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Anaerobic induction of isocitrate lyase and malate synthase in submerged rice seedlings indicates the important metabolic role of the glyoxylate cycle.
Acta Biochim Biophys Sin (Shanghai). 2005 Jun; 37(6):406-14.AB

Abstract

The glyoxylate cycle is a modified form of the tricarboxylic acid cycle that converts C2 compounds into C4 dicarboxylic acids at plant developmental stages. By studying submerged rice seedlings, we revealed the activation of the glyoxylate cycle by identifying the increased transcripts of mRNAs of the genes of isocitrate lyase (ICL) and malate synthase (MS), two characteristic enzymes of the glyoxylate cycle. Northern blot analysis showed that ICL and MS were activated in the prolonged anaerobic environment. The activity assay of pyruvate decarboxylase and ICL in the submerged seedlings indicated an 8.8-fold and 3.5-fold increase over that in the unsubmerged seedlings, respectively. The activity assay of acetyl-coenzyme A synthetase in the submerged seedlings indicated a 3-fold increase over that in the unsubmerged seedlings, which is important for initiating acetate metabolism. Consequently, we concluded that the glyoxylate cycle was involved in acetate metabolism under anaerobic conditions.

Authors+Show Affiliations

Lu Y
National Center for Gene Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China.
Wu YR
No affiliation info available
Han B
No affiliation info available

MeSH

Acetate-CoA LigaseAmino Acid SequenceAnaerobiosisCitric Acid CycleCloning, MolecularEnzyme InductionGlyoxylatesIsocitrate LyaseMalate SynthaseMolecular Sequence DataOryzaSeedlingsSequence Alignment

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15944756

Citation

Lu, Ying, et al. "Anaerobic Induction of Isocitrate Lyase and Malate Synthase in Submerged Rice Seedlings Indicates the Important Metabolic Role of the Glyoxylate Cycle." Acta Biochimica Et Biophysica Sinica, vol. 37, no. 6, 2005, pp. 406-14.
Lu Y, Wu YR, Han B. Anaerobic induction of isocitrate lyase and malate synthase in submerged rice seedlings indicates the important metabolic role of the glyoxylate cycle. Acta Biochim Biophys Sin (Shanghai). 2005;37(6):406-14.
Lu, Y., Wu, Y. R., & Han, B. (2005). Anaerobic induction of isocitrate lyase and malate synthase in submerged rice seedlings indicates the important metabolic role of the glyoxylate cycle. Acta Biochimica Et Biophysica Sinica, 37(6), 406-14.
Lu Y, Wu YR, Han B. Anaerobic Induction of Isocitrate Lyase and Malate Synthase in Submerged Rice Seedlings Indicates the Important Metabolic Role of the Glyoxylate Cycle. Acta Biochim Biophys Sin (Shanghai). 2005;37(6):406-14. PubMed PMID: 15944756.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anaerobic induction of isocitrate lyase and malate synthase in submerged rice seedlings indicates the important metabolic role of the glyoxylate cycle. AU - Lu,Ying, AU - Wu,Yong-Rui, AU - Han,Bin, PY - 2005/6/10/pubmed PY - 2005/10/12/medline PY - 2005/6/10/entrez SP - 406 EP - 14 JF - Acta biochimica et biophysica Sinica JO - Acta Biochim Biophys Sin (Shanghai) VL - 37 IS - 6 N2 - The glyoxylate cycle is a modified form of the tricarboxylic acid cycle that converts C2 compounds into C4 dicarboxylic acids at plant developmental stages. By studying submerged rice seedlings, we revealed the activation of the glyoxylate cycle by identifying the increased transcripts of mRNAs of the genes of isocitrate lyase (ICL) and malate synthase (MS), two characteristic enzymes of the glyoxylate cycle. Northern blot analysis showed that ICL and MS were activated in the prolonged anaerobic environment. The activity assay of pyruvate decarboxylase and ICL in the submerged seedlings indicated an 8.8-fold and 3.5-fold increase over that in the unsubmerged seedlings, respectively. The activity assay of acetyl-coenzyme A synthetase in the submerged seedlings indicated a 3-fold increase over that in the unsubmerged seedlings, which is important for initiating acetate metabolism. Consequently, we concluded that the glyoxylate cycle was involved in acetate metabolism under anaerobic conditions. SN - 1672-9145 UR - https://www.unboundmedicine.com/medline/citation/15944756/Anaerobic_induction_of_isocitrate_lyase_and_malate_synthase_in_submerged_rice_seedlings_indicates_the_important_metabolic_role_of_the_glyoxylate_cycle_ DB - PRIME DP - Unbound Medicine ER -