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Determination of MDMA, MDA, MDEA and MBDB in oral fluid using high performance liquid chromatography with native fluorescence detection.
Forensic Sci Int. 2005 Jun 10; 150(2-3):221-6.FS

Abstract

This paper describes the analytical methodology for the determination of MDMA, MDA, MDEA and MBDB in oral fluid. After a liquid-liquid extraction, the analysis was carried out by high performance liquid chromatography (HPLC), with fluorescence detection. The detector wavelength was fixed at 285 nm for excitation and 320 nm for emission. The mobile phase, a mixture of phosphate buffer (pH=5) and acetonitrile (75:25), and the column, Kromasil 100 C8 5 microm 250 mm x 4.6mm, allowed good separation of the compounds in an isocratic mode in only 10 min. The method was validated and showed good limits of detection (2 ng/mL) and quantitation (10 ng/mL) for all the amphetamine derivatives. No interfering substances were detected. A stability study of these compounds in oral fluid stored at three different temperatures (-18, 4 and 20 degrees C) over 10 weeks was conducted, showing a time-dependent degradation of the four compounds.

Authors+Show Affiliations

Forensic Toxicology Service, Institute of Legal Medicine, University of Santiago de Compostela, c/ San Francisco s/n, 15782 Santiago de Compostela, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15944063

Citation

Concheiro, Marta, et al. "Determination of MDMA, MDA, MDEA and MBDB in Oral Fluid Using High Performance Liquid Chromatography With Native Fluorescence Detection." Forensic Science International, vol. 150, no. 2-3, 2005, pp. 221-6.
Concheiro M, de Castro A, Quintela O, et al. Determination of MDMA, MDA, MDEA and MBDB in oral fluid using high performance liquid chromatography with native fluorescence detection. Forensic Sci Int. 2005;150(2-3):221-6.
Concheiro, M., de Castro, A., Quintela, O., López-Rivadulla, M., & Cruz, A. (2005). Determination of MDMA, MDA, MDEA and MBDB in oral fluid using high performance liquid chromatography with native fluorescence detection. Forensic Science International, 150(2-3), 221-6.
Concheiro M, et al. Determination of MDMA, MDA, MDEA and MBDB in Oral Fluid Using High Performance Liquid Chromatography With Native Fluorescence Detection. Forensic Sci Int. 2005 Jun 10;150(2-3):221-6. PubMed PMID: 15944063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of MDMA, MDA, MDEA and MBDB in oral fluid using high performance liquid chromatography with native fluorescence detection. AU - Concheiro,Marta, AU - de Castro,Ana, AU - Quintela,Oscar, AU - López-Rivadulla,Manuel, AU - Cruz,Angelines, PY - 2004/11/24/received PY - 2004/12/02/revised PY - 2004/12/02/accepted PY - 2005/6/10/pubmed PY - 2005/9/9/medline PY - 2005/6/10/entrez SP - 221 EP - 6 JF - Forensic science international JO - Forensic Sci Int VL - 150 IS - 2-3 N2 - This paper describes the analytical methodology for the determination of MDMA, MDA, MDEA and MBDB in oral fluid. After a liquid-liquid extraction, the analysis was carried out by high performance liquid chromatography (HPLC), with fluorescence detection. The detector wavelength was fixed at 285 nm for excitation and 320 nm for emission. The mobile phase, a mixture of phosphate buffer (pH=5) and acetonitrile (75:25), and the column, Kromasil 100 C8 5 microm 250 mm x 4.6mm, allowed good separation of the compounds in an isocratic mode in only 10 min. The method was validated and showed good limits of detection (2 ng/mL) and quantitation (10 ng/mL) for all the amphetamine derivatives. No interfering substances were detected. A stability study of these compounds in oral fluid stored at three different temperatures (-18, 4 and 20 degrees C) over 10 weeks was conducted, showing a time-dependent degradation of the four compounds. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/15944063/Determination_of_MDMA_MDA_MDEA_and_MBDB_in_oral_fluid_using_high_performance_liquid_chromatography_with_native_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(05)00130-1 DB - PRIME DP - Unbound Medicine ER -