TY - JOUR T1 - Adoptive immunotherapy of feline immunodeficiency virus with autologous ex vivo-stimulated lymphoid cells modulates virus and T-cell subsets in blood. AU - Flynn,J Norman, AU - Pistello,Mauro, AU - Isola,Patrizia, AU - Zaccaro,Lucia, AU - Del Santo,Barbara, AU - Ricci,Enrica, AU - Matteucci,Donatella, AU - Bendinelli,Mauro, PY - 2005/6/9/pubmed PY - 2006/4/13/medline PY - 2005/6/9/entrez SP - 736 EP - 45 JF - Clinical and diagnostic laboratory immunology JO - Clin Diagn Lab Immunol VL - 12 IS - 6 N2 - The potential of immunotherapy with autologous virus-specific T cells to affect the course of feline immunodeficiency virus (FIV) infection was explored in a group of specific-pathogen-free cats infected with FIV a minimum of 10 months earlier. Popliteal lymph node cells were stimulated by cocultivation with UV-inactivated autologous fibroblasts infected with recombinant vaccinia viruses expressing either FIV gag or env gene products, followed by expansion in interleukin-2. One or two infusions of both Gag- and Env-stimulated cells resulted in a slow increase in FIV-specific gamma interferon-secreting T cells in the circulation of cats. In the same animals, viral set points fluctuated widely during the first 2 to 3 weeks after adoptive transfer and then returned to pretreatment levels. The preexisting viral quasispecies was also found to be modulated, whereas no novel viral variants were detected. Circulating CD4(+) counts underwent a dramatic decline early after treatment. CD4/CD8 ratios remained instead essentially unchanged and eventually improved in some animals. In contrast, a single infusion of Gag-stimulated cells alone produced no apparent modulations of infection. SN - 1071-412X UR - https://www.unboundmedicine.com/medline/citation/15939748/Adoptive_immunotherapy_of_feline_immunodeficiency_virus_with_autologous_ex_vivo_stimulated_lymphoid_cells_modulates_virus_and_T_cell_subsets_in_blood_ DB - PRIME DP - Unbound Medicine ER -