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The CD94/NKG2C killer lectin-like receptor constitutes an alternative activation pathway for a subset of CD8+ T cells.
Eur J Immunol. 2005 Jul; 35(7):2071-80.EJ

Abstract

The CD94/NKG2C killer lectin-like receptor (KLR) specific for HLA-E is coupled to the KARAP/DAP12 adapter in a subset of NK cells, triggering their effector functions. We have studied the distribution and function of this KLR in T lymphocytes. Like other NK cell receptors (NKR), CD94/NKG2C was predominantly expressed by a CD8(+) T cell subset, though TCRgammadelta(+) NKG2C(+) and rare CD4(+) NKG2C(+) cells were also detected in some individuals. Coculture with the 721.221 HLA class I-deficient lymphoma cell line transfected with HLA-E (.221-AEH) induced IL-2Ralpha expression in CD94/NKG2C+ NK cells and a minor subset of CD94/NKG2C(+) T cells, promoting their proliferation; moreover, a similar response was triggered upon selective engagement of CD94/NKG2C with a specific mAb. CD8(+) TCRalphabeta CD94/NKG2C(+) T cell clones, that displayed different combinations of KIR and CD85j receptors, expressed KARAP/DAP12 which was co-precipitated by an anti-CD94 mAb. Specific engagement of the KLR triggered cytotoxicity and cytokine production in CD94/NKG2C(+) T cell clones, inducing as well IL-2Ralpha expression and a proliferative response. Altogether these results support that CD94/NKG2C may constitute an alternative T cell activation pathway capable of driving the expansion and triggering the effector functions of a CTL subset.

Authors+Show Affiliations

Molecular Immunopathology Unit, Universitat Pompeu Fabra (DCEXS), Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15940674

Citation

Gumá, Mónica, et al. "The CD94/NKG2C Killer Lectin-like Receptor Constitutes an Alternative Activation Pathway for a Subset of CD8+ T Cells." European Journal of Immunology, vol. 35, no. 7, 2005, pp. 2071-80.
Gumá M, Busch LK, Salazar-Fontana LI, et al. The CD94/NKG2C killer lectin-like receptor constitutes an alternative activation pathway for a subset of CD8+ T cells. Eur J Immunol. 2005;35(7):2071-80.
Gumá, M., Busch, L. K., Salazar-Fontana, L. I., Bellosillo, B., Morte, C., García, P., & López-Botet, M. (2005). The CD94/NKG2C killer lectin-like receptor constitutes an alternative activation pathway for a subset of CD8+ T cells. European Journal of Immunology, 35(7), 2071-80.
Gumá M, et al. The CD94/NKG2C Killer Lectin-like Receptor Constitutes an Alternative Activation Pathway for a Subset of CD8+ T Cells. Eur J Immunol. 2005;35(7):2071-80. PubMed PMID: 15940674.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The CD94/NKG2C killer lectin-like receptor constitutes an alternative activation pathway for a subset of CD8+ T cells. AU - Gumá,Mónica, AU - Busch,Lisa K, AU - Salazar-Fontana,Laura I, AU - Bellosillo,Beatriz, AU - Morte,Carles, AU - García,Pilar, AU - López-Botet,Miguel, PY - 2005/6/9/pubmed PY - 2005/9/7/medline PY - 2005/6/9/entrez SP - 2071 EP - 80 JF - European journal of immunology JO - Eur J Immunol VL - 35 IS - 7 N2 - The CD94/NKG2C killer lectin-like receptor (KLR) specific for HLA-E is coupled to the KARAP/DAP12 adapter in a subset of NK cells, triggering their effector functions. We have studied the distribution and function of this KLR in T lymphocytes. Like other NK cell receptors (NKR), CD94/NKG2C was predominantly expressed by a CD8(+) T cell subset, though TCRgammadelta(+) NKG2C(+) and rare CD4(+) NKG2C(+) cells were also detected in some individuals. Coculture with the 721.221 HLA class I-deficient lymphoma cell line transfected with HLA-E (.221-AEH) induced IL-2Ralpha expression in CD94/NKG2C+ NK cells and a minor subset of CD94/NKG2C(+) T cells, promoting their proliferation; moreover, a similar response was triggered upon selective engagement of CD94/NKG2C with a specific mAb. CD8(+) TCRalphabeta CD94/NKG2C(+) T cell clones, that displayed different combinations of KIR and CD85j receptors, expressed KARAP/DAP12 which was co-precipitated by an anti-CD94 mAb. Specific engagement of the KLR triggered cytotoxicity and cytokine production in CD94/NKG2C(+) T cell clones, inducing as well IL-2Ralpha expression and a proliferative response. Altogether these results support that CD94/NKG2C may constitute an alternative T cell activation pathway capable of driving the expansion and triggering the effector functions of a CTL subset. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/15940674/The_CD94/NKG2C_killer_lectin_like_receptor_constitutes_an_alternative_activation_pathway_for_a_subset_of_CD8+_T_cells_ L2 - https://doi.org/10.1002/eji.200425843 DB - PRIME DP - Unbound Medicine ER -