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Evaluation of mutagenic and antimutagenic activities of alpha-bisabolol in the Salmonella/microsome assay.
Mutat Res. 2005 Aug 01; 585(1-2):105-12.MR

Abstract

alpha-Bisabolol (BISA) is a sesquiterpene alcohol found in the oils of chamomile (Matricaria chamomilla) and other plants. BISA has been widely used in dermatological and cosmetic formulations. This study was undertaken to investigate the mutagenicity and antimutagenicity of BISA in the Salmonella/microsome assay. Mutagenicity of BISA was evaluated with TA100, TA98, TA97a and TA1535 Salmonella typhimurium strains, without and with addition of S9 mixture. No increase in the number of his+ revertant colonies over the negative (solvent) control values was observed with any of the four tester strains. In the antimutagenicity assays, BISA was tested up to the highest nontoxic dose (i.e. 50 and 150 microg/plate, with and without S9 mix, respectively) against direct-acting (sodium azide, SA; 4-nitroquinoline-N-oxide, 4-NQNO; 2-nitrofluorene, 2-NF; and nitro-o-phenylenediamine, NPD) as well as indirect-acting (cyclophosphamide, CP; benzo[a]pyrene, B[a]P; aflatoxin B1, AFB1; 2-aminoanthracene, 2-AA; and 2-aminofluorene, 2-AF) mutagens. BISA did not alter mutagenic activity of SA and of NPD, and showed only a weak inhibitory effect on the mutagenicity induced by 4-NQNO and 2-NF. The mutagenic effects of AFB1, CP, B[a]P, 2-AA and 2-AF, on the other hand, were all markedly and dose-dependently reduced by BISA. It was also found that BISA inhibited pentoxyresorufin-o-depentylase (PROD, IC50 2.76 microM) and ethoxyresorufin-o-deethylase (EROD, 33.67 microM), which are markers for cytochromes CYP2B1 and 1A1 in rat liver microsomes. Since CYP2B1 converts AFB1 and CP into mutagenic metabolites, and CYP1A1 activates B[a]P, 2-AA and 2-AF, results suggest that BISA-induced antimutagenicity could be mediated by an inhibitory effect on the metabolic activation of these promutagens.

Authors+Show Affiliations

Gomes-Carneiro MR
Laboratory of Environmental Toxicology, Department of Biological Sciences, National School of Public Health, Oswaldo Cruz Foundation, Av. Brasil 4036, Rio de Janeiro, RJ 21040-361, Brazi1.
Dias DM
No affiliation info available
De-Oliveira AC
No affiliation info available
Paumgartten FJ
No affiliation info available

MeSH

AnimalsAntimutagenic AgentsAryl Hydrocarbon HydroxylasesCytochrome P-450 CYP1A1Cytochrome P-450 CYP2B1Dose-Response Relationship, DrugEnzyme InhibitorsFemaleMicrosomes, LiverMonocyclic SesquiterpenesMutagenicity TestsRatsRats, WistarSalmonella typhimuriumSesquiterpenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15936245

Citation

Gomes-Carneiro, M R., et al. "Evaluation of Mutagenic and Antimutagenic Activities of Alpha-bisabolol in the Salmonella/microsome Assay." Mutation Research, vol. 585, no. 1-2, 2005, pp. 105-12.
Gomes-Carneiro MR, Dias DM, De-Oliveira AC, et al. Evaluation of mutagenic and antimutagenic activities of alpha-bisabolol in the Salmonella/microsome assay. Mutat Res. 2005;585(1-2):105-12.
Gomes-Carneiro, M. R., Dias, D. M., De-Oliveira, A. C., & Paumgartten, F. J. (2005). Evaluation of mutagenic and antimutagenic activities of alpha-bisabolol in the Salmonella/microsome assay. Mutation Research, 585(1-2), 105-12.
Gomes-Carneiro MR, et al. Evaluation of Mutagenic and Antimutagenic Activities of Alpha-bisabolol in the Salmonella/microsome Assay. Mutat Res. 2005 Aug 1;585(1-2):105-12. PubMed PMID: 15936245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of mutagenic and antimutagenic activities of alpha-bisabolol in the Salmonella/microsome assay. AU - Gomes-Carneiro,M R, AU - Dias,Daniela M M, AU - De-Oliveira,A C A X, AU - Paumgartten,Francisco J R, PY - 2005/01/08/received PY - 2005/04/18/revised PY - 2005/04/22/accepted PY - 2005/6/7/pubmed PY - 2005/9/2/medline PY - 2005/6/7/entrez SP - 105 EP - 12 JF - Mutation research JO - Mutat Res VL - 585 IS - 1-2 N2 - alpha-Bisabolol (BISA) is a sesquiterpene alcohol found in the oils of chamomile (Matricaria chamomilla) and other plants. BISA has been widely used in dermatological and cosmetic formulations. This study was undertaken to investigate the mutagenicity and antimutagenicity of BISA in the Salmonella/microsome assay. Mutagenicity of BISA was evaluated with TA100, TA98, TA97a and TA1535 Salmonella typhimurium strains, without and with addition of S9 mixture. No increase in the number of his+ revertant colonies over the negative (solvent) control values was observed with any of the four tester strains. In the antimutagenicity assays, BISA was tested up to the highest nontoxic dose (i.e. 50 and 150 microg/plate, with and without S9 mix, respectively) against direct-acting (sodium azide, SA; 4-nitroquinoline-N-oxide, 4-NQNO; 2-nitrofluorene, 2-NF; and nitro-o-phenylenediamine, NPD) as well as indirect-acting (cyclophosphamide, CP; benzo[a]pyrene, B[a]P; aflatoxin B1, AFB1; 2-aminoanthracene, 2-AA; and 2-aminofluorene, 2-AF) mutagens. BISA did not alter mutagenic activity of SA and of NPD, and showed only a weak inhibitory effect on the mutagenicity induced by 4-NQNO and 2-NF. The mutagenic effects of AFB1, CP, B[a]P, 2-AA and 2-AF, on the other hand, were all markedly and dose-dependently reduced by BISA. It was also found that BISA inhibited pentoxyresorufin-o-depentylase (PROD, IC50 2.76 microM) and ethoxyresorufin-o-deethylase (EROD, 33.67 microM), which are markers for cytochromes CYP2B1 and 1A1 in rat liver microsomes. Since CYP2B1 converts AFB1 and CP into mutagenic metabolites, and CYP1A1 activates B[a]P, 2-AA and 2-AF, results suggest that BISA-induced antimutagenicity could be mediated by an inhibitory effect on the metabolic activation of these promutagens. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/15936245/Evaluation_of_mutagenic_and_antimutagenic_activities_of_alpha_bisabolol_in_the_Salmonella/microsome_assay_ DB - PRIME DP - Unbound Medicine ER -