]glutamine in Mice. Am J Physiol Gastrointest Liver Physiol. 2005;289(4):G679-85. PubMed PMID: 15933220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intestinal renal metabolism of L-citrulline and L-arginine following enteral or parenteral infusion of L-alanyl-L-[2,15N]glutamine or L-[2,15N]glutamine in mice. AU - Boelens,Petra G, AU - van Leeuwen,Paul A M, AU - Dejong,Cornelis H C, AU - Deutz,Nicolaas E P, Y1 - 2005/06/02/ PY - 2005/6/4/pubmed PY - 2005/10/28/medline PY - 2005/6/4/entrez SP - G679 EP - 85 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am. J. Physiol. Gastrointest. Liver Physiol. VL - 289 IS - 4 N2 - Previously, we observed increased plasma arginine (ARG) concentrations after glutamine (GLN)-enriched diets, in combination with clinical benefits. GLN delivers nitrogen for ARG synthesis, and the present study was designed to quantify the interorgan relationship of exogenous L-GLN or GLN dipeptide, by enteral or parenteral route, contributing to intestinal citrulline (CIT) and renal de novo ARG synthesis in mice. To study this, we used a multicatheterized mouse model with Swiss mice (n = 43) in the postabsorptive state. Stable isotopes were infused into the jugular vein or into the duodenum {per group either free L-[2,(15)N]GLN or dipeptide L-ALA-L-[2,(15)N]GLN, all with L-[ureido-(13)C-(2)H(2)]CIT and L-[guanidino-(15)N(2)-(2)H(2)]ARG} to establish renal and intestinal ARG and CIT metabolism. Blood flow was measured using (14)C-para-aminohippuric acid. Net intestinal CIT release, renal uptake of CIT, and net renal ARG efflux was found, as assessed by arteriovenous flux measurements. Quantitatively, more de novo L-[2,(15)N]CIT was produced when free L-[2,(15)N]GLN was given than when L-ALA-L-[2,(15)N]GLN was given, whereas renal de novo L-[2,(15)N]ARG was similar in all groups. In conclusion, the intestinal-renal axis is hereby proven in mice in that L-[2,(15)N]GLN or dipeptide were both converted into de novo renal L-[2,(15)N]ARG; however, not all was derived from intestinal L-[2,(15)N]CIT production. In this model, the feeding route and form of GLN did not influence de novo renal ARG production derived from GLN. SN - 0193-1857 UR - https://www.unboundmedicine.com/medline/citation/15933220/Intestinal_renal_metabolism_of_L_citrulline_and_L_arginine_following_enteral_or_parenteral_infusion_of_L_alanyl_L_[215N]glutamine_or_L_[215N]glutamine_in_mice_ L2 - http://www.physiology.org/doi/full/10.1152/ajpgi.00026.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -