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The phenotype of type 1 and type 2 CD8+ T cells activated in vitro is affected by culture conditions and correlates with effector activity.
Immunology. 2005 Jul; 115(3):315-24.I

Abstract

We used various culture conditions to generate type 1 (Tc1) or type 2 (Tc2) cytotoxic T cells in vitro. T-cell receptor (TCR) transgenic T cells were cultured with antigen and spleen cells, or antigen and dendritic cells (DC), or anti-CD3 and anti-CD28. Tc1 cultures contained interleukin (IL)-2 and IL-6, and Tc2 cultures contained IL-2, IL-6 and IL-4. Tc2 cells generated in each culture condition acquired a CD62L(low) CD44(high) phenotype, had high cytotoxic activity, and secreted IL-4, IL-5 and moderate amounts of interferon-gamma (IFN-gamma). In contrast, the phenotype and function of Tc1 cells varied depending on culture conditions. Tc1 cells from anti-CD3 and anti-CD28 cultures had high cytotoxic activity and were CD62L(low) CD44(high), while Tc1 cells from antigen and spleen cell cultures had low cytotoxic activity and were CD62L(high) CD44(low). Tc1 cells from antigen and DC cultures had an intermediate phenotype. All Tc1 cells secreted high amounts of IFN-gamma, but only Tc1 from anti-CD3 and anti-CD28 cultures had antitumour activity in vivo. Differences were not caused by suboptimal culture conditions, as Tc1 cells divided at a similar rate whether cultured with antigen and spleen cells or with anti-CD3 and anti-CD28. We conclude that IL-4 not only induces 'type 2' cytokine secretion in CD8(+) T cells, but also affects their expression of surface markers and cytotoxic activity.

Authors+Show Affiliations

Malaghan Institute of Medical Research, Wellington, New Zealand.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15946249

Citation

Kemp, Roslyn A., et al. "The Phenotype of Type 1 and Type 2 CD8+ T Cells Activated in Vitro Is Affected By Culture Conditions and Correlates With Effector Activity." Immunology, vol. 115, no. 3, 2005, pp. 315-24.
Kemp RA, Bäckström BT, Ronchese F. The phenotype of type 1 and type 2 CD8+ T cells activated in vitro is affected by culture conditions and correlates with effector activity. Immunology. 2005;115(3):315-24.
Kemp, R. A., Bäckström, B. T., & Ronchese, F. (2005). The phenotype of type 1 and type 2 CD8+ T cells activated in vitro is affected by culture conditions and correlates with effector activity. Immunology, 115(3), 315-24.
Kemp RA, Bäckström BT, Ronchese F. The Phenotype of Type 1 and Type 2 CD8+ T Cells Activated in Vitro Is Affected By Culture Conditions and Correlates With Effector Activity. Immunology. 2005;115(3):315-24. PubMed PMID: 15946249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The phenotype of type 1 and type 2 CD8+ T cells activated in vitro is affected by culture conditions and correlates with effector activity. AU - Kemp,Roslyn A, AU - Bäckström,B Thomas, AU - Ronchese,Franca, PY - 2005/6/11/pubmed PY - 2005/7/30/medline PY - 2005/6/11/entrez SP - 315 EP - 24 JF - Immunology JO - Immunology VL - 115 IS - 3 N2 - We used various culture conditions to generate type 1 (Tc1) or type 2 (Tc2) cytotoxic T cells in vitro. T-cell receptor (TCR) transgenic T cells were cultured with antigen and spleen cells, or antigen and dendritic cells (DC), or anti-CD3 and anti-CD28. Tc1 cultures contained interleukin (IL)-2 and IL-6, and Tc2 cultures contained IL-2, IL-6 and IL-4. Tc2 cells generated in each culture condition acquired a CD62L(low) CD44(high) phenotype, had high cytotoxic activity, and secreted IL-4, IL-5 and moderate amounts of interferon-gamma (IFN-gamma). In contrast, the phenotype and function of Tc1 cells varied depending on culture conditions. Tc1 cells from anti-CD3 and anti-CD28 cultures had high cytotoxic activity and were CD62L(low) CD44(high), while Tc1 cells from antigen and spleen cell cultures had low cytotoxic activity and were CD62L(high) CD44(low). Tc1 cells from antigen and DC cultures had an intermediate phenotype. All Tc1 cells secreted high amounts of IFN-gamma, but only Tc1 from anti-CD3 and anti-CD28 cultures had antitumour activity in vivo. Differences were not caused by suboptimal culture conditions, as Tc1 cells divided at a similar rate whether cultured with antigen and spleen cells or with anti-CD3 and anti-CD28. We conclude that IL-4 not only induces 'type 2' cytokine secretion in CD8(+) T cells, but also affects their expression of surface markers and cytotoxic activity. SN - 0019-2805 UR - https://www.unboundmedicine.com/medline/citation/15946249/The_phenotype_of_type_1_and_type_2_CD8+_T_cells_activated_in_vitro_is_affected_by_culture_conditions_and_correlates_with_effector_activity_ L2 - https://doi.org/10.1111/j.1365-2567.2005.02168.x DB - PRIME DP - Unbound Medicine ER -