Tags

Type your tag names separated by a space and hit enter

Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens in endodontic lesions detected by culture and by PCR.
Oral Microbiol Immunol. 2005 Aug; 20(4):211-5.OM

Abstract

he aim of this study was to investigate the presence of four black-pigmented bacteria, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens, in endodontic infections by culture and polymerase chain reaction (PCR) analyses. Microbial samples were obtained from 50 teeth with untreated necrotic pulps (primary infection) and from 50 teeth with failing endodontic treatment (secondary infection). Microbiological strict anaerobic techniques were used for serial dilution, plating, incubation, and identification. For PCR detection, the samples were analyzed using species-specific primers of 16S rDNA and the downstream intergenic spacer region. Culture and PCR detected the test species in 13/100 and 50/100 of the study teeth, respectively. The organisms were cultured from 11/50 (22%) of primarily infected root canal samples and from 2/50 (4%) of secondary root canal samples. PCR detection identified the target species in 32/50 (64%) and 18/50 (36%) of primary and secondary infections, respectively. P. gingivalis was rarely isolated by culture methods (1%), but was the most frequently identified test species by PCR (38%). Similarly, P. endodontalis was not recovered by culture from any tooth studied, but was detected by PCR in 25% of the sampled teeth. PCR-based identification also showed higher detection rates of P. intermedia (33%) and P. nigrescens (22%) than culture (13%). In conclusion, P. gingivalis, P. endodontalis, P. intermedia, and P. nigrescens were identified more frequently in teeth with necrotic pulp than in teeth with failing endodontic treatment. Also, a higher frequency of black-pigmented species was detected by PCR than by culture.

Authors+Show Affiliations

Endodontic Area, Dental School of Piracicaba, State University of Campinas-UNICAMP, Piracicaba, SP, Brazil. bpgomes@fop.unicamp.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15943764

Citation

Gomes, B P F A., et al. "Porphyromonas Gingivalis, Porphyromonas Endodontalis, Prevotella Intermedia and Prevotella Nigrescens in Endodontic Lesions Detected By Culture and By PCR." Oral Microbiology and Immunology, vol. 20, no. 4, 2005, pp. 211-5.
Gomes BP, Jacinto RC, Pinheiro ET, et al. Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens in endodontic lesions detected by culture and by PCR. Oral Microbiol Immunol. 2005;20(4):211-5.
Gomes, B. P., Jacinto, R. C., Pinheiro, E. T., Sousa, E. L., Zaia, A. A., Ferraz, C. C., & Souza-Filho, F. J. (2005). Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens in endodontic lesions detected by culture and by PCR. Oral Microbiology and Immunology, 20(4), 211-5.
Gomes BP, et al. Porphyromonas Gingivalis, Porphyromonas Endodontalis, Prevotella Intermedia and Prevotella Nigrescens in Endodontic Lesions Detected By Culture and By PCR. Oral Microbiol Immunol. 2005;20(4):211-5. PubMed PMID: 15943764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens in endodontic lesions detected by culture and by PCR. AU - Gomes,B P F A, AU - Jacinto,R C, AU - Pinheiro,E T, AU - Sousa,E L R, AU - Zaia,A A, AU - Ferraz,C C R, AU - Souza-Filho,F J, PY - 2005/6/10/pubmed PY - 2005/7/27/medline PY - 2005/6/10/entrez SP - 211 EP - 5 JF - Oral microbiology and immunology JO - Oral Microbiol. Immunol. VL - 20 IS - 4 N2 - he aim of this study was to investigate the presence of four black-pigmented bacteria, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens, in endodontic infections by culture and polymerase chain reaction (PCR) analyses. Microbial samples were obtained from 50 teeth with untreated necrotic pulps (primary infection) and from 50 teeth with failing endodontic treatment (secondary infection). Microbiological strict anaerobic techniques were used for serial dilution, plating, incubation, and identification. For PCR detection, the samples were analyzed using species-specific primers of 16S rDNA and the downstream intergenic spacer region. Culture and PCR detected the test species in 13/100 and 50/100 of the study teeth, respectively. The organisms were cultured from 11/50 (22%) of primarily infected root canal samples and from 2/50 (4%) of secondary root canal samples. PCR detection identified the target species in 32/50 (64%) and 18/50 (36%) of primary and secondary infections, respectively. P. gingivalis was rarely isolated by culture methods (1%), but was the most frequently identified test species by PCR (38%). Similarly, P. endodontalis was not recovered by culture from any tooth studied, but was detected by PCR in 25% of the sampled teeth. PCR-based identification also showed higher detection rates of P. intermedia (33%) and P. nigrescens (22%) than culture (13%). In conclusion, P. gingivalis, P. endodontalis, P. intermedia, and P. nigrescens were identified more frequently in teeth with necrotic pulp than in teeth with failing endodontic treatment. Also, a higher frequency of black-pigmented species was detected by PCR than by culture. SN - 0902-0055 UR - https://www.unboundmedicine.com/medline/citation/15943764/Porphyromonas_gingivalis_Porphyromonas_endodontalis_Prevotella_intermedia_and_Prevotella_nigrescens_in_endodontic_lesions_detected_by_culture_and_by_PCR_ L2 - https://doi.org/10.1111/j.1399-302X.2005.00214.x DB - PRIME DP - Unbound Medicine ER -