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Laser-induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis.
Electrophoresis. 2005 Jun; 26(13):2608-21.E

Abstract

Over the past few years, a large number of studies have been prepared that describe the analysis of peptides and proteins using capillary electrophoresis (CE) and laser-induced fluorescence (LIF). These studies have focused on two general goals: (i) development of automatic, selective and quick separation and detection of mixtures of peptides or proteins; (ii) generation of new methods of quantitation for very low concentrations (nm and subnanomolar) of peptides. These two goals are attained with the use of covalent labelling reactions using a variety of dyes that can be readily excited by the radiation from a commonly available laser or via the use of noncovalent labelling (immunoassay using a labelled antibody or antigen or noncovalent dye interactions). In this review article, we summarize the works which were performed for protein and peptide analysis via CE-LIF.

Authors+Show Affiliations

Lacroix M
Laboratoire des IMRCP, UMR 5623, ERT 1046, Université Paul Sabatier, Toulouse, France.
Poinsot V
No affiliation info available
Fournier C
No affiliation info available
Couderc F
No affiliation info available

MeSH

AdenocarcinomaBenzoatesColonic NeoplasmsElectrophoresis, CapillaryFluorescein-5-isothiocyanateFluorescent DyesFuransHumansLasersNaphthalenesPeptidesProteinsQuinolinesSpectrometry, FluorescenceSubstance P

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

15948219

Citation

Lacroix, Marlene, et al. "Laser-induced Fluorescence Detection Schemes for the Analysis of Proteins and Peptides Using Capillary Electrophoresis." Electrophoresis, vol. 26, no. 13, 2005, pp. 2608-21.
Lacroix M, Poinsot V, Fournier C, et al. Laser-induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis. Electrophoresis. 2005;26(13):2608-21.
Lacroix, M., Poinsot, V., Fournier, C., & Couderc, F. (2005). Laser-induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis. Electrophoresis, 26(13), 2608-21.
Lacroix M, et al. Laser-induced Fluorescence Detection Schemes for the Analysis of Proteins and Peptides Using Capillary Electrophoresis. Electrophoresis. 2005;26(13):2608-21. PubMed PMID: 15948219.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Laser-induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis. AU - Lacroix,Marlene, AU - Poinsot,Véréna, AU - Fournier,Clara, AU - Couderc,François, PY - 2005/6/11/pubmed PY - 2005/9/20/medline PY - 2005/6/11/entrez SP - 2608 EP - 21 JF - Electrophoresis JO - Electrophoresis VL - 26 IS - 13 N2 - Over the past few years, a large number of studies have been prepared that describe the analysis of peptides and proteins using capillary electrophoresis (CE) and laser-induced fluorescence (LIF). These studies have focused on two general goals: (i) development of automatic, selective and quick separation and detection of mixtures of peptides or proteins; (ii) generation of new methods of quantitation for very low concentrations (nm and subnanomolar) of peptides. These two goals are attained with the use of covalent labelling reactions using a variety of dyes that can be readily excited by the radiation from a commonly available laser or via the use of noncovalent labelling (immunoassay using a labelled antibody or antigen or noncovalent dye interactions). In this review article, we summarize the works which were performed for protein and peptide analysis via CE-LIF. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15948219/Laser_induced_fluorescence_detection_schemes_for_the_analysis_of_proteins_and_peptides_using_capillary_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -