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[Effect of multiple micronutrients supplementation on anti-oxidative activity and oxidized DNA damage of lymphocytes in children].

Abstract

OBJECTIVE

To examine the effect of multiple micronutrients supplementation on anti-oxidative activity and decreasing oxidized DNA damage of lymphocytes in Chinese children.

METHODS

82 healthy children in a rural areas, aged 9-11 years, were selected and randomized allocated into group receiving supplements and control group with each of them 41. 24-hour dietary recall was used to collect data on daily nutrient intakes of the research subjects. The subjects in the supplement group were given vitamin A (VA) 600 microg, beta-carotene (beta-C) 1.0 mg, vitamin E (VE) 100 mg, vitamin C (VC) 300 mg and Na2SeO3(Se) 200 microg in a tablet on daily base while those in the control group took a same-sized color placebo tablet. The trial lasted 8 weeks. 5 ml blood samples from each subject were taken during 7 to 9 o'clock in the morning. DNA damage of lymphocytes and levels of plasma VA, VE, VC, beta-C, Se, malondialdehyde (MDA), activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were then analyzed twice before and after the 8-week of trial.

RESULTS

The low intakes of VA, VC and Se only accounted for 50.6%, 65.6% and 67.3% of their recommended nutrient intake (RNI) respectively. After the trial, levels of plasma beta-C, VA, VE, VC and Se in the supplemented group increased by 13.4%, 32.8%, 11.5%, 46.9% and 24.6% respectively, compared with the control group, indicating that nutritional status regarding antioxidant nutrients had largely been improved. GSH-Px activity had a significant increase in the supplement group than before the supplement and in the control group (P < 0.01). GSH-Px before the trial (the 100.4 U/ml) also showed significant increase after the trial (161.7 U/ml) (P < 0.01). However, the values of SOD and MDA significantly decreased after the trial. Analysis of DNA damage indicated that there was no significant difference in the intrinsic damage of DNA (P > 0.05). Significant decreases of oxidized DNA damage induced by 5 micromol/L, 10 micromol/L and 25 micromol/L H2O2 were found more in peripheral lymphocytes of the supplemented group, than in pre-supplement and the control group after the trial (P < 0.01).

CONCLUSION

Supplementation of multiple micronutrients could effectively increase the levels of beta-C, VA, VE, VC and Se in plasma, and GSH-Px activity. In the meantime, MDA and oxidized DNA damage induced by a low level H2O2 decreased significantly after the trial. The reason accounted for the decrease of SOD activity after the trial needs to be further studied.

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  • Authors+Show Affiliations

    ,

    Nutrition Department, Weifang People Hospital, Weifang 261041, China.

    , , ,

    Source

    MeSH

    Antioxidants
    Ascorbic Acid
    Child
    China
    DNA Damage
    Dietary Supplements
    Female
    Humans
    Lymphocytes
    Male
    Malondialdehyde
    Nutrition Surveys
    Oxidative Stress
    Rural Health
    Selenium
    Superoxide Dismutase
    Vitamin A
    Vitamin E
    Vitamins

    Pub Type(s)

    English Abstract
    Journal Article
    Randomized Controlled Trial
    Research Support, Non-U.S. Gov't

    Language

    chi

    PubMed ID

    15941534

    Citation

    Zhang, Ming, et al. "[Effect of Multiple Micronutrients Supplementation On Anti-oxidative Activity and Oxidized DNA Damage of Lymphocytes in Children]." Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, vol. 26, no. 4, 2005, pp. 268-72.
    Zhang M, Ma AG, Zhang XZ, et al. [Effect of multiple micronutrients supplementation on anti-oxidative activity and oxidized DNA damage of lymphocytes in children]. Zhonghua Liu Xing Bing Xue Za Zhi. 2005;26(4):268-72.
    Zhang, M., Ma, A. G., Zhang, X. Z., Ge, S., & Shi, X. X. (2005). [Effect of multiple micronutrients supplementation on anti-oxidative activity and oxidized DNA damage of lymphocytes in children]. Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 26(4), pp. 268-72.
    Zhang M, et al. [Effect of Multiple Micronutrients Supplementation On Anti-oxidative Activity and Oxidized DNA Damage of Lymphocytes in Children]. Zhonghua Liu Xing Bing Xue Za Zhi. 2005;26(4):268-72. PubMed PMID: 15941534.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - [Effect of multiple micronutrients supplementation on anti-oxidative activity and oxidized DNA damage of lymphocytes in children]. AU - Zhang,Ming, AU - Ma,Ai-guo, AU - Zhang,Xiu-zhen, AU - Ge,Sheng, AU - Shi,Xue-xiang, PY - 2005/6/9/pubmed PY - 2007/7/11/medline PY - 2005/6/9/entrez SP - 268 EP - 72 JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi JO - Zhonghua Liu Xing Bing Xue Za Zhi VL - 26 IS - 4 N2 - OBJECTIVE: To examine the effect of multiple micronutrients supplementation on anti-oxidative activity and decreasing oxidized DNA damage of lymphocytes in Chinese children. METHODS: 82 healthy children in a rural areas, aged 9-11 years, were selected and randomized allocated into group receiving supplements and control group with each of them 41. 24-hour dietary recall was used to collect data on daily nutrient intakes of the research subjects. The subjects in the supplement group were given vitamin A (VA) 600 microg, beta-carotene (beta-C) 1.0 mg, vitamin E (VE) 100 mg, vitamin C (VC) 300 mg and Na2SeO3(Se) 200 microg in a tablet on daily base while those in the control group took a same-sized color placebo tablet. The trial lasted 8 weeks. 5 ml blood samples from each subject were taken during 7 to 9 o'clock in the morning. DNA damage of lymphocytes and levels of plasma VA, VE, VC, beta-C, Se, malondialdehyde (MDA), activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were then analyzed twice before and after the 8-week of trial. RESULTS: The low intakes of VA, VC and Se only accounted for 50.6%, 65.6% and 67.3% of their recommended nutrient intake (RNI) respectively. After the trial, levels of plasma beta-C, VA, VE, VC and Se in the supplemented group increased by 13.4%, 32.8%, 11.5%, 46.9% and 24.6% respectively, compared with the control group, indicating that nutritional status regarding antioxidant nutrients had largely been improved. GSH-Px activity had a significant increase in the supplement group than before the supplement and in the control group (P < 0.01). GSH-Px before the trial (the 100.4 U/ml) also showed significant increase after the trial (161.7 U/ml) (P < 0.01). However, the values of SOD and MDA significantly decreased after the trial. Analysis of DNA damage indicated that there was no significant difference in the intrinsic damage of DNA (P > 0.05). Significant decreases of oxidized DNA damage induced by 5 micromol/L, 10 micromol/L and 25 micromol/L H2O2 were found more in peripheral lymphocytes of the supplemented group, than in pre-supplement and the control group after the trial (P < 0.01). CONCLUSION: Supplementation of multiple micronutrients could effectively increase the levels of beta-C, VA, VE, VC and Se in plasma, and GSH-Px activity. In the meantime, MDA and oxidized DNA damage induced by a low level H2O2 decreased significantly after the trial. The reason accounted for the decrease of SOD activity after the trial needs to be further studied. SN - 0254-6450 UR - https://www.unboundmedicine.com/medline/citation/15941534/[Effect_of_multiple_micronutrients_supplementation_on_anti_oxidative_activity_and_oxidized_DNA_damage_of_lymphocytes_in_children]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0254-6450&amp;year=2005&amp;vol=26&amp;issue=4&amp;fpage=268 DB - PRIME DP - Unbound Medicine ER -