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Drugs in oral fluid Part I. Validation of an analytical procedure for licit and illicit drugs in oral fluid.
Forensic Sci Int. 2005 Jun 10; 150(2-3):191-8.FS

Abstract

A qualitative and quantitative analytical method was developed and validated for the determination of 49 licit and illicit drugs in oral fluid. Small oral fluid samples, volume 1mL, were collected from volunteers using a modified Omni-Sal device and the analytes were extracted from an oral fluid/buffer mixture using a single Bond Elut Certify solid phase extraction cartridge. Liquid chromatography-tandem mass spectrometry (LC-MS-MS) and gas chromatography-repetitive full scan mass spectrometry (GC-MS) were used in parallel to analyze the extracts for the targeted drugs. Extracts were analyzed by GC-MS in their underivatized form and as their pentafluoropropionyl derivatives. Deuterated internal standards were used for quantification of drugs of abuse by LC-MS-MS to minimize matrix effects. Methadone-d(9) and tumoxetine were used as the internal standards for quantification of non-derivatized and derivatized analytes respectively by GC-MS. Linearity was demonstrated over the range 5-200 ng/mL and limits of detection were less than 4 ng/mL for each drug analyzed. The method demonstrated acceptable recoveries for most of the analytes and good intra- and inter-day precision. Acquisition of data by repetitive full scan GC-MS allows the addition of further analytes to the target menu.

Authors+Show Affiliations

Department of Forensic Medicine and Science, University of Glasgow, Glasgow, Scotland G12 8QQ, UK. f.wylie@formed.gla.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

15944059

Citation

Wylie, F M., et al. "Drugs in Oral Fluid Part I. Validation of an Analytical Procedure for Licit and Illicit Drugs in Oral Fluid." Forensic Science International, vol. 150, no. 2-3, 2005, pp. 191-8.
Wylie FM, Torrance H, Anderson RA, et al. Drugs in oral fluid Part I. Validation of an analytical procedure for licit and illicit drugs in oral fluid. Forensic Sci Int. 2005;150(2-3):191-8.
Wylie, F. M., Torrance, H., Anderson, R. A., & Oliver, J. S. (2005). Drugs in oral fluid Part I. Validation of an analytical procedure for licit and illicit drugs in oral fluid. Forensic Science International, 150(2-3), 191-8.
Wylie FM, et al. Drugs in Oral Fluid Part I. Validation of an Analytical Procedure for Licit and Illicit Drugs in Oral Fluid. Forensic Sci Int. 2005 Jun 10;150(2-3):191-8. PubMed PMID: 15944059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Drugs in oral fluid Part I. Validation of an analytical procedure for licit and illicit drugs in oral fluid. AU - Wylie,F M, AU - Torrance,H, AU - Anderson,R A, AU - Oliver,J S, Y1 - 2005/04/14/ PY - 2004/11/13/received PY - 2005/02/12/revised PY - 2005/02/12/accepted PY - 2005/6/10/pubmed PY - 2005/9/9/medline PY - 2005/6/10/entrez SP - 191 EP - 8 JF - Forensic science international JO - Forensic Sci Int VL - 150 IS - 2-3 N2 - A qualitative and quantitative analytical method was developed and validated for the determination of 49 licit and illicit drugs in oral fluid. Small oral fluid samples, volume 1mL, were collected from volunteers using a modified Omni-Sal device and the analytes were extracted from an oral fluid/buffer mixture using a single Bond Elut Certify solid phase extraction cartridge. Liquid chromatography-tandem mass spectrometry (LC-MS-MS) and gas chromatography-repetitive full scan mass spectrometry (GC-MS) were used in parallel to analyze the extracts for the targeted drugs. Extracts were analyzed by GC-MS in their underivatized form and as their pentafluoropropionyl derivatives. Deuterated internal standards were used for quantification of drugs of abuse by LC-MS-MS to minimize matrix effects. Methadone-d(9) and tumoxetine were used as the internal standards for quantification of non-derivatized and derivatized analytes respectively by GC-MS. Linearity was demonstrated over the range 5-200 ng/mL and limits of detection were less than 4 ng/mL for each drug analyzed. The method demonstrated acceptable recoveries for most of the analytes and good intra- and inter-day precision. Acquisition of data by repetitive full scan GC-MS allows the addition of further analytes to the target menu. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/15944059/Drugs_in_oral_fluid_Part_I__Validation_of_an_analytical_procedure_for_licit_and_illicit_drugs_in_oral_fluid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(05)00126-X DB - PRIME DP - Unbound Medicine ER -