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Gene--nutrition interactions in coronary artery disease: correlation between the MTHFR C677T polymorphism and folate and homocysteine status in a Korean population.
Thromb Res 2006; 117(5):501-6TR

Abstract

INTRODUCTION

Elevated plasma total homocysteine is a major risk for coronary artery disease (CAD). Methyltetrahydrofolate reductase (MTHFR) is a main regulatory enzyme in homocysteine metabolism; a common C677T mutation in the MTHFR gene results in decreased enzyme activity, and contributes to increased homocysteine levels and decreased folate levels. We investigated the frequency of MTHFR C677T alleles in a Korean population, determined the genotype-specific threshold levels of folate or vitamin B12, and investigated the relationship between the TT genotype and the risk of CAD.

MATERIALS AND METHODS

We enrolled a study population of 163 CAD patients and 50 control subjects, and screened the MTHFR C677T polymorphism using real-time PCR with melting point analysis. Levels of plasma homocysteine, folate and vitamin B12 were also determined. We then defined the genotype-specific threshold values of folate and vitamin B12 required to keep homocysteine levels in a normal range for individuals of each MTHFR C677T genotype.

RESULTS

The frequency of the TT genotype was 18% in control subjects and 26% in patients group (P>0.05). Individuals homozygous for the TT genotype had significantly elevated homocysteine levels (P<0.05). The genotype-specific folate threshold level was significantly higher in TT individuals than in the CC or CT genotypes. The OR of individuals with low folate status and the TT genotype to estimate the relative risk of CAD was 2.2 and the OR of those with high folate status and the TT genotype was 1.5 (95% CI, 0.5-9.6 and 0.7-3.2, respectively).

CONCLUSION

We were able to define a gene-nutrient interaction that shows a higher risk for CAD based on specific threshold folate levels required by different MTHFR C677T genotypes in a Korean population.

Authors+Show Affiliations

Department of Laboratory Medicine, College of Medicine, University of Ulsan and Asan Medical Center, 388-1 Pungnap-dong, Songpa-gu, 138-736 Seoul, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15935452

Citation

Huh, Hee Jin, et al. "Gene--nutrition Interactions in Coronary Artery Disease: Correlation Between the MTHFR C677T Polymorphism and Folate and Homocysteine Status in a Korean Population." Thrombosis Research, vol. 117, no. 5, 2006, pp. 501-6.
Huh HJ, Chi HS, Shim EH, et al. Gene--nutrition interactions in coronary artery disease: correlation between the MTHFR C677T polymorphism and folate and homocysteine status in a Korean population. Thromb Res. 2006;117(5):501-6.
Huh, H. J., Chi, H. S., Shim, E. H., Jang, S., & Park, C. J. (2006). Gene--nutrition interactions in coronary artery disease: correlation between the MTHFR C677T polymorphism and folate and homocysteine status in a Korean population. Thrombosis Research, 117(5), pp. 501-6.
Huh HJ, et al. Gene--nutrition Interactions in Coronary Artery Disease: Correlation Between the MTHFR C677T Polymorphism and Folate and Homocysteine Status in a Korean Population. Thromb Res. 2006;117(5):501-6. PubMed PMID: 15935452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene--nutrition interactions in coronary artery disease: correlation between the MTHFR C677T polymorphism and folate and homocysteine status in a Korean population. AU - Huh,Hee Jin, AU - Chi,Hyun Sook, AU - Shim,Eun Hee, AU - Jang,Seongsoo, AU - Park,Chan Jeoung, Y1 - 2005/06/01/ PY - 2004/12/22/received PY - 2005/04/03/revised PY - 2005/04/07/accepted PY - 2005/6/7/pubmed PY - 2006/9/20/medline PY - 2005/6/7/entrez SP - 501 EP - 6 JF - Thrombosis research JO - Thromb. Res. VL - 117 IS - 5 N2 - INTRODUCTION: Elevated plasma total homocysteine is a major risk for coronary artery disease (CAD). Methyltetrahydrofolate reductase (MTHFR) is a main regulatory enzyme in homocysteine metabolism; a common C677T mutation in the MTHFR gene results in decreased enzyme activity, and contributes to increased homocysteine levels and decreased folate levels. We investigated the frequency of MTHFR C677T alleles in a Korean population, determined the genotype-specific threshold levels of folate or vitamin B12, and investigated the relationship between the TT genotype and the risk of CAD. MATERIALS AND METHODS: We enrolled a study population of 163 CAD patients and 50 control subjects, and screened the MTHFR C677T polymorphism using real-time PCR with melting point analysis. Levels of plasma homocysteine, folate and vitamin B12 were also determined. We then defined the genotype-specific threshold values of folate and vitamin B12 required to keep homocysteine levels in a normal range for individuals of each MTHFR C677T genotype. RESULTS: The frequency of the TT genotype was 18% in control subjects and 26% in patients group (P>0.05). Individuals homozygous for the TT genotype had significantly elevated homocysteine levels (P<0.05). The genotype-specific folate threshold level was significantly higher in TT individuals than in the CC or CT genotypes. The OR of individuals with low folate status and the TT genotype to estimate the relative risk of CAD was 2.2 and the OR of those with high folate status and the TT genotype was 1.5 (95% CI, 0.5-9.6 and 0.7-3.2, respectively). CONCLUSION: We were able to define a gene-nutrient interaction that shows a higher risk for CAD based on specific threshold folate levels required by different MTHFR C677T genotypes in a Korean population. SN - 0049-3848 UR - https://www.unboundmedicine.com/medline/citation/15935452/Gene__nutrition_interactions_in_coronary_artery_disease:_correlation_between_the_MTHFR_C677T_polymorphism_and_folate_and_homocysteine_status_in_a_Korean_population_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0049-3848(05)00167-2 DB - PRIME DP - Unbound Medicine ER -