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Analysis of Delta9-tetrahydrocannabinol in oral fluid samples using solid-phase extraction and high-performance liquid chromatography-electrospray ionization mass spectrometry.
Forensic Sci Int. 2005 Jun 10; 150(2-3):205-11.FS

Abstract

An analytical method using solid-phase extraction (SPE) and high-performance liquid chromatography-mass spectrometry (LC-MS) has been developed and validated for the confirmation of Delta(9)-tetrahydrocannabinol (THC) in oral fluid samples. Oral fluid was extracted using Bond Elut LRC-Certify solid-phase extraction columns (10 cm(3), 300 mg) and elution performed with n-hexane/ethyl acetate. Quantitation made use of the selected ion-recording mode (SIR) using the most abundant characteristic ion [THC+H(+)], m/z 315.31 and the fragment ion, m/z 193.13 for confirmation, and m/z 318.00 for the protonated internal standard, [d(3)-THC+H(+)]. The method proved to be precise for THC, in terms of both intra-day and inter-day analyses, with coefficients of variation less than 10%, and the calculated extraction efficiencies for THC ranged from 76 to 83%. Calibration standards spiked with THC between 2 and 100 ng/mL showed a linear relationship (r(2)=0.999). The method presented was applied to the oral fluid samples taken from the volunteers during the largest music event in Portugal, named Rock in Rio-Lisboa. Oral fluid was collected from 40 persons by expectoration and with Salivette. In 55% of the samples obtained by expectorating, THC was detected with concentration ranges from 1033 to 6552 ng/mL and in 45% of cases THC was detected at concentrations between 51 and 937 ng/mL. However, using Salivette collection, 26 of the 40 cases had an undetectable THC.

Authors+Show Affiliations

Delegation of Coimbra, National Institute of Legal Medicine, Largo da Sé Nova, 3000-213 Coimbra, Portugal. helena.teixeira@dcinml.mj.ptNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15944061

Citation

Teixeira, Helena, et al. "Analysis of Delta9-tetrahydrocannabinol in Oral Fluid Samples Using Solid-phase Extraction and High-performance Liquid Chromatography-electrospray Ionization Mass Spectrometry." Forensic Science International, vol. 150, no. 2-3, 2005, pp. 205-11.
Teixeira H, Proença P, Verstraete A, et al. Analysis of Delta9-tetrahydrocannabinol in oral fluid samples using solid-phase extraction and high-performance liquid chromatography-electrospray ionization mass spectrometry. Forensic Sci Int. 2005;150(2-3):205-11.
Teixeira, H., Proença, P., Verstraete, A., Corte-Real, F., & Vieira, D. N. (2005). Analysis of Delta9-tetrahydrocannabinol in oral fluid samples using solid-phase extraction and high-performance liquid chromatography-electrospray ionization mass spectrometry. Forensic Science International, 150(2-3), 205-11.
Teixeira H, et al. Analysis of Delta9-tetrahydrocannabinol in Oral Fluid Samples Using Solid-phase Extraction and High-performance Liquid Chromatography-electrospray Ionization Mass Spectrometry. Forensic Sci Int. 2005 Jun 10;150(2-3):205-11. PubMed PMID: 15944061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of Delta9-tetrahydrocannabinol in oral fluid samples using solid-phase extraction and high-performance liquid chromatography-electrospray ionization mass spectrometry. AU - Teixeira,Helena, AU - Proença,Paula, AU - Verstraete,Alain, AU - Corte-Real,Francisco, AU - Vieira,Duarte Nuno, Y1 - 2005/04/15/ PY - 2004/09/21/received PY - 2004/11/10/revised PY - 2004/11/10/accepted PY - 2005/6/10/pubmed PY - 2005/9/9/medline PY - 2005/6/10/entrez SP - 205 EP - 11 JF - Forensic science international JO - Forensic Sci Int VL - 150 IS - 2-3 N2 - An analytical method using solid-phase extraction (SPE) and high-performance liquid chromatography-mass spectrometry (LC-MS) has been developed and validated for the confirmation of Delta(9)-tetrahydrocannabinol (THC) in oral fluid samples. Oral fluid was extracted using Bond Elut LRC-Certify solid-phase extraction columns (10 cm(3), 300 mg) and elution performed with n-hexane/ethyl acetate. Quantitation made use of the selected ion-recording mode (SIR) using the most abundant characteristic ion [THC+H(+)], m/z 315.31 and the fragment ion, m/z 193.13 for confirmation, and m/z 318.00 for the protonated internal standard, [d(3)-THC+H(+)]. The method proved to be precise for THC, in terms of both intra-day and inter-day analyses, with coefficients of variation less than 10%, and the calculated extraction efficiencies for THC ranged from 76 to 83%. Calibration standards spiked with THC between 2 and 100 ng/mL showed a linear relationship (r(2)=0.999). The method presented was applied to the oral fluid samples taken from the volunteers during the largest music event in Portugal, named Rock in Rio-Lisboa. Oral fluid was collected from 40 persons by expectoration and with Salivette. In 55% of the samples obtained by expectorating, THC was detected with concentration ranges from 1033 to 6552 ng/mL and in 45% of cases THC was detected at concentrations between 51 and 937 ng/mL. However, using Salivette collection, 26 of the 40 cases had an undetectable THC. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/15944061/Analysis_of_Delta9_tetrahydrocannabinol_in_oral_fluid_samples_using_solid_phase_extraction_and_high_performance_liquid_chromatography_electrospray_ionization_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(05)00128-3 DB - PRIME DP - Unbound Medicine ER -