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Products by the sphingosine kinase/sphingosine 1-phosphate (S1P) lyase pathway but not S1P stimulate mitogenesis.
Genes Cells 2005; 10(6):605-15GC

Abstract

Sphingosine 1-phosphate (S1P) functions as a ligand for the S1P/EDG family receptors. For years, intracellular signaling roles for S1P have also been suggested, especially in cell proliferation. Now, we have generated several mouse F9 embryonic carcinoma cell lines varying in expression of the S1P-degrading enzyme, S1P lyase (SPL) and/or sphingosine kinase (SPHK1). All these cell lines accumulated S1P compared to the wild-type F9 cells, but the amounts varied. We investigated the ability of these cells to proliferate under low serum conditions, as measured by a thymidine uptake assay. Although F9 cells over-expressing SPHK1 did exhibit enhanced DNA synthesis, other S1P-accumulating cells (SPL-null cells and SPL-null cells over-expressing SPHK1) did not. The overproduction of both SPL and SPHK1 resulted in the most striking mitogenic effect. Moreover, nM concentrations of sphingosine (or dihydrosphingosine) stimulated DNA synthesis in an SPL-dependent manner. These results indicate that products by the SPL pathway, not S1P itself, function in mitogenesis.

Authors+Show Affiliations

Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12-jo, Nishi 6-choume, Kita-ku, Sapporo 060-0812, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15938718

Citation

Kariya, Yuki, et al. "Products By the Sphingosine Kinase/sphingosine 1-phosphate (S1P) Lyase Pathway but Not S1P Stimulate Mitogenesis." Genes to Cells : Devoted to Molecular & Cellular Mechanisms, vol. 10, no. 6, 2005, pp. 605-15.
Kariya Y, Kihara A, Ikeda M, et al. Products by the sphingosine kinase/sphingosine 1-phosphate (S1P) lyase pathway but not S1P stimulate mitogenesis. Genes Cells. 2005;10(6):605-15.
Kariya, Y., Kihara, A., Ikeda, M., Kikuchi, F., Nakamura, S., Hashimoto, S., ... Igarashi, Y. (2005). Products by the sphingosine kinase/sphingosine 1-phosphate (S1P) lyase pathway but not S1P stimulate mitogenesis. Genes to Cells : Devoted to Molecular & Cellular Mechanisms, 10(6), pp. 605-15.
Kariya Y, et al. Products By the Sphingosine Kinase/sphingosine 1-phosphate (S1P) Lyase Pathway but Not S1P Stimulate Mitogenesis. Genes Cells. 2005;10(6):605-15. PubMed PMID: 15938718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Products by the sphingosine kinase/sphingosine 1-phosphate (S1P) lyase pathway but not S1P stimulate mitogenesis. AU - Kariya,Yuki, AU - Kihara,Akio, AU - Ikeda,Mika, AU - Kikuchi,Fumiaki, AU - Nakamura,Seiichi, AU - Hashimoto,Shunichi, AU - Choi,Chang-Hwan, AU - Lee,Yong-Moon, AU - Igarashi,Yasuyuki, PY - 2005/6/9/pubmed PY - 2005/8/27/medline PY - 2005/6/9/entrez SP - 605 EP - 15 JF - Genes to cells : devoted to molecular & cellular mechanisms JO - Genes Cells VL - 10 IS - 6 N2 - Sphingosine 1-phosphate (S1P) functions as a ligand for the S1P/EDG family receptors. For years, intracellular signaling roles for S1P have also been suggested, especially in cell proliferation. Now, we have generated several mouse F9 embryonic carcinoma cell lines varying in expression of the S1P-degrading enzyme, S1P lyase (SPL) and/or sphingosine kinase (SPHK1). All these cell lines accumulated S1P compared to the wild-type F9 cells, but the amounts varied. We investigated the ability of these cells to proliferate under low serum conditions, as measured by a thymidine uptake assay. Although F9 cells over-expressing SPHK1 did exhibit enhanced DNA synthesis, other S1P-accumulating cells (SPL-null cells and SPL-null cells over-expressing SPHK1) did not. The overproduction of both SPL and SPHK1 resulted in the most striking mitogenic effect. Moreover, nM concentrations of sphingosine (or dihydrosphingosine) stimulated DNA synthesis in an SPL-dependent manner. These results indicate that products by the SPL pathway, not S1P itself, function in mitogenesis. SN - 1356-9597 UR - https://www.unboundmedicine.com/medline/citation/15938718/Products_by_the_sphingosine_kinase/sphingosine_1_phosphate__S1P__lyase_pathway_but_not_S1P_stimulate_mitogenesis_ L2 - https://doi.org/10.1111/j.1365-2443.2005.00862.x DB - PRIME DP - Unbound Medicine ER -