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Identification of flavonoids and their glycosides by high-performance liquid chromatography with electrospray ionization mass spectrometry and with diode array ultraviolet detection.
Eur J Mass Spectrom (Chichester). 2005; 11(1):93-101.EJ

Abstract

Identification of flavonoids and flavonoid glycosides was carried out on Psidium guajava Linn leaves by means of high-performance liquid chromatography ultraviolet (HPLC-UV) analysis and HPLC mass spectrometry. By using HPLC-UV, two known phenolics (gallic acid and quercetin) and five newly reported ones (procatechuic acid, chlorogenic acid, caffeic acid, kaempferol and ferulic acid) were identified in alcohol guava leaf extract. Structural information about the compounds was obtained from the retention times, the UV spectra and mass spectra without the need to isolate the individual compounds. Two flavonoids (quercetin and kaempferol) and four flavonoid glycosides (three known components, quercetin 3-O-alpha-L-arabinoside, quercetin 3-O-beta-D-glucoside and quercetin 3-O-beta-D-galactoside, along with one novel compound, kaempferol-glycoside) and three other unknown compounds have been identified in the fractions.

Authors+Show Affiliations

Department of Food Science and Technology,Southern Yangtze University, Wuxi 214036, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15947448

Citation

Liang, Qingrong, et al. "Identification of Flavonoids and Their Glycosides By High-performance Liquid Chromatography With Electrospray Ionization Mass Spectrometry and With Diode Array Ultraviolet Detection." European Journal of Mass Spectrometry (Chichester, England), vol. 11, no. 1, 2005, pp. 93-101.
Liang Q, Qian H, Yao W. Identification of flavonoids and their glycosides by high-performance liquid chromatography with electrospray ionization mass spectrometry and with diode array ultraviolet detection. Eur J Mass Spectrom (Chichester). 2005;11(1):93-101.
Liang, Q., Qian, H., & Yao, W. (2005). Identification of flavonoids and their glycosides by high-performance liquid chromatography with electrospray ionization mass spectrometry and with diode array ultraviolet detection. European Journal of Mass Spectrometry (Chichester, England), 11(1), 93-101.
Liang Q, Qian H, Yao W. Identification of Flavonoids and Their Glycosides By High-performance Liquid Chromatography With Electrospray Ionization Mass Spectrometry and With Diode Array Ultraviolet Detection. Eur J Mass Spectrom (Chichester). 2005;11(1):93-101. PubMed PMID: 15947448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of flavonoids and their glycosides by high-performance liquid chromatography with electrospray ionization mass spectrometry and with diode array ultraviolet detection. AU - Liang,Qingrong, AU - Qian,He, AU - Yao,Weirong, PY - 2005/6/11/pubmed PY - 2005/7/9/medline PY - 2005/6/11/entrez SP - 93 EP - 101 JF - European journal of mass spectrometry (Chichester, England) JO - Eur J Mass Spectrom (Chichester) VL - 11 IS - 1 N2 - Identification of flavonoids and flavonoid glycosides was carried out on Psidium guajava Linn leaves by means of high-performance liquid chromatography ultraviolet (HPLC-UV) analysis and HPLC mass spectrometry. By using HPLC-UV, two known phenolics (gallic acid and quercetin) and five newly reported ones (procatechuic acid, chlorogenic acid, caffeic acid, kaempferol and ferulic acid) were identified in alcohol guava leaf extract. Structural information about the compounds was obtained from the retention times, the UV spectra and mass spectra without the need to isolate the individual compounds. Two flavonoids (quercetin and kaempferol) and four flavonoid glycosides (three known components, quercetin 3-O-alpha-L-arabinoside, quercetin 3-O-beta-D-glucoside and quercetin 3-O-beta-D-galactoside, along with one novel compound, kaempferol-glycoside) and three other unknown compounds have been identified in the fractions. SN - 1469-0667 UR - https://www.unboundmedicine.com/medline/citation/15947448/Identification_of_flavonoids_and_their_glycosides_by_high_performance_liquid_chromatography_with_electrospray_ionization_mass_spectrometry_and_with_diode_array_ultraviolet_detection_ L2 - https://journals.sagepub.com/doi/10.1255/ejms.710?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -