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Identification and validation of QTL for Sclerotinia midstalk rot resistance in sunflower by selective genotyping.
Theor Appl Genet. 2005 Jul; 111(2):233-42.TA

Abstract

Midstalk rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is an important cause of yield loss in sunflower (Helianthus annuus L.). Objectives of this study were to: (1) estimate the number, genomic positions and genetic effects of quantitative trait loci (QTL) for resistance to midstalk rot in line TUB-5-3234, derived from an interspecific cross; (2) determine congruency of QTL between this line and other sources of resistance; and (3) make inferences about the efficiency of selective genotyping (SG) in detecting QTL conferring midstalk rot resistance in sunflower. Phenotypic data for three resistance (stem lesion, leaf lesion and speed of fungal growth) and two morphological (leaf length and leaf length with petiole) traits were obtained from 434 F3 families from cross CM625 (susceptible) x TUB-5-3234 (resistant) under artificial infection in field experiments across two environments. The SG was applied by choosing the 60 most resistant and the 60 most susceptible F3 families for stem lesion. For genotyping of the respective F2 plants, 78 simple sequence repeat markers were used. Genotypic variances were highly significant for all traits. Heritabilities and genotypic correlations between reMidstalk rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is an important cause of yield loss in sunflower (Helianthus annuus L.). Objectives of this study were to: (1) estimate the number, genomic positions and genetic effects of quantitative trait loci (QTL) for resistance to midstalk rot in line TUB-5-3234, derived from an interspecific cross; (2) determine congruency of QTL between this line and other sources of resistance; and (3) make inferences about the efficiency of selective genotyping (SG) in detecting QTL conferring midstalk rot resistance in sunflower. Phenotypic data for three resistance (stem lesion, leaf lesion and speed of fungal growth) and two morphological (leaf length and leaf length with petiole) traits were obtained from 434 F3 families from cross CM625 (susceptible) x TUB-5-3234 (resistant) under artificial infection in field experiments across two environments. The SG was applied by choosing the 60 most resistant and the 60 most susceptible F3 families for stem lesion. For genotyping of the respective F2 plants, 78 simple sequence repeat markers were used. Genotypic variances were highly significant for all traits. Heritabilities and genotypic correlations between resistance traits were moderate to high. Three to four putative QTL were detected for each resistance trait explaining between 40.8% and 72.7% of the genotypic variance (PTS). Two QTL for stem lesion showed large genetic effects and corroborated earlier findings from the cross NDBLOSsel (resistant) x CM625 (susceptible). Our results suggest that SG can be efficiently used for QTL detection and the analysis of congruency for resistance genes across populations.

Authors+Show Affiliations

State Plant Breeding Institute (720), University of Hohenheim, Stuttgart, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15947909

Citation

Micic, Z, et al. "Identification and Validation of QTL for Sclerotinia Midstalk Rot Resistance in Sunflower By Selective Genotyping." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 111, no. 2, 2005, pp. 233-42.
Micic Z, Hahn V, Bauer E, et al. Identification and validation of QTL for Sclerotinia midstalk rot resistance in sunflower by selective genotyping. Theor Appl Genet. 2005;111(2):233-42.
Micic, Z., Hahn, V., Bauer, E., Melchinger, A. E., Knapp, S. J., Tang, S., & Schön, C. C. (2005). Identification and validation of QTL for Sclerotinia midstalk rot resistance in sunflower by selective genotyping. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 111(2), 233-42.
Micic Z, et al. Identification and Validation of QTL for Sclerotinia Midstalk Rot Resistance in Sunflower By Selective Genotyping. Theor Appl Genet. 2005;111(2):233-42. PubMed PMID: 15947909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and validation of QTL for Sclerotinia midstalk rot resistance in sunflower by selective genotyping. AU - Micic,Z, AU - Hahn,V, AU - Bauer,E, AU - Melchinger,A E, AU - Knapp,S J, AU - Tang,S, AU - Schön,C C, Y1 - 2005/06/10/ PY - 2004/07/30/received PY - 2005/03/12/accepted PY - 2005/6/11/pubmed PY - 2005/12/13/medline PY - 2005/6/11/entrez SP - 233 EP - 42 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 111 IS - 2 N2 - Midstalk rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is an important cause of yield loss in sunflower (Helianthus annuus L.). Objectives of this study were to: (1) estimate the number, genomic positions and genetic effects of quantitative trait loci (QTL) for resistance to midstalk rot in line TUB-5-3234, derived from an interspecific cross; (2) determine congruency of QTL between this line and other sources of resistance; and (3) make inferences about the efficiency of selective genotyping (SG) in detecting QTL conferring midstalk rot resistance in sunflower. Phenotypic data for three resistance (stem lesion, leaf lesion and speed of fungal growth) and two morphological (leaf length and leaf length with petiole) traits were obtained from 434 F3 families from cross CM625 (susceptible) x TUB-5-3234 (resistant) under artificial infection in field experiments across two environments. The SG was applied by choosing the 60 most resistant and the 60 most susceptible F3 families for stem lesion. For genotyping of the respective F2 plants, 78 simple sequence repeat markers were used. Genotypic variances were highly significant for all traits. Heritabilities and genotypic correlations between reMidstalk rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is an important cause of yield loss in sunflower (Helianthus annuus L.). Objectives of this study were to: (1) estimate the number, genomic positions and genetic effects of quantitative trait loci (QTL) for resistance to midstalk rot in line TUB-5-3234, derived from an interspecific cross; (2) determine congruency of QTL between this line and other sources of resistance; and (3) make inferences about the efficiency of selective genotyping (SG) in detecting QTL conferring midstalk rot resistance in sunflower. Phenotypic data for three resistance (stem lesion, leaf lesion and speed of fungal growth) and two morphological (leaf length and leaf length with petiole) traits were obtained from 434 F3 families from cross CM625 (susceptible) x TUB-5-3234 (resistant) under artificial infection in field experiments across two environments. The SG was applied by choosing the 60 most resistant and the 60 most susceptible F3 families for stem lesion. For genotyping of the respective F2 plants, 78 simple sequence repeat markers were used. Genotypic variances were highly significant for all traits. Heritabilities and genotypic correlations between resistance traits were moderate to high. Three to four putative QTL were detected for each resistance trait explaining between 40.8% and 72.7% of the genotypic variance (PTS). Two QTL for stem lesion showed large genetic effects and corroborated earlier findings from the cross NDBLOSsel (resistant) x CM625 (susceptible). Our results suggest that SG can be efficiently used for QTL detection and the analysis of congruency for resistance genes across populations. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/15947909/Identification_and_validation_of_QTL_for_Sclerotinia_midstalk_rot_resistance_in_sunflower_by_selective_genotyping_ L2 - https://dx.doi.org/10.1007/s00122-005-2004-x DB - PRIME DP - Unbound Medicine ER -